An Electrochemical Immunosensor Based on Carboxylated Graphene/SPCE for IgG-SARS-CoV-2 Nucleocapsid Determination
The COVID-19 pandemic has emphasized the importance and urgent need for rapid and accurate diagnostic tests for detecting and screening this infection. Our proposal was to develop a biosensor based on an ELISA immunoassay for monitoring antibodies against SARS-CoV-2 in human serum samples. The nucle...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/12/1161 |
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| author | Luciana de Souza Freire Camila Macena Ruzo Bárbara Batista Salgado Ariamna María Dip Gandarilla Yonny Romaguera-Barcelay Ana P. M. Tavares Maria Goreti Ferreira Sales Isabelle Cordeiro Jaila Dias Borges Lalwani Robert Matos Henrique Fonseca Filho Spartaco Astolfi-Filho Ştefan Ţălu Pritesh Lalwani Walter Ricardo Brito |
| author_facet | Luciana de Souza Freire Camila Macena Ruzo Bárbara Batista Salgado Ariamna María Dip Gandarilla Yonny Romaguera-Barcelay Ana P. M. Tavares Maria Goreti Ferreira Sales Isabelle Cordeiro Jaila Dias Borges Lalwani Robert Matos Henrique Fonseca Filho Spartaco Astolfi-Filho Ştefan Ţălu Pritesh Lalwani Walter Ricardo Brito |
| author_sort | Luciana de Souza Freire |
| collection | DOAJ |
| description | The COVID-19 pandemic has emphasized the importance and urgent need for rapid and accurate diagnostic tests for detecting and screening this infection. Our proposal was to develop a biosensor based on an ELISA immunoassay for monitoring antibodies against SARS-CoV-2 in human serum samples. The nucleocapsid protein (N protein) from SARS-CoV-2 was employed as a specific receptor for the detection of SARS-CoV-2 nucleocapsid immunoglobulin G. N protein was immobilized on the surface of a screen-printed carbon electrode (SPCE) modified with carboxylated graphene (CG). The percentage of IgG-SARS-CoV-2 nucleocapsid present was quantified using a secondary antibody labeled with horseradish peroxidase (HRP) (anti-IgG-HRP) catalyzed using 3,3′,5,5′-tetramethylbenzidine (TMB) mediator by chronoamperometry. A linear response was obtained in the range of 1:1000–1:200 <i>v/v</i> in phosphate buffer solution (PBS), and the detection limit calculated was 1:4947 <i>v</i>/<i>v</i>. The chronoamperometric method showed electrical signals directly proportional to antibody concentrations due to antigen-antibody (Ag-Ab) specific and stable binding reaction. |
| first_indexed | 2024-03-09T17:16:32Z |
| format | Article |
| id | doaj.art-b8fc4c2f86b44ea8a66a62f886f174c3 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T17:16:32Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-b8fc4c2f86b44ea8a66a62f886f174c32023-11-24T13:37:37ZengMDPI AGBiosensors2079-63742022-12-011212116110.3390/bios12121161An Electrochemical Immunosensor Based on Carboxylated Graphene/SPCE for IgG-SARS-CoV-2 Nucleocapsid DeterminationLuciana de Souza Freire0Camila Macena Ruzo1Bárbara Batista Salgado2Ariamna María Dip Gandarilla3Yonny Romaguera-Barcelay4Ana P. M. Tavares5Maria Goreti Ferreira Sales6Isabelle Cordeiro7Jaila Dias Borges Lalwani8Robert Matos9Henrique Fonseca Filho10Spartaco Astolfi-Filho11Ştefan Ţălu12Pritesh Lalwani13Walter Ricardo Brito14Department of Chemistry, Institute of Exact Sciences, Federal University of Amazonas, Manaus 69067-005, AM, BrazilDepartment of Chemistry, Institute of Exact Sciences, Federal University of Amazonas, Manaus 69067-005, AM, BrazilInstituto Leônidas e Maria Deane (ILMD), Fiocruz Amazônia, Manaus 69067-005, AM, BrazilDepartment of Chemistry, Institute of Exact Sciences, Federal University of Amazonas, Manaus 69067-005, AM, BrazilDepartment of Chemistry, Institute of Exact Sciences, Federal University of Amazonas, Manaus 