Genetic and epigenetic control of gene expression by CRISPR–Cas systems [version 1; referees: 3 approved]
The discovery and adaption of bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems has revolutionized the way researchers edit genomes. Engineering of catalytically inactivated Cas variants (nuclease-deficient or nuclease-deactivated [dCas]) co...
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| Format: | Article |
| Language: | English |
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F1000 Research Ltd
2017-05-01
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| Series: | F1000Research |
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| Online Access: | https://f1000research.com/articles/6-747/v1 |
| _version_ | 1818056665064275968 |
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| author | Albert Lo Lei Qi |
| author_facet | Albert Lo Lei Qi |
| author_sort | Albert Lo |
| collection | DOAJ |
| description | The discovery and adaption of bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems has revolutionized the way researchers edit genomes. Engineering of catalytically inactivated Cas variants (nuclease-deficient or nuclease-deactivated [dCas]) combined with transcriptional repressors, activators, or epigenetic modifiers enable sequence-specific regulation of gene expression and chromatin state. These CRISPR–Cas-based technologies have contributed to the rapid development of disease models and functional genomics screening approaches, which can facilitate genetic target identification and drug discovery. In this short review, we will cover recent advances of CRISPR–dCas9 systems and their use for transcriptional repression and activation, epigenome editing, and engineered synthetic circuits for complex control of the mammalian genome. |
| first_indexed | 2024-12-10T12:32:27Z |
| format | Article |
| id | doaj.art-b8e31f97f99f4d23a594771e3683d0a3 |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-12-10T12:32:27Z |
| publishDate | 2017-05-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj.art-b8e31f97f99f4d23a594771e3683d0a32022-12-22T01:48:47ZengF1000 Research LtdF1000Research2046-14022017-05-01610.12688/f1000research.11113.111989Genetic and epigenetic control of gene expression by CRISPR–Cas systems [version 1; referees: 3 approved]Albert Lo0Lei Qi1Department of Bioengineering, Stanford University, Stanford, CA 94305, USAChEM-H, Stanford University, Stanford, CA 94305, USAThe discovery and adaption of bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems has revolutionized the way researchers edit genomes. Engineering of catalytically inactivated Cas variants (nuclease-deficient or nuclease-deactivated [dCas]) combined with transcriptional repressors, activators, or epigenetic modifiers enable sequence-specific regulation of gene expression and chromatin state. These CRISPR–Cas-based technologies have contributed to the rapid development of disease models and functional genomics screening approaches, which can facilitate genetic target identification and drug discovery. In this short review, we will cover recent advances of CRISPR–dCas9 systems and their use for transcriptional repression and activation, epigenome editing, and engineered synthetic circuits for complex control of the mammalian genome.https://f1000research.com/articles/6-747/v1BiocatalysisChemical Biology of the CellControl of Gene ExpressionGenomicsMicrobial Evolution & GenomicsMicrobial Physiology & MetabolismPlant Genomes & EvolutionProtein Chemistry & Proteomics |
| spellingShingle | Albert Lo Lei Qi Genetic and epigenetic control of gene expression by CRISPR–Cas systems [version 1; referees: 3 approved] F1000Research Biocatalysis Chemical Biology of the Cell Control of Gene Expression Genomics Microbial Evolution & Genomics Microbial Physiology & Metabolism Plant Genomes & Evolution Protein Chemistry & Proteomics |
| title | Genetic and epigenetic control of gene expression by CRISPR–Cas systems [version 1; referees: 3 approved] |
| title_full | Genetic and epigenetic control of gene expression by CRISPR–Cas systems [version 1; referees: 3 approved] |
| title_fullStr | Genetic and epigenetic control of gene expression by CRISPR–Cas systems [version 1; referees: 3 approved] |
| title_full_unstemmed | Genetic and epigenetic control of gene expression by CRISPR–Cas systems [version 1; referees: 3 approved] |
| title_short | Genetic and epigenetic control of gene expression by CRISPR–Cas systems [version 1; referees: 3 approved] |
| title_sort | genetic and epigenetic control of gene expression by crispr cas systems version 1 referees 3 approved |
| topic | Biocatalysis Chemical Biology of the Cell Control of Gene Expression Genomics Microbial Evolution & Genomics Microbial Physiology & Metabolism Plant Genomes & Evolution Protein Chemistry & Proteomics |
| url | https://f1000research.com/articles/6-747/v1 |
| work_keys_str_mv | AT albertlo geneticandepigeneticcontrolofgeneexpressionbycrisprcassystemsversion1referees3approved AT leiqi geneticandepigeneticcontrolofgeneexpressionbycrisprcassystemsversion1referees3approved |