Biotic and Abiotic Elicitors of Stilbenes Production in <i>Vitis vinifera</i> L. Cell Culture
The in vitro cell cultures derived from the grapevine (<i>Vitis vinifera</i> L.) have been used for the production of stilbenes treated with different biotic and abiotic elicitors. The red-grape cultivar Váh has been elicited by natural cellulose from <i>Trichoderma viride</i>...
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MDPI AG
2021-03-01
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| Online Access: | https://www.mdpi.com/2223-7747/10/3/490 |
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| author | Martin Sák Ivana Dokupilová Šarlota Kaňuková Michaela Mrkvová Daniel Mihálik Pavol Hauptvogel Ján Kraic |
| author_facet | Martin Sák Ivana Dokupilová Šarlota Kaňuková Michaela Mrkvová Daniel Mihálik Pavol Hauptvogel Ján Kraic |
| author_sort | Martin Sák |
| collection | DOAJ |
| description | The in vitro cell cultures derived from the grapevine (<i>Vitis vinifera</i> L.) have been used for the production of stilbenes treated with different biotic and abiotic elicitors. The red-grape cultivar Váh has been elicited by natural cellulose from <i>Trichoderma viride</i>, the cell wall homogenate from <i>Fusarium oxysporum</i> and synthetic jasmonates. The sodium-orthovanadate, known as an inhibitor of hypersensitive necrotic response in treated plant cells able to enhance production and release of secondary metabolite into the cultivation medium, was used as an abiotic elicitor. Growth of cells and the content of phenolic compounds <i>trans</i>-resveratrol, <i>trans</i>-piceid, δ-viniferin, and ɛ-viniferin, were analyzed in grapevine cells treated by individual elicitors. The highest accumulation of analyzed individual stilbenes, except of trans-piceid has been observed after treatment with the cell wall homogenate from <i>F. oxysporum</i>. Maximum production of trans-resveratrol, δ- and ɛ-viniferins was triggered by treatment with cellulase from <i>T. viride</i>. The accumulation of trans-piceid in cell cultures elicited by this cellulase revealed exactly the opposite effect, with almost three times higher production of trans-resveratrol than that of <i>trans</i>-piceid. This study suggested that both used fungal elicitors can enhance production more effectively than commonly used jasmonates. |
| first_indexed | 2024-03-09T05:24:37Z |
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| language | English |
| last_indexed | 2024-03-09T05:24:37Z |
| publishDate | 2021-03-01 |
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| series | Plants |
| spelling | doaj.art-b8eb07d1b5a6487aadcd151bce94b3042023-12-03T12:37:56ZengMDPI AGPlants2223-77472021-03-0110349010.3390/plants10030490Biotic and Abiotic Elicitors of Stilbenes Production in <i>Vitis vinifera</i> L. Cell CultureMartin Sák0Ivana Dokupilová1Šarlota Kaňuková2Michaela Mrkvová3Daniel Mihálik4Pavol Hauptvogel5Ján Kraic6Department of Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Námestie J. Herdu 2, 91701 Trnava, SlovakiaLiqoil-Pharm s.r.o., Bratislavská 535, 90046 Most pri Bratislave, SlovakiaDepartment of Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Námestie J. Herdu 2, 91701 Trnava, SlovakiaDepartment of Biology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Námestie J. Herdu 2, 91701 Trnava, SlovakiaDepartment of Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Námestie J. Herdu 2, 91701 Trnava, SlovakiaResearch Institute of Plant Production, National Agricultural and Food Centre, Bratislavská cesta 122, 92101 Piešťany, SlovakiaDepartment of Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Námestie J. Herdu 2, 91701 Trnava, SlovakiaThe in vitro cell cultures derived from the grapevine (<i>Vitis vinifera</i> L.) have been used for the production of stilbenes treated with different biotic and abiotic elicitors. The red-grape cultivar Váh has been elicited by natural cellulose from <i>Trichoderma viride</i>, the cell wall homogenate from <i>Fusarium oxysporum</i> and synthetic jasmonates. The sodium-orthovanadate, known as an inhibitor of hypersensitive necrotic response in treated plant cells able to enhance production and release of secondary metabolite into the cultivation medium, was used as an abiotic elicitor. Growth of cells and the content of phenolic compounds <i>trans</i>-resveratrol, <i>trans</i>-piceid, δ-viniferin, and ɛ-viniferin, were analyzed in grapevine cells treated by individual elicitors. The highest accumulation of analyzed individual stilbenes, except of trans-piceid has been observed after treatment with the cell wall homogenate from <i>F. oxysporum</i>. Maximum production of trans-resveratrol, δ- and ɛ-viniferins was triggered by treatment with cellulase from <i>T. viride</i>. The accumulation of trans-piceid in cell cultures elicited by this cellulase revealed exactly the opposite effect, with almost three times higher production of trans-resveratrol than that of <i>trans</i>-piceid. This study suggested that both used fungal elicitors can enhance production more effectively than commonly used jasmonates.https://www.mdpi.com/2223-7747/10/3/490<i>Vitis vinifera</i>cell cultureelicitationfungal elicitorsstilbenes |
| spellingShingle | Martin Sák Ivana Dokupilová Šarlota Kaňuková Michaela Mrkvová Daniel Mihálik Pavol Hauptvogel Ján Kraic Biotic and Abiotic Elicitors of Stilbenes Production in <i>Vitis vinifera</i> L. Cell Culture Plants <i>Vitis vinifera</i> cell culture elicitation fungal elicitors stilbenes |
| title | Biotic and Abiotic Elicitors of Stilbenes Production in <i>Vitis vinifera</i> L. Cell Culture |
| title_full | Biotic and Abiotic Elicitors of Stilbenes Production in <i>Vitis vinifera</i> L. Cell Culture |
| title_fullStr | Biotic and Abiotic Elicitors of Stilbenes Production in <i>Vitis vinifera</i> L. Cell Culture |
| title_full_unstemmed | Biotic and Abiotic Elicitors of Stilbenes Production in <i>Vitis vinifera</i> L. Cell Culture |
| title_short | Biotic and Abiotic Elicitors of Stilbenes Production in <i>Vitis vinifera</i> L. Cell Culture |
| title_sort | biotic and abiotic elicitors of stilbenes production in i vitis vinifera i l cell culture |
| topic | <i>Vitis vinifera</i> cell culture elicitation fungal elicitors stilbenes |
| url | https://www.mdpi.com/2223-7747/10/3/490 |
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