Preliminary exploration of the effects of environmental factors on the microsatellite status of BRAF-mutated colorectal cancer
Abstract Background To investigate the expression of EBV products and frequency of gallstone disease (GD) among different microsatellite status in colorectal cancer (CRC) with BRAFV600E mutation. Methods We collected 30 CRC patients with BRAFV600E mutation and 10 BRAF ( −) CRC patients as well as 54...
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | World Journal of Surgical Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12957-023-03106-6 |
| _version_ | 1797452469147533312 |
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| author | Binle Tian Guiming Chen Xiaoqin Shi Liren Jiang Tao Jiang Qi Li Lin Yuan Jian Qin |
| author_facet | Binle Tian Guiming Chen Xiaoqin Shi Liren Jiang Tao Jiang Qi Li Lin Yuan Jian Qin |
| author_sort | Binle Tian |
| collection | DOAJ |
| description | Abstract Background To investigate the expression of EBV products and frequency of gallstone disease (GD) among different microsatellite status in colorectal cancer (CRC) with BRAFV600E mutation. Methods We collected 30 CRC patients with BRAFV600E mutation and 10 BRAF ( −) CRC patients as well as 54 healthy subjects. Tumor tissue samples were collected to detect the mutation of BRAF, KRAS, and TP53. Microsatellite status was determined by immunohistochemistry and PCR. EBER in situ hybridization was performed to detect EBV. In addition, we also collected clinical information about the patients. Results We found that although EBV products were detected in CRC, there were no significant differences in the EBV distribution between the different BRAF groups. Our study demonstrated that BRAFV600E mutation and BRAFV600E with MSI were significantly more frequent in the right CRC. Furthermore, the KRAS mutation rate in the BRAF-wild-type group was proved to be significantly higher than that in the BRAF mutation group. In addition, we revealed that BRAF mutation and MSI were independent risk factors of TNM stage. The frequency of GD was higher in CRC patients than in general population, and although there was no significant difference between CRC with or without BRAFV600E mutation, the highest frequency of GD was found in MSS CRC with BRAFV600E mutation. Conclusions EBV plays a role in CRC, but is not a determinant of different microsatellite status in CRC with BRAFV600E mutation. The frequency of GD in MSS CRC with BRAFV600E mutation is significantly higher than that in the general population. |
| first_indexed | 2024-03-09T15:09:08Z |
| format | Article |
| id | doaj.art-b8e85fa723864947af856fc34d7d37bc |
| institution | Directory Open Access Journal |
| issn | 1477-7819 |
| language | English |
| last_indexed | 2024-03-09T15:09:08Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj.art-b8e85fa723864947af856fc34d7d37bc2023-11-26T13:31:06ZengBMCWorld Journal of Surgical Oncology1477-78192023-08-0121111110.1186/s12957-023-03106-6Preliminary exploration of the effects of environmental factors on the microsatellite status of BRAF-mutated colorectal cancerBinle Tian0Guiming Chen1Xiaoqin Shi2Liren Jiang3Tao Jiang4Qi Li5Lin Yuan6Jian Qin7Department of Oncology, Shanghai General Hospital, Shanghai Jiao Tong University School of MedicineDepartment of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of MedicinePathology Center, Shanghai General Hospital, Shanghai Jiao Tong University School of MedicinePathology Center, Shanghai General Hospital, Shanghai Jiao Tong University School of MedicineDepartment of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Oncology, Shanghai General Hospital, Shanghai Jiao Tong University School of MedicinePathology Center, Shanghai General Hospital, Shanghai Jiao Tong University School of MedicineDepartment of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of MedicineAbstract Background To investigate the expression of EBV products and frequency of gallstone disease (GD) among different microsatellite status in colorectal cancer (CRC) with BRAFV600E mutation. Methods We collected 30 CRC patients with BRAFV600E mutation and 10 BRAF ( −) CRC patients as well as 54 healthy subjects. Tumor tissue samples were collected to detect the mutation of BRAF, KRAS, and TP53. Microsatellite status was determined by immunohistochemistry and PCR. EBER in situ hybridization was performed to detect EBV. In addition, we also collected clinical information about the patients. Results We found that although EBV products were detected in CRC, there were no significant differences in the EBV distribution between the different BRAF groups. Our study demonstrated that BRAFV600E mutation and BRAFV600E with MSI were significantly more frequent in the right CRC. Furthermore, the KRAS mutation rate in the BRAF-wild-type group was proved to be significantly higher than that in the BRAF mutation group. In addition, we revealed that BRAF mutation and MSI were independent risk factors of TNM stage. The frequency of GD was higher in CRC patients than in general population, and although there was no significant difference between CRC with or without BRAFV600E mutation, the highest frequency of GD was found in MSS CRC with BRAFV600E mutation. Conclusions EBV plays a role in CRC, but is not a determinant of different microsatellite status in CRC with BRAFV600E mutation. The frequency of GD in MSS CRC with BRAFV600E mutation is significantly higher than that in the general population.https://doi.org/10.1186/s12957-023-03106-6Colorectal cancerBRAFMicrosatellite statusEBVGallstone disease |
| spellingShingle | Binle Tian Guiming Chen Xiaoqin Shi Liren Jiang Tao Jiang Qi Li Lin Yuan Jian Qin Preliminary exploration of the effects of environmental factors on the microsatellite status of BRAF-mutated colorectal cancer World Journal of Surgical Oncology Colorectal cancer BRAF Microsatellite status EBV Gallstone disease |
| title | Preliminary exploration of the effects of environmental factors on the microsatellite status of BRAF-mutated colorectal cancer |
| title_full | Preliminary exploration of the effects of environmental factors on the microsatellite status of BRAF-mutated colorectal cancer |
| title_fullStr | Preliminary exploration of the effects of environmental factors on the microsatellite status of BRAF-mutated colorectal cancer |
| title_full_unstemmed | Preliminary exploration of the effects of environmental factors on the microsatellite status of BRAF-mutated colorectal cancer |
| title_short | Preliminary exploration of the effects of environmental factors on the microsatellite status of BRAF-mutated colorectal cancer |
| title_sort | preliminary exploration of the effects of environmental factors on the microsatellite status of braf mutated colorectal cancer |
| topic | Colorectal cancer BRAF Microsatellite status EBV Gallstone disease |
| url | https://doi.org/10.1186/s12957-023-03106-6 |
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