Quality Evaluation of Tricholoma matsutake Based on the Nucleic Acid Compounds by UPLC-TOF/MS and UPLC-QqQ/MS
So far, there has been no quality evaluation of Tricholoma matsutake. Nucleic acid compounds are a kind of functional ingredient in T. matsutake that is beneficial to human health. In this study, a UPLC-TOF/MS method was first used to scan and identify the potential nucleic acid compounds in T. mats...
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MDPI AG
2018-12-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/24/1/34 |
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| author | Ying Xue Wei Jin Xian-Shun Xu Li Yong Bin Hu Jing Xiong Xue-Mei Hu Lin-Sen Qing Jing Xie |
| author_facet | Ying Xue Wei Jin Xian-Shun Xu Li Yong Bin Hu Jing Xiong Xue-Mei Hu Lin-Sen Qing Jing Xie |
| author_sort | Ying Xue |
| collection | DOAJ |
| description | So far, there has been no quality evaluation of Tricholoma matsutake. Nucleic acid compounds are a kind of functional ingredient in T. matsutake that is beneficial to human health. In this study, a UPLC-TOF/MS method was first used to scan and identify the potential nucleic acid compounds in T. matsutake. Based on the calculation of the molecular formula and subsequent confirmation by authentic standards, 15 nucleic acid compounds were unambiguously identified: adenosine, cytidine, guanosine, inosine, thymidine, uridine, xanthosine dehydrate, 2′-deoxyadenosine, 2′-deoxycytidine, 2′-deoxyguanosine, 2′-deoxyuridine, adenosine 5′-monophosphate, cytidine 5′-monophosphate, guanosine 5′-monophosphate, and uridine 5′-monophosphate. Then, a UPLC-QqQ/MS method was developed for the subsequent quantitative analysis. After validating the limits of quantification, detection, precision, repeatability, and recovery through a calibration curve, the content of 15 nucleic acid compounds was determined by the proposed UPLC-QqQ/MS method in 80 T. matsutake samples collected from different regions in Sichuan province, Southwest China. After the statistical analysis, we suggest that the total content of nucleic acid compounds in the qualified T. matsutake should be higher than 24.49 mg/100 g. The results indicated that the combined use of UPLC-TOF/MS and UPLC-QqQ/MS is efficient for fast identification and determination of nucleic acid compounds to comprehensively evaluate the quality of T. matsutake. |
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| last_indexed | 2024-12-13T23:58:46Z |
| publishDate | 2018-12-01 |
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| series | Molecules |
| spelling | doaj.art-b8d2c1a6ef614617ab1ac12ba56099c62022-12-21T23:26:27ZengMDPI AGMolecules1420-30492018-12-012413410.3390/molecules24010034molecules24010034Quality Evaluation of Tricholoma matsutake Based on the Nucleic Acid Compounds by UPLC-TOF/MS and UPLC-QqQ/MSYing Xue0Wei Jin1Xian-Shun Xu2Li Yong3Bin Hu4Jing Xiong5Xue-Mei Hu6Lin-Sen Qing7Jing Xie8School of Pharmacy, Collaborative Innovation Center of Sichuan for Elderly Care and Health, Chengdu Medical College, Chengdu 610500, ChinaSichuan Provincial Center for Disease Control and Prevention, Chengdu 610041, ChinaSichuan Provincial Center for Disease Control and Prevention, Chengdu 610041, ChinaSichuan Provincial Center for Disease Control and Prevention, Chengdu 610041, ChinaSichuan Provincial Center for Disease Control and Prevention, Chengdu 610041, ChinaSchool of Pharmacy, Collaborative Innovation Center of Sichuan for Elderly Care and Health, Chengdu Medical College, Chengdu 610500, ChinaSchool of Pharmacy, Collaborative Innovation Center of Sichuan for Elderly Care and Health, Chengdu Medical College, Chengdu 610500, ChinaSchool of Pharmacy, Collaborative Innovation Center of Sichuan for Elderly Care and Health, Chengdu Medical College, Chengdu 610500, ChinaSchool of Pharmacy, Collaborative Innovation Center of Sichuan for Elderly Care and Health, Chengdu Medical College, Chengdu 610500, ChinaSo far, there has been no quality evaluation of Tricholoma matsutake. Nucleic acid compounds are a kind of functional ingredient in T. matsutake that is beneficial to human health. In this study, a UPLC-TOF/MS method was first used to scan and identify the potential nucleic acid compounds in T. matsutake. Based on the calculation of the molecular formula and subsequent confirmation by authentic standards, 15 nucleic acid compounds were unambiguously identified: adenosine, cytidine, guanosine, inosine, thymidine, uridine, xanthosine dehydrate, 2′-deoxyadenosine, 2′-deoxycytidine, 2′-deoxyguanosine, 2′-deoxyuridine, adenosine 5′-monophosphate, cytidine 5′-monophosphate, guanosine 5′-monophosphate, and uridine 5′-monophosphate. Then, a UPLC-QqQ/MS method was developed for the subsequent quantitative analysis. After validating the limits of quantification, detection, precision, repeatability, and recovery through a calibration curve, the content of 15 nucleic acid compounds was determined by the proposed UPLC-QqQ/MS method in 80 T. matsutake samples collected from different regions in Sichuan province, Southwest China. After the statistical analysis, we suggest that the total content of nucleic acid compounds in the qualified T. matsutake should be higher than 24.49 mg/100 g. The results indicated that the combined use of UPLC-TOF/MS and UPLC-QqQ/MS is efficient for fast identification and determination of nucleic acid compounds to comprehensively evaluate the quality of T. matsutake.http://www.mdpi.com/1420-3049/24/1/34Tricholoma matsutakequality evaluationnucleic acid compoundUPLC-TOF/MSUPLC-QqQ/MS |
| spellingShingle | Ying Xue Wei Jin Xian-Shun Xu Li Yong Bin Hu Jing Xiong Xue-Mei Hu Lin-Sen Qing Jing Xie Quality Evaluation of Tricholoma matsutake Based on the Nucleic Acid Compounds by UPLC-TOF/MS and UPLC-QqQ/MS Molecules Tricholoma matsutake quality evaluation nucleic acid compound UPLC-TOF/MS UPLC-QqQ/MS |
| title | Quality Evaluation of Tricholoma matsutake Based on the Nucleic Acid Compounds by UPLC-TOF/MS and UPLC-QqQ/MS |
| title_full | Quality Evaluation of Tricholoma matsutake Based on the Nucleic Acid Compounds by UPLC-TOF/MS and UPLC-QqQ/MS |
| title_fullStr | Quality Evaluation of Tricholoma matsutake Based on the Nucleic Acid Compounds by UPLC-TOF/MS and UPLC-QqQ/MS |
| title_full_unstemmed | Quality Evaluation of Tricholoma matsutake Based on the Nucleic Acid Compounds by UPLC-TOF/MS and UPLC-QqQ/MS |
| title_short | Quality Evaluation of Tricholoma matsutake Based on the Nucleic Acid Compounds by UPLC-TOF/MS and UPLC-QqQ/MS |
| title_sort | quality evaluation of tricholoma matsutake based on the nucleic acid compounds by uplc tof ms and uplc qqq ms |
| topic | Tricholoma matsutake quality evaluation nucleic acid compound UPLC-TOF/MS UPLC-QqQ/MS |
| url | http://www.mdpi.com/1420-3049/24/1/34 |
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