Genotyping by Sequencing (GBS)-Based QTL Mapping for Bacterial Fruit Blotch (BFB) in Watermelon
Watermelon (<i>Citrullus lanatus</i>), an economically important and nutritionally rich Cucurbitaceous crop grown worldwide, is severely affected by bacterial fruit blotch (BFB). Development of resistant cultivar is the most eco-friendly, cost-effective, and sustainable way to tackle thi...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/12/2250 |
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| author | Sang-Min Yeo Jeongeui Hong Mohammad Rashed Hossain Hee-Jeong Jung Phillip Choe Ill-Sup Nou |
| author_facet | Sang-Min Yeo Jeongeui Hong Mohammad Rashed Hossain Hee-Jeong Jung Phillip Choe Ill-Sup Nou |
| author_sort | Sang-Min Yeo |
| collection | DOAJ |
| description | Watermelon (<i>Citrullus lanatus</i>), an economically important and nutritionally rich Cucurbitaceous crop grown worldwide, is severely affected by bacterial fruit blotch (BFB). Development of resistant cultivar is the most eco-friendly, cost-effective, and sustainable way to tackle this disease. This requires wider understanding of the genetics of resistance to BFB. In this study, we identified quantitative trait loci (QTLs) associated with BFB resistance in an F<sub>2</sub> mapping population developed from BFB-resistant ‘PI 189225’ (<i>Citrullus amarus</i>) and -susceptible ‘SW 26’ (<i>C. lanatus</i>) genotypes based on the polymorphic markers identified by genotyping by sequencing (GSB). A linkage map covering a total genetic distance of 3377.1 cM was constructed. Two QTLs for BFB resistance, namely, <i>ClBFB10.1</i> and <i>ClBFB10.2</i>, both located on chromosome 10 explaining 18.84 and 15.41% of the phenotypic variations, respectively, were identified. Two SNP-based high-resolution melting (HRM) markers <i>WmBFB10.1</i> and <i>WmBFB10.2</i> having high positive correlation with resistance vs. susceptible alleles were developed. The efficacy of the markers was validated in another F<sub>2</sub> population derived from SW34 × PI 189225. The highest phenotypic variation was found in the locus <i>ClBFB10.2</i>, which also contains three putative candidate genes for resistance to BFB. These findings will accelerate the development of BFB-resistant watermelon varieties via molecular breeding. |
| first_indexed | 2024-03-09T16:30:44Z |
| format | Article |
| id | doaj.art-b8f7d65b58594d0cbb2102060af0a9ff |
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| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T16:30:44Z |
| publishDate | 2022-11-01 |
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| record_format | Article |
| series | Genes |
| spelling | doaj.art-b8f7d65b58594d0cbb2102060af0a9ff2023-11-24T15:03:32ZengMDPI AGGenes2073-44252022-11-011312225010.3390/genes13122250Genotyping by Sequencing (GBS)-Based QTL Mapping for Bacterial Fruit Blotch (BFB) in WatermelonSang-Min Yeo0Jeongeui Hong1Mohammad Rashed Hossain2Hee-Jeong Jung3Phillip Choe4Ill-Sup Nou5Department of Horticulture, Sunchon National University, Suncheon 57922, Republic of KoreaDepartment of Biological Sciences and Biotechnology, Chungbuk National University, Cheongju 28644, Republic of KoreaDepartment of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Horticulture, Sunchon National University, Suncheon 57922, Republic of KoreaPPS Farming Corporation, Yongin 17096, Republic of KoreaDepartment of Horticulture, Sunchon National University, Suncheon 57922, Republic of KoreaWatermelon (<i>Citrullus lanatus</i>), an economically important and nutritionally rich Cucurbitaceous crop grown worldwide, is severely affected by bacterial fruit blotch (BFB). Development of resistant cultivar is the most eco-friendly, cost-effective, and sustainable way to tackle this disease. This requires wider understanding of the genetics of resistance to BFB. In this study, we identified quantitative trait loci (QTLs) associated with BFB resistance in an F<sub>2</sub> mapping population developed from BFB-resistant ‘PI 189225’ (<i>Citrullus amarus</i>) and -susceptible ‘SW 26’ (<i>C. lanatus</i>) genotypes based on the polymorphic markers identified by genotyping by sequencing (GSB). A linkage map covering a total genetic distance of 3377.1 cM was constructed. Two QTLs for BFB resistance, namely, <i>ClBFB10.1</i> and <i>ClBFB10.2</i>, both located on chromosome 10 explaining 18.84 and 15.41% of the phenotypic variations, respectively, were identified. Two SNP-based high-resolution melting (HRM) markers <i>WmBFB10.1</i> and <i>WmBFB10.2</i> having high positive correlation with resistance vs. susceptible alleles were developed. The efficacy of the markers was validated in another F<sub>2</sub> population derived from SW34 × PI 189225. The highest phenotypic variation was found in the locus <i>ClBFB10.2</i>, which also contains three putative candidate genes for resistance to BFB. These findings will accelerate the development of BFB-resistant watermelon varieties via molecular breeding.https://www.mdpi.com/2073-4425/13/12/2250bacterial fruit blotchquantitative trait locisingle nucleotide polymorphismhigh-resolution melting |
| spellingShingle | Sang-Min Yeo Jeongeui Hong Mohammad Rashed Hossain Hee-Jeong Jung Phillip Choe Ill-Sup Nou Genotyping by Sequencing (GBS)-Based QTL Mapping for Bacterial Fruit Blotch (BFB) in Watermelon Genes bacterial fruit blotch quantitative trait loci single nucleotide polymorphism high-resolution melting |
| title | Genotyping by Sequencing (GBS)-Based QTL Mapping for Bacterial Fruit Blotch (BFB) in Watermelon |
| title_full | Genotyping by Sequencing (GBS)-Based QTL Mapping for Bacterial Fruit Blotch (BFB) in Watermelon |
| title_fullStr | Genotyping by Sequencing (GBS)-Based QTL Mapping for Bacterial Fruit Blotch (BFB) in Watermelon |
| title_full_unstemmed | Genotyping by Sequencing (GBS)-Based QTL Mapping for Bacterial Fruit Blotch (BFB) in Watermelon |
| title_short | Genotyping by Sequencing (GBS)-Based QTL Mapping for Bacterial Fruit Blotch (BFB) in Watermelon |
| title_sort | genotyping by sequencing gbs based qtl mapping for bacterial fruit blotch bfb in watermelon |
| topic | bacterial fruit blotch quantitative trait loci single nucleotide polymorphism high-resolution melting |
| url | https://www.mdpi.com/2073-4425/13/12/2250 |
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