Formaldehyde Detection by a Combination of Formaldehyde Dehydrogenase and Chitosan on a Sensor Based on an Organic Field-Effect Transistor
Formaldehyde is utilized for the preservation of materials due to its strong bactericidal effects. As formaldehyde is also a harmful substance that causes health hazards, the quantitative monitoring of formaldehyde in natural and living environments is desirable. For the rapid and easy detection of...
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MDPI AG
2019-07-01
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| Series: | Technologies |
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| Online Access: | https://www.mdpi.com/2227-7080/7/3/48 |
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| author | Kazuhiko Tsuchiya Hiroyuki Furusawa Ayako Nomura Hiroyuki Matsui Mizuhisa Nihei Shizuo Tokito |
| author_facet | Kazuhiko Tsuchiya Hiroyuki Furusawa Ayako Nomura Hiroyuki Matsui Mizuhisa Nihei Shizuo Tokito |
| author_sort | Kazuhiko Tsuchiya |
| collection | DOAJ |
| description | Formaldehyde is utilized for the preservation of materials due to its strong bactericidal effects. As formaldehyde is also a harmful substance that causes health hazards, the quantitative monitoring of formaldehyde in natural and living environments is desirable. For the rapid and easy detection of formaldehyde, in this study we applied an organic field-effect transistor (OFET)-based sensor that can function as a potentiometric device for electrochemical measurements. A polyion-complex gel of formaldehyde dehydrogenase (FDH) and chitosan (CT) was constructed on a gold electrode. When the FDH/CT gel-coated electrode was connected to an OFET device it could detect formaldehyde in an aqueous solution, in which the amino groups of chitosan would protonate during the enzymatic reaction. The limit of detection was calculated to be 3.1 µM (93 ppb), demonstrating the applicability of the film-type OFET sensor to environmental monitoring. |
| first_indexed | 2024-04-13T01:22:31Z |
| format | Article |
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| issn | 2227-7080 |
| language | English |
| last_indexed | 2024-04-13T01:22:31Z |
| publishDate | 2019-07-01 |
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| series | Technologies |
| spelling | doaj.art-b8f8058258aa4dd0a0550bc85b001bf52022-12-22T03:08:43ZengMDPI AGTechnologies2227-70802019-07-01734810.3390/technologies7030048technologies7030048Formaldehyde Detection by a Combination of Formaldehyde Dehydrogenase and Chitosan on a Sensor Based on an Organic Field-Effect TransistorKazuhiko Tsuchiya0Hiroyuki Furusawa1Ayako Nomura2Hiroyuki Matsui3Mizuhisa Nihei4Shizuo Tokito5Research Center for Organic Electronics (ROEL), Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, JapanResearch Center for Organic Electronics (ROEL), Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, JapanResearch Center for Organic Electronics (ROEL), Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, JapanResearch Center for Organic Electronics (ROEL), Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, JapanMaterial Application Laboratory, Japan Research Center, Huawei Technologies Japan K.K., 3-1 Kinko-cho, Kanagawa-ku, Yokohama, Kanagawa 221-0056, JapanResearch Center for Organic Electronics (ROEL), Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, JapanFormaldehyde is utilized for the preservation of materials due to its strong bactericidal effects. As formaldehyde is also a harmful substance that causes health hazards, the quantitative monitoring of formaldehyde in natural and living environments is desirable. For the rapid and easy detection of formaldehyde, in this study we applied an organic field-effect transistor (OFET)-based sensor that can function as a potentiometric device for electrochemical measurements. A polyion-complex gel of formaldehyde dehydrogenase (FDH) and chitosan (CT) was constructed on a gold electrode. When the FDH/CT gel-coated electrode was connected to an OFET device it could detect formaldehyde in an aqueous solution, in which the amino groups of chitosan would protonate during the enzymatic reaction. The limit of detection was calculated to be 3.1 µM (93 ppb), demonstrating the applicability of the film-type OFET sensor to environmental monitoring.https://www.mdpi.com/2227-7080/7/3/48chitosanformaldehyde dehydrogenase (FDH)formaldehyde detectionorganic field-effect transistor (OFET)polyion-complex gel |
| spellingShingle | Kazuhiko Tsuchiya Hiroyuki Furusawa Ayako Nomura Hiroyuki Matsui Mizuhisa Nihei Shizuo Tokito Formaldehyde Detection by a Combination of Formaldehyde Dehydrogenase and Chitosan on a Sensor Based on an Organic Field-Effect Transistor Technologies chitosan formaldehyde dehydrogenase (FDH) formaldehyde detection organic field-effect transistor (OFET) polyion-complex gel |
| title | Formaldehyde Detection by a Combination of Formaldehyde Dehydrogenase and Chitosan on a Sensor Based on an Organic Field-Effect Transistor |
| title_full | Formaldehyde Detection by a Combination of Formaldehyde Dehydrogenase and Chitosan on a Sensor Based on an Organic Field-Effect Transistor |
| title_fullStr | Formaldehyde Detection by a Combination of Formaldehyde Dehydrogenase and Chitosan on a Sensor Based on an Organic Field-Effect Transistor |
| title_full_unstemmed | Formaldehyde Detection by a Combination of Formaldehyde Dehydrogenase and Chitosan on a Sensor Based on an Organic Field-Effect Transistor |
| title_short | Formaldehyde Detection by a Combination of Formaldehyde Dehydrogenase and Chitosan on a Sensor Based on an Organic Field-Effect Transistor |
| title_sort | formaldehyde detection by a combination of formaldehyde dehydrogenase and chitosan on a sensor based on an organic field effect transistor |
| topic | chitosan formaldehyde dehydrogenase (FDH) formaldehyde detection organic field-effect transistor (OFET) polyion-complex gel |
| url | https://www.mdpi.com/2227-7080/7/3/48 |
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