Whole blood transfusion improves vascular integrity and increases survival in artemether-treated experimental cerebral malaria
Abstract Pathological features observed in both human and experimental cerebral malaria (ECM) are endothelial dysfunction and changes in blood components. Blood transfusion has been routinely used in patients with severe malarial anemia and can also benefit comatose and acidotic malaria patients. In...
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| Format: | Article |
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Nature Portfolio
2021-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-91499-3 |
| _version_ | 1818736199590739968 |
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| author | Saba Gul Flavia L. Ribeiro-Gomes Aline S. Moreira Guilherme S. Sanches Fabiana G. Conceição Cláudio Tadeu Daniel-Ribeiro Hans C. Ackerman Leonardo J. M. Carvalho |
| author_facet | Saba Gul Flavia L. Ribeiro-Gomes Aline S. Moreira Guilherme S. Sanches Fabiana G. Conceição Cláudio Tadeu Daniel-Ribeiro Hans C. Ackerman Leonardo J. M. Carvalho |
| author_sort | Saba Gul |
| collection | DOAJ |
| description | Abstract Pathological features observed in both human and experimental cerebral malaria (ECM) are endothelial dysfunction and changes in blood components. Blood transfusion has been routinely used in patients with severe malarial anemia and can also benefit comatose and acidotic malaria patients. In the present study Plasmodium berghei-infected mice were transfused intraperitoneally with 200 μL of whole blood along with 20 mg/kg of artemether. ECM mice showed severe thrombocytopenia and decreases in hematocrit. Artemether treatment markedly aggravated anemia within 24 h. Whole blood administration significantly prevented further drop in hematocrit and partially restored the platelet count. Increased levels of plasma angiopoietin-2 (Ang-2) remained high 24 h after artemether treatment but returned to normal levels 24 h after blood transfusion, indicating reversal to quiescence. Ang-1 was depleted in ECM mice and levels were not restored by any treatment. Blood transfusion prevented the aggravation of the breakdown of blood brain barrier after artemether treatment and decreased spleen congestion without affecting splenic lymphocyte populations. Critically, blood transfusion resulted in markedly improved survival of mice with ECM (75.9% compared to 50.9% receiving artemether only). These findings indicate that whole blood transfusion can be an effective adjuvant therapy for cerebral malaria. |
| first_indexed | 2024-12-18T00:33:21Z |
| format | Article |
| id | doaj.art-b8ef0055519d46e8a9563dd4cc95860d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T00:33:21Z |
| publishDate | 2021-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b8ef0055519d46e8a9563dd4cc95860d2022-12-21T21:27:05ZengNature PortfolioScientific Reports2045-23222021-06-0111111310.1038/s41598-021-91499-3Whole blood transfusion improves vascular integrity and increases survival in artemether-treated experimental cerebral malariaSaba Gul0Flavia L. Ribeiro-Gomes1Aline S. Moreira2Guilherme S. Sanches3Fabiana G. Conceição4Cláudio Tadeu Daniel-Ribeiro5Hans C. Ackerman6Leonardo J. M. Carvalho7Laboratory of Malaria Research, Oswaldo Cruz InstituteLaboratory of Malaria Research, Oswaldo Cruz InstituteLaboratory of Malaria Research, Oswaldo Cruz InstituteLaboratory of Malaria Research, Oswaldo Cruz InstituteLaboratory of Malaria Research, Oswaldo Cruz InstituteLaboratory of Malaria Research, Oswaldo Cruz InstituteLaboratory of Malaria and Vector Research, NIAID, NIHLaboratory of Malaria Research, Oswaldo Cruz InstituteAbstract Pathological features observed in both human and experimental cerebral malaria (ECM) are endothelial dysfunction and changes in blood components. Blood transfusion has been routinely used in patients with severe malarial anemia and can also benefit comatose and acidotic malaria patients. In the present study Plasmodium berghei-infected mice were transfused intraperitoneally with 200 μL of whole blood along with 20 mg/kg of artemether. ECM mice showed severe thrombocytopenia and decreases in hematocrit. Artemether treatment markedly aggravated anemia within 24 h. Whole blood administration significantly prevented further drop in hematocrit and partially restored the platelet count. Increased levels of plasma angiopoietin-2 (Ang-2) remained high 24 h after artemether treatment but returned to normal levels 24 h after blood transfusion, indicating reversal to quiescence. Ang-1 was depleted in ECM mice and levels were not restored by any treatment. Blood transfusion prevented the aggravation of the breakdown of blood brain barrier after artemether treatment and decreased spleen congestion without affecting splenic lymphocyte populations. Critically, blood transfusion resulted in markedly improved survival of mice with ECM (75.9% compared to 50.9% receiving artemether only). These findings indicate that whole blood transfusion can be an effective adjuvant therapy for cerebral malaria.https://doi.org/10.1038/s41598-021-91499-3 |
| spellingShingle | Saba Gul Flavia L. Ribeiro-Gomes Aline S. Moreira Guilherme S. Sanches Fabiana G. Conceição Cláudio Tadeu Daniel-Ribeiro Hans C. Ackerman Leonardo J. M. Carvalho Whole blood transfusion improves vascular integrity and increases survival in artemether-treated experimental cerebral malaria Scientific Reports |
| title | Whole blood transfusion improves vascular integrity and increases survival in artemether-treated experimental cerebral malaria |
| title_full | Whole blood transfusion improves vascular integrity and increases survival in artemether-treated experimental cerebral malaria |
| title_fullStr | Whole blood transfusion improves vascular integrity and increases survival in artemether-treated experimental cerebral malaria |
| title_full_unstemmed | Whole blood transfusion improves vascular integrity and increases survival in artemether-treated experimental cerebral malaria |
| title_short | Whole blood transfusion improves vascular integrity and increases survival in artemether-treated experimental cerebral malaria |
| title_sort | whole blood transfusion improves vascular integrity and increases survival in artemether treated experimental cerebral malaria |
| url | https://doi.org/10.1038/s41598-021-91499-3 |
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