Simultaneous silencing of VEGF and KSP by siRNA cocktail inhibits proliferation and induces apoptosis of hepatocellular carcinoma Hep3B cells
BACKGROUND: Vascular endothelial growth factor (VEGF) is involved in the growth of new blood vessels that feed tumors and kinesin spindle protein (KSP) plays a critical role in mitosis involving in cell proliferation. Simultaneous silencing of VEGF and KSP, an attractive and viable approach in cance...
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| Format: | Article |
| Language: | English |
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BMC
2014-01-01
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100064&lng=en&tlng=en |
| _version_ | 1818505536790855680 |
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| author | Chung Chinh Doan Long Thanh Le Son Nghia Hoang Si Minh Do Dong Van Le |
| author_facet | Chung Chinh Doan Long Thanh Le Son Nghia Hoang Si Minh Do Dong Van Le |
| author_sort | Chung Chinh Doan |
| collection | DOAJ |
| description | BACKGROUND: Vascular endothelial growth factor (VEGF) is involved in the growth of new blood vessels that feed tumors and kinesin spindle protein (KSP) plays a critical role in mitosis involving in cell proliferation. Simultaneous silencing of VEGF and KSP, an attractive and viable approach in cancer, leads on restricting cancer progression. The purpose of this study is to examine the therapeutic potential of dual gene targeted siRNA cocktail on human hepatocellular carcinoma Hep3B cells. RESULTS: The predesigned siRNAs could inhibit VEGF and KSP at mRNA level. siRNA cocktail showed a further downregulation on KSP mRNA and protein levels compared to KSP-siRNA or VEGF-siRNA, but not on VEGF expression. It also exhibited greater suppression on cell proliferation as well as cell migration or invasion capabilities and induction of apoptosis in Hep3B cells than single siRNA simultaneously. This could be explained by the significant downregulation of Cyclin D1, Bcl-2 and Survivin. However, no sigificant difference in the mRNA and protein levels of ANG2, involving inhibition of angiogenesis was found in HUVECs cultured with supernatant of Hep3B cells treated with siRNA cocktail, compared to that of VEGF-siRNA. CONCLUSION: Silencing of VEGF and KSP plays a key role in inhibiting cell proliferation, migration, invasion and inducing apoptosis of Hep3B cells. Simultaneous silencing of VEGF and KSP using siRNA cocktail yields promising results for eradicating hepatocellular carcinoma cells, a new direction for liver cancer treatment. |
| first_indexed | 2024-12-10T21:52:07Z |
| format | Article |
| id | doaj.art-b8dde40ee9de4140ab14285cda68ded8 |
| institution | Directory Open Access Journal |
| issn | 0716-9760 |
| language | English |
| last_indexed | 2024-12-10T21:52:07Z |
| publishDate | 2014-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-b8dde40ee9de4140ab14285cda68ded82022-12-22T01:32:09ZengBMCBiological Research0716-97602014-01-0147011510.1186/0717-6287-47-70S0716-97602014000100064Simultaneous silencing of VEGF and KSP by siRNA cocktail inhibits proliferation and induces apoptosis of hepatocellular carcinoma Hep3B cellsChung Chinh Doan0Long Thanh Le1Son Nghia Hoang2Si Minh Do3Dong Van Le4Vietnam National UniversityVietnam Academy of Science and TechnologyVietnam Academy of Science and TechnologyVietnam National UniversityVietnam Military Medical UniversityBACKGROUND: Vascular endothelial growth factor (VEGF) is involved in the growth of new blood vessels that feed tumors and kinesin spindle protein (KSP) plays a critical role in mitosis involving in cell proliferation. Simultaneous silencing of VEGF and KSP, an attractive and viable approach in cancer, leads on restricting cancer progression. The purpose of this study is to examine the therapeutic potential of dual gene targeted siRNA cocktail on human hepatocellular carcinoma Hep3B cells. RESULTS: The predesigned siRNAs could inhibit VEGF and KSP at mRNA level. siRNA cocktail showed a further downregulation on KSP mRNA and protein levels compared to KSP-siRNA or VEGF-siRNA, but not on VEGF expression. It also exhibited greater suppression on cell proliferation as well as cell migration or invasion capabilities and induction of apoptosis in Hep3B cells than single siRNA simultaneously. This could be explained by the significant downregulation of Cyclin D1, Bcl-2 and Survivin. However, no sigificant difference in the mRNA and protein levels of ANG2, involving inhibition of angiogenesis was found in HUVECs cultured with supernatant of Hep3B cells treated with siRNA cocktail, compared to that of VEGF-siRNA. CONCLUSION: Silencing of VEGF and KSP plays a key role in inhibiting cell proliferation, migration, invasion and inducing apoptosis of Hep3B cells. Simultaneous silencing of VEGF and KSP using siRNA cocktail yields promising results for eradicating hepatocellular carcinoma cells, a new direction for liver cancer treatment.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100064&lng=en&tlng=enVascular endothelial cell growth factor (VEGF)Kinesin spindle protein (KSP)siRNA cocktailProliferationApoptosisHepatocellular carcinoma |
| spellingShingle | Chung Chinh Doan Long Thanh Le Son Nghia Hoang Si Minh Do Dong Van Le Simultaneous silencing of VEGF and KSP by siRNA cocktail inhibits proliferation and induces apoptosis of hepatocellular carcinoma Hep3B cells Biological Research Vascular endothelial cell growth factor (VEGF) Kinesin spindle protein (KSP) siRNA cocktail Proliferation Apoptosis Hepatocellular carcinoma |
| title | Simultaneous silencing of VEGF and KSP by siRNA cocktail inhibits proliferation and induces apoptosis of hepatocellular carcinoma Hep3B cells |
| title_full | Simultaneous silencing of VEGF and KSP by siRNA cocktail inhibits proliferation and induces apoptosis of hepatocellular carcinoma Hep3B cells |
| title_fullStr | Simultaneous silencing of VEGF and KSP by siRNA cocktail inhibits proliferation and induces apoptosis of hepatocellular carcinoma Hep3B cells |
| title_full_unstemmed | Simultaneous silencing of VEGF and KSP by siRNA cocktail inhibits proliferation and induces apoptosis of hepatocellular carcinoma Hep3B cells |
| title_short | Simultaneous silencing of VEGF and KSP by siRNA cocktail inhibits proliferation and induces apoptosis of hepatocellular carcinoma Hep3B cells |
| title_sort | simultaneous silencing of vegf and ksp by sirna cocktail inhibits proliferation and induces apoptosis of hepatocellular carcinoma hep3b cells |
| topic | Vascular endothelial cell growth factor (VEGF) Kinesin spindle protein (KSP) siRNA cocktail Proliferation Apoptosis Hepatocellular carcinoma |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100064&lng=en&tlng=en |
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