Genomic, Antimicrobial, and Aphicidal Traits of <i>Bacillus velezensis</i> ATR2, and Its Biocontrol Potential against Ginger Rhizome Rot Disease Caused by <i>Bacillus pumilus</i>
Ginger rhizome rot disease, caused by the pathogen <i>Bacillus</i><i>pumilus</i> GR8, could result in severe rot of ginger rhizomes and heavily threaten ginger production. In this study, we identified and characterized a new <i>Bacillus velezensis</i> strain, desi...
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MDPI AG
2021-12-01
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| author | Leiqin Liang Yajuan Fu Sangsang Deng Yan Wu Meiying Gao |
| author_facet | Leiqin Liang Yajuan Fu Sangsang Deng Yan Wu Meiying Gao |
| author_sort | Leiqin Liang |
| collection | DOAJ |
| description | Ginger rhizome rot disease, caused by the pathogen <i>Bacillus</i><i>pumilus</i> GR8, could result in severe rot of ginger rhizomes and heavily threaten ginger production. In this study, we identified and characterized a new <i>Bacillus velezensis</i> strain, designated ATR2. Genome analysis revealed <i>B. velezensis</i> ATR2 harbored a series of genes closely related to promoting plant growth and triggering plant immunity. Meanwhile, ten gene clusters involved in the biosynthesis of various secondary metabolites (surfactin, bacillomycin, fengycin, bacillibactin, bacilysin, difficidin, macrolactin, bacillaene, plantazolicin, and amylocyclicin) and two clusters encoding a putative lipopeptide and a putative phosphonate which might be explored as novel bioactive compounds were also present in the ATR2 genome. Moreover, <i>B. velezensis</i> ATR2 showed excellent antagonistic activities against multiple plant pathogenic bacteria, plant pathogenic fungi, human pathogenic bacteria, and human pathogenic fungus. <i>B. velezensis</i> ATR2 was also efficacious in control of aphids. The antagonistic compound from <i>B. velezensis</i> ATR2 against <i>B.</i><i>pumilus</i> GR8 was purified and identified as bacillomycin D. In addition, <i>B. velezensis</i> ATR2 exhibited excellent biocontrol efficacy against ginger rhizome rot disease on ginger slices. These findings showed the potential of further applications of <i>B. velezensis</i> ATR2 as a biocontrol agent in agricultural diseases and pests management. |
| first_indexed | 2024-03-10T00:54:08Z |
| format | Article |
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| language | English |
| last_indexed | 2024-03-10T00:54:08Z |
| publishDate | 2021-12-01 |
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| series | Microorganisms |
| spelling | doaj.art-b8de84090fa64594ac6122507fa484562023-11-23T14:46:45ZengMDPI AGMicroorganisms2076-26072021-12-011016310.3390/microorganisms10010063Genomic, Antimicrobial, and Aphicidal Traits of <i>Bacillus velezensis</i> ATR2, and Its Biocontrol Potential against Ginger Rhizome Rot Disease Caused by <i>Bacillus pumilus</i>Leiqin Liang0Yajuan Fu1Sangsang Deng2Yan Wu3Meiying Gao4Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, ChinaWuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, ChinaWuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, ChinaWuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, ChinaWuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, ChinaGinger rhizome rot disease, caused by the pathogen <i>Bacillus</i><i>pumilus</i> GR8, could result in severe rot of ginger rhizomes and heavily threaten ginger production. In this study, we identified and characterized a new <i>Bacillus velezensis</i> strain, designated ATR2. Genome analysis revealed <i>B. velezensis</i> ATR2 harbored a series of genes closely related to promoting plant growth and triggering plant immunity. Meanwhile, ten gene clusters involved in the biosynthesis of various secondary metabolites (surfactin, bacillomycin, fengycin, bacillibactin, bacilysin, difficidin, macrolactin, bacillaene, plantazolicin, and amylocyclicin) and two clusters encoding a putative lipopeptide and a putative phosphonate which might be explored as novel bioactive compounds were also present in the ATR2 genome. Moreover, <i>B. velezensis</i> ATR2 showed excellent antagonistic activities against multiple plant pathogenic bacteria, plant pathogenic fungi, human pathogenic bacteria, and human pathogenic fungus. <i>B. velezensis</i> ATR2 was also efficacious in control of aphids. The antagonistic compound from <i>B. velezensis</i> ATR2 against <i>B.</i><i>pumilus</i> GR8 was purified and identified as bacillomycin D. In addition, <i>B. velezensis</i> ATR2 exhibited excellent biocontrol efficacy against ginger rhizome rot disease on ginger slices. These findings showed the potential of further applications of <i>B. velezensis</i> ATR2 as a biocontrol agent in agricultural diseases and pests management.https://www.mdpi.com/2076-2607/10/1/63<i>Bacillus velezensis</i>ginger rhizome rot diseasegenome analysisantimicrobial activityaphicidal activitybacillomycin D |
| spellingShingle | Leiqin Liang Yajuan Fu Sangsang Deng Yan Wu Meiying Gao Genomic, Antimicrobial, and Aphicidal Traits of <i>Bacillus velezensis</i> ATR2, and Its Biocontrol Potential against Ginger Rhizome Rot Disease Caused by <i>Bacillus pumilus</i> Microorganisms <i>Bacillus velezensis</i> ginger rhizome rot disease genome analysis antimicrobial activity aphicidal activity bacillomycin D |
| title | Genomic, Antimicrobial, and Aphicidal Traits of <i>Bacillus velezensis</i> ATR2, and Its Biocontrol Potential against Ginger Rhizome Rot Disease Caused by <i>Bacillus pumilus</i> |
| title_full | Genomic, Antimicrobial, and Aphicidal Traits of <i>Bacillus velezensis</i> ATR2, and Its Biocontrol Potential against Ginger Rhizome Rot Disease Caused by <i>Bacillus pumilus</i> |
| title_fullStr | Genomic, Antimicrobial, and Aphicidal Traits of <i>Bacillus velezensis</i> ATR2, and Its Biocontrol Potential against Ginger Rhizome Rot Disease Caused by <i>Bacillus pumilus</i> |
| title_full_unstemmed | Genomic, Antimicrobial, and Aphicidal Traits of <i>Bacillus velezensis</i> ATR2, and Its Biocontrol Potential against Ginger Rhizome Rot Disease Caused by <i>Bacillus pumilus</i> |
| title_short | Genomic, Antimicrobial, and Aphicidal Traits of <i>Bacillus velezensis</i> ATR2, and Its Biocontrol Potential against Ginger Rhizome Rot Disease Caused by <i>Bacillus pumilus</i> |
| title_sort | genomic antimicrobial and aphicidal traits of i bacillus velezensis i atr2 and its biocontrol potential against ginger rhizome rot disease caused by i bacillus pumilus i |
| topic | <i>Bacillus velezensis</i> ginger rhizome rot disease genome analysis antimicrobial activity aphicidal activity bacillomycin D |
| url | https://www.mdpi.com/2076-2607/10/1/63 |
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