Alterations to oxidative stress markers in dogs after a short-term stress during transport*
While methods to evaluate antioxidant capacity in animals exist, one problem with the models is induction of oxidative stress. It is necessary to promote a great enough challenge to induce measurable alterations to oxidative parameters while ensuring the protocol is compatible with animal welfare. T...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2014-01-01
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| Series: | Journal of Nutritional Science |
| Subjects: | |
| Online Access: | https://www.cambridge.org/core/product/identifier/S2048679014000470/type/journal_article |
| _version_ | 1811156014318223360 |
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| author | Chayanne S. Ferreira Ricardo S. Vasconcellos Raquel S. Pedreira Flavio L. Silva Fabiano C. Sá Fernanda S. A. Kroll Ana P. J. Maria Katiani S. Venturini Aulus C. Carciofi |
| author_facet | Chayanne S. Ferreira Ricardo S. Vasconcellos Raquel S. Pedreira Flavio L. Silva Fabiano C. Sá Fernanda S. A. Kroll Ana P. J. Maria Katiani S. Venturini Aulus C. Carciofi |
| author_sort | Chayanne S. Ferreira |
| collection | DOAJ |
| description | While methods to evaluate antioxidant capacity in animals exist, one problem with the models is induction of oxidative stress. It is necessary to promote a great enough challenge to induce measurable alterations to oxidative parameters while ensuring the protocol is compatible with animal welfare. The aim of the present study was to evaluate caged transport as a viable short-term stress that would significantly affect oxidative parameters. Twenty adult Beagle dogs, maintained on the same diet for 60 d prior to the transport, were included in the study. To simulate the stress, the dogs were housed in pairs in transport cages (1·0 m × 1·0 m × 1·5 m), placed on a truck coupled to a trailer and transported for a period of 15 min. Blood collection was performed immediately before and again 3 h after the transportation to evaluate oxidative parameters in blood serum, including thiobarbituric acid reactive substances (TBARS), total antioxidant capacity (TAC), sequestration activity of the radical 2,2-diphenyl-1-picryl-hydrazyl (DPPH•), protein carbonylation (PC), total sulfhydryl groups (SH), alpha-tocopherol (αToc) and retinol (Ret). PC, SH and αToc were not significantly changed in the study; however, TBARS, TAC and DPPH increased, whereas Ret decreased after the transport. Although the lack of a control group of dogs not submitted to transport is a limitation to be considered, we conclude that the transport model is effective in inducing an antioxidant response in dogs and relevant blood parameters show sensitivity to this proposed model. |
| first_indexed | 2024-04-10T04:44:17Z |
| format | Article |
| id | doaj.art-b8df47387db74cf9989bb7b54d94cb1f |
| institution | Directory Open Access Journal |
| issn | 2048-6790 |
| language | English |
| last_indexed | 2024-04-10T04:44:17Z |
| publishDate | 2014-01-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Journal of Nutritional Science |
| spelling | doaj.art-b8df47387db74cf9989bb7b54d94cb1f2023-03-09T12:38:43ZengCambridge University PressJournal of Nutritional Science2048-67902014-01-01310.1017/jns.2014.47Alterations to oxidative stress markers in dogs after a short-term stress during transport*Chayanne S. Ferreira0Ricardo S. Vasconcellos1Raquel S. Pedreira2Flavio L. Silva3Fabiano C. Sá4Fernanda S. A. Kroll5Ana P. J. Maria6Katiani S. Venturini7Aulus C. Carciofi8College of Agrarian and Veterinarian Sciences (FCAV), São Paulo State University (UNESP), Via de Acesso Professor Paulo Donato Castellane, s/n Jaboticabal, 14.884-900 SP, BrazilDepartment of Animal Science (DZO), State University of Maringá (UEM), Av. Colombo, 5790, Maringá 87020-900, PR, BrazilCollege of Agrarian and Veterinarian Sciences (FCAV), São Paulo State University (UNESP), Via de Acesso Professor Paulo Donato Castellane, s/n Jaboticabal, 14.884-900 SP, BrazilCollege of Agrarian and