MiR-550a-3p restores damaged vascular smooth muscle cells by inhibiting thrombomodulin in an <em>in vitro</em> atherosclerosis model
Thrombomodulin (TM) is involved in the pathological process of atherosclerosis; however, the underlying mechanism remains unclear. Oxidised low-density lipoprotein (Ox-LDL; 100 μg/mL) was used to induce human vascular smooth muscle cells (HVSMCs) into a stable atherosclerotic cell model. The expres...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2022-07-01
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| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | https://ejh.it/index.php/ejh/article/view/3429 |
| _version_ | 1811296222679400448 |
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| author | Shiyuan Chen Longfei Zhang Benchi Feng Wei Wang Delang Liu Xinyu Zhao Chaowen Yu Xiaogao Wang Yong Gao |
| author_facet | Shiyuan Chen Longfei Zhang Benchi Feng Wei Wang Delang Liu Xinyu Zhao Chaowen Yu Xiaogao Wang Yong Gao |
| author_sort | Shiyuan Chen |
| collection | DOAJ |
| description |
Thrombomodulin (TM) is involved in the pathological process of atherosclerosis; however, the underlying mechanism remains unclear. Oxidised low-density lipoprotein (Ox-LDL; 100 μg/mL) was used to induce human vascular smooth muscle cells (HVSMCs) into a stable atherosclerotic cell model. The expression levels of miR-550a-3p and TM were detected by real-time reverse transcription-polymerase chain reaction. Cell proliferation was estimated using CCK8 and EDU assays. Wound scratch and transwell assays were used to measure the ability of cells to invade and migrate. Propidium iodide fluorescence-activated cell sorting was used to detect apoptosis and cell cycle changes. A dual-luciferase reporter assay was performed to determine the binding of miR-550a-3p to TM. Our results suggested the successful development of a cellular atherosclerosis model. Our data revealed that TM overexpression significantly promoted the proliferation, invasion, migration, and apoptosis of HVSMCs as well as cell cycle changes. Upregulation of miR-550a-3p inhibited the growth and metastasis of HVSMCs. Furthermore, miR-550a-3p was confirmed to be a direct target of TM. Restoration of miR-550a-3p expression rescued the effects of TM overexpression. Thus, miR-550a-3p might play a role in atherosclerosis and, for the first time, normalised the function of injured vascular endothelial cells by simultaneous transfection of TM and miR-550a-3p. These results suggest that the miR-550a-3p/TM axis is a potential therapeutic target for atherosclerosis.
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| first_indexed | 2024-04-13T05:45:03Z |
| format | Article |
| id | doaj.art-b8e0802cd15c45aea8f6d39c873daab8 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-04-13T05:45:03Z |
| publishDate | 2022-07-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-b8e0802cd15c45aea8f6d39c873daab82022-12-22T02:59:58ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062022-07-0166310.4081/ejh.2022.3429MiR-550a-3p restores damaged vascular smooth muscle cells by inhibiting thrombomodulin in an <em>in vitro</em> atherosclerosis model Shiyuan Chen0Longfei Zhang1Benchi Feng2Wei Wang3Delang Liu4Xinyu Zhao5Chaowen Yu6Xiaogao Wang7Yong Gao8The First Clinical College, Jinan University, Guangzhou, Guangdong; Department of Vascular Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, AnhuiDepartment of Vascular Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, AnhuiDepartment of Vascular Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, AnhuiDepartment of Oncological Surgery, The Second Affiliated Hospital of Bengbu Medical College, Bengbu, AnhuiDepartment of Vascular Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, AnhuiDepartment of Vascular Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, AnhuiDepartment of Vascular Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, AnhuiDepartment of Vascular Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, AnhuiThe First Clinical College, Jinan University, Guangzhou, Guangdong; Department of Vascular Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui Thrombomodulin (TM) is involved in the pathological process of atherosclerosis; however, the underlying mechanism remains unclear. Oxidised low-density lipoprotein (Ox-LDL; 100 μg/mL) was used to induce human vascular smooth muscle cells (HVSMCs) into a stable atherosclerotic cell model. The expression levels of miR-550a-3p and TM were detected by real-time reverse transcription-polymerase chain reaction. Cell proliferation was estimated using CCK8 and EDU assays. Wound scratch and transwell assays were used to measure the ability of cells to invade and migrate. Propidium iodide fluorescence-activated cell sorting was used to detect apoptosis and cell cycle changes. A dual-luciferase reporter assay was performed to determine the binding of miR-550a-3p to TM. Our results suggested the successful development of a cellular atherosclerosis model. Our data revealed that TM overexpression significantly promoted the proliferation, invasion, migration, and apoptosis of HVSMCs as well as cell cycle changes. Upregulation of miR-550a-3p inhibited the growth and metastasis of HVSMCs. Furthermore, miR-550a-3p was confirmed to be a direct target of TM. Restoration of miR-550a-3p expression rescued the effects of TM overexpression. Thus, miR-550a-3p might play a role in atherosclerosis and, for the first time, normalised the function of injured vascular endothelial cells by simultaneous transfection of TM and miR-550a-3p. These results suggest that the miR-550a-3p/TM axis is a potential therapeutic target for atherosclerosis. https://ejh.it/index.php/ejh/article/view/3429ThrombomodulinatherosclerosismiR-550a-3p |
| spellingShingle | Shiyuan Chen Longfei Zhang Benchi Feng Wei Wang Delang Liu Xinyu Zhao Chaowen Yu Xiaogao Wang Yong Gao MiR-550a-3p restores damaged vascular smooth muscle cells by inhibiting thrombomodulin in an <em>in vitro</em> atherosclerosis model European Journal of Histochemistry Thrombomodulin atherosclerosis miR-550a-3p |
| title | MiR-550a-3p restores damaged vascular smooth muscle cells by inhibiting thrombomodulin in an <em>in vitro</em> atherosclerosis model |
| title_full | MiR-550a-3p restores damaged vascular smooth muscle cells by inhibiting thrombomodulin in an <em>in vitro</em> atherosclerosis model |
| title_fullStr | MiR-550a-3p restores damaged vascular smooth muscle cells by inhibiting thrombomodulin in an <em>in vitro</em> atherosclerosis model |
| title_full_unstemmed | MiR-550a-3p restores damaged vascular smooth muscle cells by inhibiting thrombomodulin in an <em>in vitro</em> atherosclerosis model |
| title_short | MiR-550a-3p restores damaged vascular smooth muscle cells by inhibiting thrombomodulin in an <em>in vitro</em> atherosclerosis model |
| title_sort | mir 550a 3p restores damaged vascular smooth muscle cells by inhibiting thrombomodulin in an em in vitro em atherosclerosis model |
| topic | Thrombomodulin atherosclerosis miR-550a-3p |
| url | https://ejh.it/index.php/ejh/article/view/3429 |
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