Mapping Stem Rust (Puccinia graminis f. sp. secalis) Resistance in Self-Fertile Winter Rye Populations
Rye stem rust caused by Puccinia graminis f. sp. secalis can be found in all European rye growing regions. When the summers are warm and dry, the disease can cause severe yield losses over large areas. To date only little research was done in Europe to trigger resistance breeding. To our knowledge,...
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Frontiers Media S.A.
2020-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.00667/full |
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| author | Paul Gruner Anne-Kristin Schmitt Kerstin Flath Brigitta Schmiedchen Jakob Eifler Andres Gordillo Malthe Schmidt Viktor Korzun Viktor Korzun Franz-Joachim Fromme Dörthe Siekmann Anna Tratwal Jakub Danielewicz Marek Korbas Karol Marciniak Renata Krysztofik Małgorzata Niewińska Silvia Koch Hans-Peter Piepho Thomas Miedaner |
| author_facet | Paul Gruner Anne-Kristin Schmitt Kerstin Flath Brigitta Schmiedchen Jakob Eifler Andres Gordillo Malthe Schmidt Viktor Korzun Viktor Korzun Franz-Joachim Fromme Dörthe Siekmann Anna Tratwal Jakub Danielewicz Marek Korbas Karol Marciniak Renata Krysztofik Małgorzata Niewińska Silvia Koch Hans-Peter Piepho Thomas Miedaner |
| author_sort | Paul Gruner |
| collection | DOAJ |
| description | Rye stem rust caused by Puccinia graminis f. sp. secalis can be found in all European rye growing regions. When the summers are warm and dry, the disease can cause severe yield losses over large areas. To date only little research was done in Europe to trigger resistance breeding. To our knowledge, all varieties currently registered in Germany are susceptible. In this study, three biparental populations of inbred lines and one testcross population developed for mapping resistance were investigated. Over 2 years, 68–70 genotypes per population were tested, each in three locations. Combining the phenotypic data with genotyping results of a custom 10k Infinium iSelect single nucleotide polymorphism (SNP) array, we identified both quantitatively inherited adult plant resistance and monogenic all-stage resistance. A single resistance gene, tentatively named Pgs1, located at the distal end of chromosome 7R, could be identified in two independently developed populations. With high probability, it is closely linked to a nucleotide-binding leucine-rich repeat (NB-LRR) resistance gene homolog. A marker for a competitive allele-specific polymerase chain reaction (KASP) genotyping assay was designed that could explain 73 and 97% of the genetic variance in each of both populations, respectively. Additional investigation of naturally occurring rye leaf rust (caused by Puccinia recondita ROEBERGE) revealed a gene complex on chromosome 7R. The gene Pgs1 and further identified quantitative trait loci (QTL) have high potential to be used for breeding stem rust resistant rye. |
| first_indexed | 2024-12-13T07:20:29Z |
| format | Article |
| id | doaj.art-b8d3d8f23a894dc2bb26d85cfced9742 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-13T07:20:29Z |
| publishDate | 2020-05-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-b8d3d8f23a894dc2bb26d85cfced97422022-12-21T23:55:27ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-05-011110.3389/fpls.2020.00667536384Mapping Stem Rust (Puccinia graminis f. sp. secalis) Resistance in Self-Fertile Winter Rye PopulationsPaul Gruner0Anne-Kristin Schmitt1Kerstin Flath2Brigitta Schmiedchen3Jakob Eifler4Andres Gordillo5Malthe Schmidt6Viktor Korzun7Viktor Korzun8Franz-Joachim Fromme9Dörthe Siekmann10Anna Tratwal11Jakub Danielewicz12Marek Korbas13Karol Marciniak14Renata Krysztofik15Małgorzata Niewińska16Silvia Koch17Hans-Peter Piepho18Thomas Miedaner19State Plant Breeding Institute, University of Hohenheim, Stuttgart, GermanyInstitute for Plant Protection in Field Crops and Grassland, Julius-Kuehn Institute, Kleinmachnow, GermanyInstitute for Plant Protection in Field Crops and Grassland, Julius-Kuehn Institute, Kleinmachnow, GermanyKWS LOCHOW GmbH, Bergen, GermanyKWS LOCHOW GmbH, Bergen, GermanyKWS LOCHOW