Veterinarian Sciences (FCAV), São Paulo State University (UNESP), Via de Acesso Professor Paulo Donato Castellane, s/n Jaboticabal, 14.884-900 SP, BrazilCollege of Agrarian and Veterinarian Sciences (FCAV), São Paulo State University (UNESP), Via de Acesso Professor Paulo Donato Castellane, s/n Jaboticabal, 14.884-900 SP, BrazilCollege of Agrarian and Veterinarian Sciences (FCAV), São Paulo State University (UNESP), Via de Acesso Professor Paulo Donato Castellane, s/n Jaboticabal, 14.884-900 SP, BrazilCollege of Agrarian and Veterinarian Sciences (FCAV), São Paulo State University (UNESP), Via de Acesso Professor Paulo Donato Castellane, s/n Jaboticabal, 14.884-900 SP, BrazilCollege of Agrarian and Veterinarian Sciences (FCAV), São Paulo State University (UNESP), Via de Acesso Professor Paulo Donato Castellane, s/n Jaboticabal, 14.884-900 SP, BrazilCollege of Agrarian and Veterinarian Sciences (FCAV), São Paulo State University (UNESP), Via de Acesso Professor Paulo Donato Castellane, s/n Jaboticabal, 14.884-900 SP, BrazilWhile methods to evaluate antioxidant capacity in animals exist, one problem with the models is induction of oxidative stress. It is necessary to promote a great enough challenge to induce measurable alterations to oxidative parameters while ensuring the protocol is compatible with animal welfare. The aim of the present study was to evaluate caged transport as a viable short-term stress that would significantly affect oxidative parameters. Twenty adult Beagle dogs, maintained on the same diet for 60 d prior to the transport, were included in the study. To simulate the stress, the dogs were housed in pairs in transport cages (1·0 m × 1·0 m × 1·5 m), placed on a truck coupled to a trailer and transported for a period of 15 min. Blood collection was performed immediately before and again 3 h after the transportation to evaluate oxidative parameters in blood serum, including thiobarbituric acid reactive substances (TBARS), total antioxidant capacity (TAC), sequestration activity of the radical 2,2-diphenyl-1-picryl-hydrazyl (DPPH•), protein carbonylation (PC), total sulfhydryl groups (SH), alpha-tocopherol (αToc) and retinol (Ret). PC, SH and αToc were not significantly changed in the study; however, TBARS, TAC and DPPH increased, whereas Ret decreased after the transport. Although the lack of a control group of dogs not submitted to transport is a limitation to be considered, we conclude that the transport model is effective in inducing an antioxidant response in dogs and relevant blood parameters show sensitivity to this proposed model.https://www.cambridge.org/core/product/identifier/S2048679014000470/type/journal_articleAlpha-tocopherolAntioxidantsCanine nutritionLipoperoxidationThiobarbituric acid |
| spellingShingle | Chayanne S. Ferreira Ricardo S. Vasconcellos Raquel S. Pedreira Flavio L. Silva Fabiano C. Sá Fernanda S. A. Kroll Ana P. J. Maria Katiani S. Venturini Aulus C. Carciofi Alterations to oxidative stress markers in dogs after a short-term stress during transport* Journal of Nutritional Science Alpha-tocopherol Antioxidants Canine nutrition Lipoperoxidation Thiobarbituric acid |
| title | Alterations to oxidative stress markers in dogs after a short-term stress during transport* |
| title_full | Alterations to oxidative stress markers in dogs after a short-term stress during transport* |
| title_fullStr | Alterations to oxidative stress markers in dogs after a short-term stress during transport* |
| title_full_unstemmed | Alterations to oxidative stress markers in dogs after a short-term stress during transport* |
| title_short | Alterations to oxidative stress markers in dogs after a short-term stress during transport* |
| title_sort | alterations to oxidative stress markers in dogs after a short term stress during transport |
| topic | Alpha-tocopherol Antioxidants Canine nutrition Lipoperoxidation Thiobarbituric acid |
| url | https://www.cambridge.org/core/product/identifier/S2048679014000470/type/journal_article |
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