Relationship of LEP, LEPR Variants, and LEP Methylation with Multiple Myeloma and Prognosis
Introduction: Leptin (LEP) and LEP receptor (LEPR) play roles in cancer progression. We evaluated LEP-2548G/A and LEPR 668 A/G variants in patients with multiple myeloma (MM). In addition, the methylation status of CpG sites at 31 and 51 nucleotides (nt) according to the transcription start region o...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Galenos Yayinevi
2024-02-01
|
| Series: | İstanbul Medical Journal |
| Subjects: | |
| Online Access: |
http://istanbulmedicaljournal.org/archives/archive-detail/article-preview/relationship-of-ilep-lepr-i-variants-and-ilep-i-me/64512
|
| _version_ | 1797293883981299712 |
|---|---|
| author | Yasemin Oyacı Ayşe Feyda Nursal İstemi Serin Sacide Pehlivan Mustafa Pehlivan |
| author_facet | Yasemin Oyacı Ayşe Feyda Nursal İstemi Serin Sacide Pehlivan Mustafa Pehlivan |
| author_sort | Yasemin Oyacı |
| collection | DOAJ |
| description | Introduction: Leptin (LEP) and LEP receptor (LEPR) play roles in cancer progression. We evaluated LEP-2548G/A and LEPR 668 A/G variants in patients with multiple myeloma (MM). In addition, the methylation status of CpG sites at 31 and 51 nucleotides (nt) according to the transcription start region of the LEPgene was examined. Methods: DNA was extracted from the peripheral blood of study participants who were healthy controls and patients with MM. These variants were analyzed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The methylation at -31 and -51 nt in the LEPwas performed using the methylation-specific PCR method. The 2-year progression-free survival (PFS) and 2-year overall survival (OS) were evaluated according to prognostic factors. Results: There was no significant difference in the genotype distributions of LEPR 668A/G and LEP-2548G/A between the control and patient groups (p>0.05). We found that -31 and -51 nt regions of the LEP gene were unmethylated in the patient group compared with the control group (p=0.051 and p=0.001, respectively). The -31 nt methylation was unchanged in 15 patients (78.94%). PFS and OS were higher in these patients than in the others. In multivariate analysis, the methylated/unmethylated ratio at -31 nt methylation was associated with a poor prognosis (p=0.020). Conclusion: To our knowledge, our study is the first to examine these variants and their methylation status in Turkish patients with MM. Our results showed that LEP gene -31 nt unmethylation was associated with PFS and OS. These results need to be confirmed in different ethnic and larger sample groups. |
| first_indexed | 2024-03-07T21:22:16Z |
| format | Article |
| id | doaj.art-b8f085d48d7a4709913fcec5c2226c51 |
| institution | Directory Open Access Journal |
| issn | 2619-9793 2148-094X |
| language | English |
| last_indexed | 2024-03-07T21:22:16Z |
| publishDate | 2024-02-01 |
| publisher | Galenos Yayinevi |
| record_format | Article |
| series | İstanbul Medical Journal |
| spelling | doaj.art-b8f085d48d7a4709913fcec5c2226c512024-02-27T10:28:07ZengGalenos Yayineviİstanbul Medical Journal2619-97932148-094X2024-02-0125161110.4274/imj.galenos.2024.0936513049054Relationship of LEP, LEPR Variants, and LEP Methylation with Multiple Myeloma and PrognosisYasemin Oyacı0Ayşe Feyda Nursal1İstemi Serin2Sacide Pehlivan3Mustafa Pehlivan4 İstanbul University Institute of Graduate Studies in Health Sciences, İstanbul, Turkey Hitit University Faculty of Medicine, Department of Medical Genetics, Çorum, Turkey Ağrı İbrahim Çeçen University, Ağrı Training and Research Hospital, Clinic of Hematology, Ağrı, Turkey İstanbul University, İstanbul Faculty of Medicine, Department of Medical Biology, İstanbul, Turkey University of Health Sciences Turkey, Başakşehir Çam and Sakura City Hospital, Clinic of Hematology, İstanbul, Turkey Introduction: Leptin (LEP) and LEP receptor (LEPR) play roles in cancer progression. We evaluated LEP-2548G/A and LEPR 668 A/G variants in patients with multiple myeloma (MM). In addition, the methylation status of CpG sites at 31 and 51 nucleotides (nt) according to the transcription start region of the LEPgene was examined. Methods: DNA was extracted from the peripheral blood of study participants who were healthy controls and patients with MM. These variants were analyzed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The methylation at -31 and -51 nt in the LEPwas performed using the methylation-specific PCR method. The 2-year progression-free survival (PFS) and 2-year overall survival (OS) were evaluated according to prognostic factors. Results: There was no significant difference in the genotype distributions of LEPR 668A/G and LEP-2548G/A between the control and patient groups (p>0.05). We found that -31 and -51 nt regions of the LEP gene were unmethylated in the patient group compared with the control group (p=0.051 and p=0.001, respectively). The -31 nt methylation was unchanged in 15 patients (78.94%). PFS and OS were higher in these patients than in the others. In multivariate analysis, the methylated/unmethylated ratio at -31 nt methylation was associated with a poor prognosis (p=0.020). Conclusion: To our knowledge, our study is the first to examine these variants and their methylation status in Turkish patients with MM. Our results showed that LEP gene -31 nt unmethylation was associated with PFS and OS. These results need to be confirmed in different ethnic and larger sample groups. http://istanbulmedicaljournal.org/archives/archive-detail/article-preview/relationship-of-ilep-lepr-i-variants-and-ilep-i-me/64512 multiple myelomaleptinleptin receptorpcr-rflpms-pcr |
| spellingShingle | Yasemin Oyacı Ayşe Feyda Nursal İstemi Serin Sacide Pehlivan Mustafa Pehlivan Relationship of LEP, LEPR Variants, and LEP Methylation with Multiple Myeloma and Prognosis İstanbul Medical Journal multiple myeloma leptin leptin receptor pcr-rflp ms-pcr |
| title | Relationship of LEP, LEPR Variants, and LEP Methylation with Multiple Myeloma and Prognosis |
| title_full | Relationship of LEP, LEPR Variants, and LEP Methylation with Multiple Myeloma and Prognosis |
| title_fullStr | Relationship of LEP, LEPR Variants, and LEP Methylation with Multiple Myeloma and Prognosis |
| title_full_unstemmed | Relationship of LEP, LEPR Variants, and LEP Methylation with Multiple Myeloma and Prognosis |
| title_short | Relationship of LEP, LEPR Variants, and LEP Methylation with Multiple Myeloma and Prognosis |
| title_sort | relationship of lep lepr variants and lep methylation with multiple myeloma and prognosis |
| topic | multiple myeloma leptin leptin receptor pcr-rflp ms-pcr |
| url |
http://istanbulmedicaljournal.org/archives/archive-detail/article-preview/relationship-of-ilep-lepr-i-variants-and-ilep-i-me/64512
|
| work_keys_str_mv | AT yaseminoyacı relationshipoflepleprvariantsandlepmethylationwithmultiplemyelomaandprognosis AT aysefeydanursal relationshipoflepleprvariantsandlepmethylationwithmultiplemyelomaandprognosis AT istemiserin relationshipoflepleprvariantsandlepmethylationwithmultiplemyelomaandprognosis AT sacidepehlivan relationshipoflepleprvariantsandlepmethylationwithmultiplemyelomaandprognosis AT mustafapehlivan relationshipoflepleprvariantsandlepmethylationwithmultiplemyelomaandprognosis |