GmbH, Bergen, GermanyKWS LOCHOW GmbH, Bergen, GermanyKWS SAAT SE & Co. KGaA, Einbeck, GermanyFederal State Budgetary Institution of Science Federal Research Center “Kazan Scientific Center of Russian Academy of Sciences”, Kazan, RussiaHYBRO Saatzucht GmbH & Co. KG, Schenkenberg, GermanyHYBRO Saatzucht GmbH & Co. KG, Schenkenberg, GermanyInstitute of Plant Protection – National Research Institute, Poznań, PolandInstitute of Plant Protection – National Research Institute, Poznań, PolandInstitute of Plant Protection – National Research Institute, Poznań, PolandDanko Hodowla Roślin Sp. z o.o., Kościan, PolandDanko Hodowla Roślin Sp. z o.o., Kościan, PolandDanko Hodowla Roślin Sp. z o.o., Kościan, PolandState Plant Breeding Institute, University of Hohenheim, Stuttgart, GermanyBiostatistics Unit, Institute of Crop Science, University of Hohenheim, Stuttgart, GermanyState Plant Breeding Institute, University of Hohenheim, Stuttgart, GermanyRye stem rust caused by Puccinia graminis f. sp. secalis can be found in all European rye growing regions. When the summers are warm and dry, the disease can cause severe yield losses over large areas. To date only little research was done in Europe to trigger resistance breeding. To our knowledge, all varieties currently registered in Germany are susceptible. In this study, three biparental populations of inbred lines and one testcross population developed for mapping resistance were investigated. Over 2 years, 68–70 genotypes per population were tested, each in three locations. Combining the phenotypic data with genotyping results of a custom 10k Infinium iSelect single nucleotide polymorphism (SNP) array, we identified both quantitatively inherited adult plant resistance and monogenic all-stage resistance. A single resistance gene, tentatively named Pgs1, located at the distal end of chromosome 7R, could be identified in two independently developed populations. With high probability, it is closely linked to a nucleotide-binding leucine-rich repeat (NB-LRR) resistance gene homolog. A marker for a competitive allele-specific polymerase chain reaction (KASP) genotyping assay was designed that could explain 73 and 97% of the genetic variance in each of both populations, respectively. Additional investigation of naturally occurring rye leaf rust (caused by Puccinia recondita ROEBERGE) revealed a gene complex on chromosome 7R. The gene Pgs1 and further identified quantitative trait loci (QTL) have high potential to be used for breeding stem rust resistant rye.https://www.frontiersin.org/article/10.3389/fpls.2020.00667/fullrustall-stage resistanceadult-plant resistanceQTLmappingmixed model |
| spellingShingle | Paul Gruner Anne-Kristin Schmitt Kerstin Flath Brigitta Schmiedchen Jakob Eifler Andres Gordillo Malthe Schmidt Viktor Korzun Viktor Korzun Franz-Joachim Fromme Dörthe Siekmann Anna Tratwal Jakub Danielewicz Marek Korbas Karol Marciniak Renata Krysztofik Małgorzata Niewińska Silvia Koch Hans-Peter Piepho Thomas Miedaner Mapping Stem Rust (Puccinia graminis f. sp. secalis) Resistance in Self-Fertile Winter Rye Populations Frontiers in Plant Science rust all-stage resistance adult-plant resistance QTL mapping mixed model |
| title | Mapping Stem Rust (Puccinia graminis f. sp. secalis) Resistance in Self-Fertile Winter Rye Populations |
| title_full | Mapping Stem Rust (Puccinia graminis f. sp. secalis) Resistance in Self-Fertile Winter Rye Populations |
| title_fullStr | Mapping Stem Rust (Puccinia graminis f. sp. secalis) Resistance in Self-Fertile Winter Rye Populations |
| title_full_unstemmed | Mapping Stem Rust (Puccinia graminis f. sp. secalis) Resistance in Self-Fertile Winter Rye Populations |
| title_short | Mapping Stem Rust (Puccinia graminis f. sp. secalis) Resistance in Self-Fertile Winter Rye Populations |
| title_sort | mapping stem rust puccinia graminis f sp secalis resistance in self fertile winter rye populations |
| topic | rust all-stage resistance adult-plant resistance QTL mapping mixed model |
| url | https://www.frontiersin.org/article/10.3389/fpls.2020.00667/full |
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