Supplementation of cyanidin-3-O-β-glucoside-rich haskap (Lonicera caerulea L.) berry extract attenuates hepatic lipid dysregulation in diet-induced obese mice
Haskap (Lonicera caerulea L.) berry is enriched in anthocyanins, primarily cyanidin-3-O-β-glucoside (C3G). It remains unknown whether C3G counteracts metabolic alterations of the pathogenesis of obesity. In this study, mice were fed high-fat high-sucrose (HFHS) diet supplemented either with C3G-rich...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623002359 |
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| author | Dipsikha Biswas A.B.K.H. De Silva Angella Mercer Shreya Sarkar Petra Kienesberger Morgan Langille H.P.Vasantha Rupasinghe Thomas Pulinilkunnil |
| author_facet | Dipsikha Biswas A.B.K.H. De Silva Angella Mercer Shreya Sarkar Petra Kienesberger Morgan Langille H.P.Vasantha Rupasinghe Thomas Pulinilkunnil |
| author_sort | Dipsikha Biswas |
| collection | DOAJ |
| description | Haskap (Lonicera caerulea L.) berry is enriched in anthocyanins, primarily cyanidin-3-O-β-glucoside (C3G). It remains unknown whether C3G counteracts metabolic alterations of the pathogenesis of obesity. In this study, mice were fed high-fat high-sucrose (HFHS) diet supplemented either with C3G-rich extract (HFHS + CE) or berry powder with low C3G (HFHS + BP). Mice fed HFHS + CE displayed short-term protection against weight gain, independent of food intake. HFHS + CE mice had lower hepatic diacylglycerols and triacylglycerols content and reduced expression of key lipogenic transcription factors. These metabolic changes also translated into improved glucose tolerance and insulin sensitivity. 16S rRNA sequencing revealed altered gut microbiota composition in the HFHS + CE group. In summary, we demonstrate that C3G enrichment in the HFHS diet attenuates short-term weight gain, decreases hepatic lipid content by suppressing key lipogenic gene expression and improves glucose homeostasis during obesity development, supporting the therapeutic utility of C3G as a bioactive phytonutrient to manage obesity-related complications. |
| first_indexed | 2024-03-12T01:38:27Z |
| format | Article |
| id | doaj.art-b8f8f8a9ac454ef4a9c14026c0818e4a |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-12T01:38:27Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-b8f8f8a9ac454ef4a9c14026c0818e4a2023-09-11T04:16:44ZengElsevierJournal of Functional Foods1756-46462023-09-01108105635Supplementation of cyanidin-3-O-β-glucoside-rich haskap (Lonicera caerulea L.) berry extract attenuates hepatic lipid dysregulation in diet-induced obese miceDipsikha Biswas0A.B.K.H. De Silva1Angella Mercer2Shreya Sarkar3Petra Kienesberger4Morgan Langille5H.P.Vasantha Rupasinghe6Thomas Pulinilkunnil7Department of Biochemistry and Molecular Biology, Faculty of Medicine, Dalhousie University, Dalhousie Medicine New Brunswick, Saint John, New Brunswick, CanadaDepartment of Plant, Food, and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, Nova Scotia, CanadaDepartment of Biochemistry and Molecular Biology, Faculty of Medicine, Dalhousie University, Dalhousie Medicine New Brunswick, Saint John, New Brunswick, CanadaDepartment of Biochemistry and Molecular Biology, Faculty of Medicine, Dalhousie University, Dalhousie Medicine New Brunswick, Saint John, New Brunswick, CanadaDepartment of Biochemistry and Molecular Biology, Faculty of Medicine, Dalhousie University, Dalhousie Medicine New Brunswick, Saint John, New Brunswick, CanadaDepartment of Pharmacology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, CanadaDepartment of Plant, Food, and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, Nova Scotia, Canada; Department of Pathology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada; Corresponding authors at: Department of Biochemistry and Molecular Biology, Faculty of Medicine, Dalhousie University Dalhousie Medicine New Brunswick, 100 Tucker Park Road, Saint John E2L4L5, New Brunswick, Canada.Department of Biochemistry and Molecular Biology, Faculty of Medicine, Dalhousie University, Dalhousie Medicine New Brunswick, Saint John, New Brunswick, Canada; Corresponding authors at: Department of Biochemistry and Molecular Biology, Faculty of Medicine, Dalhousie University Dalhousie Medicine New Brunswick, 100 Tucker Park Road, Saint John E2L4L5, New Brunswick, Canada.Haskap (Lonicera caerulea L.) berry is enriched in anthocyanins, primarily cyanidin-3-O-β-glucoside (C3G). It remains unknown whether C3G counteracts metabolic alterations of the pathogenesis of obesity. In this study, mice were fed high-fat high-sucrose (HFHS) diet supplemented either with C3G-rich extract (HFHS + CE) or berry powder with low C3G (HFHS + BP). Mice fed HFHS + CE displayed short-term protection against weight gain, independent of food intake. HFHS + CE mice had lower hepatic diacylglycerols and triacylglycerols content and reduced expression of key lipogenic transcription factors. These metabolic changes also translated into improved glucose tolerance and insulin sensitivity. 16S rRNA sequencing revealed altered gut microbiota composition in the HFHS + CE group. In summary, we demonstrate that C3G enrichment in the HFHS diet attenuates short-term weight gain, decreases hepatic lipid content by suppressing key lipogenic gene expression and improves glucose homeostasis during obesity development, supporting the therapeutic utility of C3G as a bioactive phytonutrient to manage obesity-related complications.http://www.sciencedirect.com/science/article/pii/S1756464623002359ObesityDiabetesHaskapAnthocyaninCyanidinWestern diet |
| spellingShingle | Dipsikha Biswas A.B.K.H. De Silva Angella Mercer Shreya Sarkar Petra Kienesberger Morgan Langille H.P.Vasantha Rupasinghe Thomas Pulinilkunnil Supplementation of cyanidin-3-O-β-glucoside-rich haskap (Lonicera caerulea L.) berry extract attenuates hepatic lipid dysregulation in diet-induced obese mice Journal of Functional Foods Obesity Diabetes Haskap Anthocyanin Cyanidin Western diet |
| title | Supplementation of cyanidin-3-O-β-glucoside-rich haskap (Lonicera caerulea L.) berry extract attenuates hepatic lipid dysregulation in diet-induced obese mice |
| title_full | Supplementation of cyanidin-3-O-β-glucoside-rich haskap (Lonicera caerulea L.) berry extract attenuates hepatic lipid dysregulation in diet-induced obese mice |
| title_fullStr | Supplementation of cyanidin-3-O-β-glucoside-rich haskap (Lonicera caerulea L.) berry extract attenuates hepatic lipid dysregulation in diet-induced obese mice |
| title_full_unstemmed | Supplementation of cyanidin-3-O-β-glucoside-rich haskap (Lonicera caerulea L.) berry extract attenuates hepatic lipid dysregulation in diet-induced obese mice |
| title_short | Supplementation of cyanidin-3-O-β-glucoside-rich haskap (Lonicera caerulea L.) berry extract attenuates hepatic lipid dysregulation in diet-induced obese mice |
| title_sort | supplementation of cyanidin 3 o β glucoside rich haskap lonicera caerulea l berry extract attenuates hepatic lipid dysregulation in diet induced obese mice |
| topic | Obesity Diabetes Haskap Anthocyanin Cyanidin Western diet |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623002359 |
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