Accuracy of the Zika IgM Antibody Capture Enzyme-Linked Immunosorbent Assay from the Centers for Disease Control and Prevention (CDC Zika MAC-ELISA) for Diagnosis of Zika Virus Infection
Serological diagnosis of Zika virus (ZIKV) infection is challenging because of antigenic cross-reactivity with dengue virus (DENV). This study evaluated the accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay (CDC Zika IgM MAC-ELISA) in differentiating between ZIKV and DENV i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/10/10/835 |
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| author | Moyra Machado Portilho Laise de Moraes Mariana Kikuti Leile Camila Jacob Nascimento Mitermayer Galvão Reis Viviane Sampaio Boaventura Ricardo Khouri Guilherme Sousa Ribeiro |
| author_facet | Moyra Machado Portilho Laise de Moraes Mariana Kikuti Leile Camila Jacob Nascimento Mitermayer Galvão Reis Viviane Sampaio Boaventura Ricardo Khouri Guilherme Sousa Ribeiro |
| author_sort | Moyra Machado Portilho |
| collection | DOAJ |
| description | Serological diagnosis of Zika virus (ZIKV) infection is challenging because of antigenic cross-reactivity with dengue virus (DENV). This study evaluated the accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay (CDC Zika IgM MAC-ELISA) in differentiating between ZIKV and DENV infections. To determine sensitivity, we used acute- and convalescent-phase sera from 21 patients with RT-PCR-confirmed ZIKV infection. To determine specificity, we used acute- and convalescent-phase sera from 60 RT-PCR-confirmed dengue cases and sera from 23 blood donors. During the acute-phase of the illness, the assay presented a sensitivity of 12.5% (2/16) for samples collected 0–4 days post symptoms onset (DPSO), and of 75.0% (3/4) for samples collected 5–9 DPSO. During the convalescent-phase of the illness, the test sensitivity was 90.9% (10/11), 100% (2/2), and 0% (0/2) for samples obtained 12–102, 258–260, and 722–727 DPSO, respectively. Specificity for acute- and convalescent-phase samples from RT-PCR-confirmed dengue cases was 100% and 93.2%, respectively. Specificity for blood donor samples was 100%. The assay is an accurate method for Zika serological diagnosis and proved to be reliable for use during surveillance and outbreak investigations in settings where ZIKV and DENV cocirculate. |
| first_indexed | 2024-03-10T15:32:47Z |
| format | Article |
| id | doaj.art-b8f21691d232496fad622e301630bd18 |
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| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T15:32:47Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-b8f21691d232496fad622e301630bd182023-11-20T17:32:32ZengMDPI AGDiagnostics2075-44182020-10-01101083510.3390/diagnostics10100835Accuracy of the Zika IgM Antibody Capture Enzyme-Linked Immunosorbent Assay from the Centers for Disease Control and Prevention (CDC Zika MAC-ELISA) for Diagnosis of Zika Virus InfectionMoyra Machado Portilho0Laise de Moraes1Mariana Kikuti2Leile Camila Jacob Nascimento3Mitermayer Galvão Reis4Viviane Sampaio Boaventura5Ricardo Khouri6Guilherme Sousa Ribeiro7Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BA 40296-710, BrazilInstituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BA 40296-710, BrazilInstituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BA 40296-710, BrazilInstituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BA 40296-710, BrazilInstituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BA 40296-710, BrazilInstituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BA 40296-710, BrazilInstituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BA 40296-710, BrazilInstituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BA 40296-710, BrazilSerological diagnosis of Zika virus (ZIKV) infection is challenging because of antigenic cross-reactivity with dengue virus (DENV). This study evaluated the accuracy of the Zika IgM antibody capture enzyme-linked immunosorbent assay (CDC Zika IgM MAC-ELISA) in differentiating between ZIKV and DENV infections. To determine sensitivity, we used acute- and convalescent-phase sera from 21 patients with RT-PCR-confirmed ZIKV infection. To determine specificity, we used acute- and convalescent-phase sera from 60 RT-PCR-confirmed dengue cases and sera from 23 blood donors. During the acute-phase of the illness, the assay presented a sensitivity of 12.5% (2/16) for samples collected 0–4 days post symptoms onset (DPSO), and of 75.0% (3/4) for samples collected 5–9 DPSO. During the convalescent-phase of the illness, the test sensitivity was 90.9% (10/11), 100% (2/2), and 0% (0/2) for samples obtained 12–102, 258–260, and 722–727 DPSO, respectively. Specificity for acute- and convalescent-phase samples from RT-PCR-confirmed dengue cases was 100% and 93.2%, respectively. Specificity for blood donor samples was 100%. The assay is an accurate method for Zika serological diagnosis and proved to be reliable for use during surveillance and outbreak investigations in settings where ZIKV and DENV cocirculate.https://www.mdpi.com/2075-4418/10/10/835Zika virusdiagnostic accuracysensitivity and specificityenzyme-linked immunosorbent assayCDC Zika MAC-ELISA |
| spellingShingle | Moyra Machado Portilho Laise de Moraes Mariana Kikuti Leile Camila Jacob Nascimento Mitermayer Galvão Reis Viviane Sampaio Boaventura Ricardo Khouri Guilherme Sousa Ribeiro Accuracy of the Zika IgM Antibody Capture Enzyme-Linked Immunosorbent Assay from the Centers for Disease Control and Prevention (CDC Zika MAC-ELISA) for Diagnosis of Zika Virus Infection Diagnostics Zika virus diagnostic accuracy sensitivity and specificity enzyme-linked immunosorbent assay CDC Zika MAC-ELISA |
| title | Accuracy of the Zika IgM Antibody Capture Enzyme-Linked Immunosorbent Assay from the Centers for Disease Control and Prevention (CDC Zika MAC-ELISA) for Diagnosis of Zika Virus Infection |
| title_full | Accuracy of the Zika IgM Antibody Capture Enzyme-Linked Immunosorbent Assay from the Centers for Disease Control and Prevention (CDC Zika MAC-ELISA) for Diagnosis of Zika Virus Infection |
| title_fullStr | Accuracy of the Zika IgM Antibody Capture Enzyme-Linked Immunosorbent Assay from the Centers for Disease Control and Prevention (CDC Zika MAC-ELISA) for Diagnosis of Zika Virus Infection |
| title_full_unstemmed | Accuracy of the Zika IgM Antibody Capture Enzyme-Linked Immunosorbent Assay from the Centers for Disease Control and Prevention (CDC Zika MAC-ELISA) for Diagnosis of Zika Virus Infection |
| title_short | Accuracy of the Zika IgM Antibody Capture Enzyme-Linked Immunosorbent Assay from the Centers for Disease Control and Prevention (CDC Zika MAC-ELISA) for Diagnosis of Zika Virus Infection |
| title_sort | accuracy of the zika igm antibody capture enzyme linked immunosorbent assay from the centers for disease control and prevention cdc zika mac elisa for diagnosis of zika virus infection |
| topic | Zika virus diagnostic accuracy sensitivity and specificity enzyme-linked immunosorbent assay CDC Zika MAC-ELISA |
| url | https://www.mdpi.com/2075-4418/10/10/835 |
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