Novel insights on the potential activity of propolis and wheat germ oil against chronic toxoplasmosis in experimentally infected mice
The use of apitherapy and natural herbal medicines for combating toxoplasmosis has garnered major attention from many researchers. However, there is no available information regarding the potential use of a combination of propolis and wheat germ oil (WGO) in the treatment of toxoplasmosis. In the pr...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Biomedicine & Pharmacotherapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332222012008 |
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| author | Ehab Kotb Elmahallawy Hassan Ali Mohamed El Fadaly Ashraf H. Soror Fatma Abo Zakaib Ali Khaled A. Abd El-Razik Yousef A. Soliman Abdulsalam A.M. Alkhaldi Nisreen Khalid Aref Albezrah Ashraf Mohamed Barakat |
| author_facet | Ehab Kotb Elmahallawy Hassan Ali Mohamed El Fadaly Ashraf H. Soror Fatma Abo Zakaib Ali Khaled A. Abd El-Razik Yousef A. Soliman Abdulsalam A.M. Alkhaldi Nisreen Khalid Aref Albezrah Ashraf Mohamed Barakat |
| author_sort | Ehab Kotb Elmahallawy |
| collection | DOAJ |
| description | The use of apitherapy and natural herbal medicines for combating toxoplasmosis has garnered major attention from many researchers. However, there is no available information regarding the potential use of a combination of propolis and wheat germ oil (WGO) in the treatment of toxoplasmosis. In the present study, the potential effects of propolis, WGO, and their combination in the treatment of chronic toxoplasmosis in Swiss albino mice were investigated. Following induction of chronic toxoplasmosis, the potential antiparasitic effects of these substances were evaluated by parasitological assessment and by counting of Toxoplasma cysts. Additionally, the effects of the treatments on parasite loads were analyzed using TaqMan real-time quantitative PCR targeting the Toxoplasma P29 gene followed by investigation of the major histopathological changes in the brain, uterus, and kidney. Interestingly, the combination of propolis and WGO significantly (P ≤ 0.05) decreased the parasite burden in experimental animals compared with burdens seen in groups treated with propolis or WGO alone. Furthermore, the quantification of the DNA concentrations of Toxoplasma P29 gene after the treatment with propolis and WGO revealed a reduction in parasite load in treated groups versus the control group (infected untreated animals). Importantly, the severity of histopathological lesions was significantly (P ≤ 0.05) improved following treatment with propolis and WGO. Collectively, the present study indicated a potential novel role for propolis and WGO as an active apitherapy and natural herbal medication for treating chronic toxoplasmosis, combat the disease, and which could also help overcome the side effects of chemical drugs. |
| first_indexed | 2024-04-13T09:27:04Z |
| format | Article |
| id | doaj.art-b8e192ca99c040d98a09754d6664caf0 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-04-13T09:27:04Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-b8e192ca99c040d98a09754d6664caf02022-12-22T02:52:24ZengElsevierBiomedicine & Pharmacotherapy0753-33222022-12-01156113811Novel insights on the potential activity of propolis and wheat germ oil against chronic toxoplasmosis in experimentally infected miceEhab Kotb Elmahallawy0Hassan Ali Mohamed El Fadaly1Ashraf H. Soror2Fatma Abo Zakaib Ali3Khaled A. Abd El-Razik4Yousef A. Soliman5Abdulsalam A.M. Alkhaldi6Nisreen Khalid Aref Albezrah7Ashraf Mohamed Barakat8Department of Zoonoses, Faculty of Veterinary Medicine, Sohag University, Sohag 82524, Egypt; Corresponding author.Department of Zoonotic Diseases, National Research Centre, El Buhouth st., Dokki, Giza 12622, EgyptDepartment of Animal Reproduction, Veterinary Research Institute, National Research Centre, Giza, EgyptDepartment of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, Sohag University, EgyptDepartment of Animal Reproduction, Veterinary Research Institute, National Research Centre, Giza, EgyptCentral Lab for Evaluation of Vet. Biologics (CLEVB), Abbasia, Cairo, EgyptBiology Department, College of Science, Jouf University, Sakaka 72388, Saudi ArabiaDepartment of Obstetrics and Gynecology, College of Medicine, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Zoonotic Diseases, National Research Centre, El Buhouth st., Dokki, Giza 12622, EgyptThe use of apitherapy and natural herbal medicines for combating toxoplasmosis has garnered major attention from many researchers. However, there is no available information regarding the potential use of a combination of propolis and wheat germ oil (WGO) in the treatment of toxoplasmosis. In the present study, the potential effects of propolis, WGO, and their combination in the treatment of chronic toxoplasmosis in Swiss albino mice were investigated. Following induction of chronic toxoplasmosis, the potential antiparasitic effects of these substances were evaluated by parasitological assessment and by counting of Toxoplasma cysts. Additionally, the effects of the treatments on parasite loads were analyzed using TaqMan real-time quantitative PCR targeting the Toxoplasma P29 gene followed by investigation of the major histopathological changes in the brain, uterus, and kidney. Interestingly, the combination of propolis and WGO significantly (P ≤ 0.05) decreased the parasite burden in experimental animals compared with burdens seen in groups treated with propolis or WGO alone. Furthermore, the quantification of the DNA concentrations of Toxoplasma P29 gene after the treatment with propolis and WGO revealed a reduction in parasite load in treated groups versus the control group (infected untreated animals). Importantly, the severity of histopathological lesions was significantly (P ≤ 0.05) improved following treatment with propolis and WGO. Collectively, the present study indicated a potential novel role for propolis and WGO as an active apitherapy and natural herbal medication for treating chronic toxoplasmosis, combat the disease, and which could also help overcome the side effects of chemical drugs.http://www.sciencedirect.com/science/article/pii/S0753332222012008Chronic toxoplasmosisPropolisWheat germ oilHistopathologyReal time polymerase chain reaction |
| spellingShingle | Ehab Kotb Elmahallawy Hassan Ali Mohamed El Fadaly Ashraf H. Soror Fatma Abo Zakaib Ali Khaled A. Abd El-Razik Yousef A. Soliman Abdulsalam A.M. Alkhaldi Nisreen Khalid Aref Albezrah Ashraf Mohamed Barakat Novel insights on the potential activity of propolis and wheat germ oil against chronic toxoplasmosis in experimentally infected mice Biomedicine & Pharmacotherapy Chronic toxoplasmosis Propolis Wheat germ oil Histopathology Real time polymerase chain reaction |
| title | Novel insights on the potential activity of propolis and wheat germ oil against chronic toxoplasmosis in experimentally infected mice |
| title_full | Novel insights on the potential activity of propolis and wheat germ oil against chronic toxoplasmosis in experimentally infected mice |
| title_fullStr | Novel insights on the potential activity of propolis and wheat germ oil against chronic toxoplasmosis in experimentally infected mice |
| title_full_unstemmed | Novel insights on the potential activity of propolis and wheat germ oil against chronic toxoplasmosis in experimentally infected mice |
| title_short | Novel insights on the potential activity of propolis and wheat germ oil against chronic toxoplasmosis in experimentally infected mice |
| title_sort | novel insights on the potential activity of propolis and wheat germ oil against chronic toxoplasmosis in experimentally infected mice |
| topic | Chronic toxoplasmosis Propolis Wheat germ oil Histopathology Real time polymerase chain reaction |
| url | http://www.sciencedirect.com/science/article/pii/S0753332222012008 |
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