Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models
In recent decades, cell biology has made rapid progress. Cell isolation and cultivation techniques, supported by modern laboratory procedures and experimental capabilities, provide a wide range of opportunities for in vitro research to study physiological and pathophysiological processes in health a...
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MDPI AG
2021-11-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/14/22/6857 |
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| author | Lidija Gradišnik Roman Bošnjak Gorazd Bunc Janez Ravnik Tina Maver Tomaž Velnar |
| author_facet | Lidija Gradišnik Roman Bošnjak Gorazd Bunc Janez Ravnik Tina Maver Tomaž Velnar |
| author_sort | Lidija Gradišnik |
| collection | DOAJ |
| description | In recent decades, cell biology has made rapid progress. Cell isolation and cultivation techniques, supported by modern laboratory procedures and experimental capabilities, provide a wide range of opportunities for in vitro research to study physiological and pathophysiological processes in health and disease. They can also be used very efficiently for the analysis of biomaterials. Before a new biomaterial is ready for implantation into tissues and widespread use in clinical practice, it must be extensively tested. Experimental cell models, which are a suitable testing ground and the first line of empirical exploration of new biomaterials, must contain suitable cells that form the basis of biomaterial testing. To isolate a stable and suitable cell culture, many steps are required. The first and one of the most important steps is the collection of donor tissue, usually during a surgical procedure. Thus, the collection is the foundation for the success of cell isolation. This article explains the sources and neurosurgical procedures for obtaining brain tissue samples for cell isolation techniques, which are essential for biomaterial testing procedures. |
| first_indexed | 2024-03-10T05:20:01Z |
| format | Article |
| id | doaj.art-b8e81d68b99a47c78766142815129b4a |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T05:20:01Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-b8e81d68b99a47c78766142815129b4a2023-11-23T00:09:53ZengMDPI AGMaterials1996-19442021-11-011422685710.3390/ma14226857Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell ModelsLidija Gradišnik0Roman Bošnjak1Gorazd Bunc2Janez Ravnik3Tina Maver4Tomaž Velnar5Faculty of Medicine, Institute of Biomedical Sciences, University of Maribor, Taborska 8, 2000 Maribor, SloveniaDepartment of Neurosurgery, University Medical Centre Ljubljana, Zaloska 7, 1000 Ljubljana, SloveniaDepartment of Neurosurgery, University Medical Centre Maribor, Ljubljanska 5, 2000 Maribor, SloveniaDepartment of Neurosurgery, University Medical Centre Maribor, Ljubljanska 5, 2000 Maribor, SloveniaFaculty of Medicine, Institute of Biomedical Sciences, University of Maribor, Taborska 8, 2000 Maribor, SloveniaAlma Mater Europaea ECM, Slovenska 17, 2000 Maribor, SloveniaIn recent decades, cell biology has made rapid progress. Cell isolation and cultivation techniques, supported by modern laboratory procedures and experimental capabilities, provide a wide range of opportunities for in vitro research to study physiological and pathophysiological processes in health and disease. They can also be used very efficiently for the analysis of biomaterials. Before a new biomaterial is ready for implantation into tissues and widespread use in clinical practice, it must be extensively tested. Experimental cell models, which are a suitable testing ground and the first line of empirical exploration of new biomaterials, must contain suitable cells that form the basis of biomaterial testing. To isolate a stable and suitable cell culture, many steps are required. The first and one of the most important steps is the collection of donor tissue, usually during a surgical procedure. Thus, the collection is the foundation for the success of cell isolation. This article explains the sources and neurosurgical procedures for obtaining brain tissue samples for cell isolation techniques, which are essential for biomaterial testing procedures.https://www.mdpi.com/1996-1944/14/22/6857cell isolationbrainexperimental cell modelsbiomaterial testingneurosurgery |
| spellingShingle | Lidija Gradišnik Roman Bošnjak Gorazd Bunc Janez Ravnik Tina Maver Tomaž Velnar Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models Materials cell isolation brain experimental cell models biomaterial testing neurosurgery |
| title | Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models |
| title_full | Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models |
| title_fullStr | Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models |
| title_full_unstemmed | Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models |
| title_short | Neurosurgical Approaches to Brain Tissue Harvesting for the Establishment of Cell Cultures in Neural Experimental Cell Models |
| title_sort | neurosurgical approaches to brain tissue harvesting for the establishment of cell cultures in neural experimental cell models |
| topic | cell isolation brain experimental cell models biomaterial testing neurosurgery |
| url | https://www.mdpi.com/1996-1944/14/22/6857 |
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