69067-005, AM, BrazilBioMark@UC, Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, 3030-790 Coimbra, PortugalBioMark@UC, Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, 3030-790 Coimbra, PortugalDepartment of Physiological Sciences, Institute of Biological Sciences, Federal University of Amazonas, Manaus 69067-005, AM, BrazilFaculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus 69067-005, AM, BrazilAmazonian Materials Group, Federal University of Amapá (UNIFAP), Macapá 49100-000, AP, BrazilLaboratory of Nanomaterials Synthesis and Nanoscopy (LSNN), Federal University of Amazonas (UFAM), Manaus 69067-005, AM, BrazilDepartment of Chemistry, Institute of Exact Sciences, Federal University of Amazonas, Manaus 69067-005, AM, BrazilThe Directorate of Research, Development and Innovation Management (DMCDI), The Technical University of Cluj-Napoca, Constantin Daicoviciu Street, No. 15, 400020 Cluj-Napoca, RomaniaInstituto Leônidas e Maria Deane (ILMD), Fiocruz Amazônia, Manaus 69067-005, AM, BrazilDepartment of Chemistry, Institute of Exact Sciences, Federal University of Amazonas, Manaus 69067-005, AM, BrazilThe COVID-19 pandemic has emphasized the importance and urgent need for rapid and accurate diagnostic tests for detecting and screening this infection. Our proposal was to develop a biosensor based on an ELISA immunoassay for monitoring antibodies against SARS-CoV-2 in human serum samples. The nucleocapsid protein (N protein) from SARS-CoV-2 was employed as a specific receptor for the detection of SARS-CoV-2 nucleocapsid immunoglobulin G. N protein was immobilized on the surface of a screen-printed carbon electrode (SPCE) modified with carboxylated graphene (CG). The percentage of IgG-SARS-CoV-2 nucleocapsid present was quantified using a secondary antibody labeled with horseradish peroxidase (HRP) (anti-IgG-HRP) catalyzed using 3,3′,5,5′-tetramethylbenzidine (TMB) mediator by chronoamperometry. A linear response was obtained in the range of 1:1000–1:200 <i>v/v</i> in phosphate buffer solution (PBS), and the detection limit calculated was 1:4947 <i>v</i>/<i>v</i>. The chronoamperometric method showed electrical signals directly proportional to antibody concentrations due to antigen-antibody (Ag-Ab) specific and stable binding reaction.https://www.mdpi.com/2079-6374/12/12/1161immunosensorSARS-CoV-2N protein |
| spellingShingle | Luciana de Souza Freire Camila Macena Ruzo Bárbara Batista Salgado Ariamna María Dip Gandarilla Yonny Romaguera-Barcelay Ana P. M. Tavares Maria Goreti Ferreira Sales Isabelle Cordeiro Jaila Dias Borges Lalwani Robert Matos Henrique Fonseca Filho Spartaco Astolfi-Filho Ştefan Ţălu Pritesh Lalwani Walter Ricardo Brito An Electrochemical Immunosensor Based on Carboxylated Graphene/SPCE for IgG-SARS-CoV-2 Nucleocapsid Determination Biosensors immunosensor SARS-CoV-2 N protein |
| title | An Electrochemical Immunosensor Based on Carboxylated Graphene/SPCE for IgG-SARS-CoV-2 Nucleocapsid Determination |
| title_full | An Electrochemical Immunosensor Based on Carboxylated Graphene/SPCE for IgG-SARS-CoV-2 Nucleocapsid Determination |
| title_fullStr | An Electrochemical Immunosensor Based on Carboxylated Graphene/SPCE for IgG-SARS-CoV-2 Nucleocapsid Determination |
| title_full_unstemmed | An Electrochemical Immunosensor Based on Carboxylated Graphene/SPCE for IgG-SARS-CoV-2 Nucleocapsid Determination |
| title_short | An Electrochemical Immunosensor Based on Carboxylated Graphene/SPCE for IgG-SARS-CoV-2 Nucleocapsid Determination |
| title_sort | electrochemical immunosensor based on carboxylated graphene spce for igg sars cov 2 nucleocapsid determination |
| topic | immunosensor SARS-CoV-2 N protein |
| url | https://www.mdpi.com/2079-6374/12/12/1161 |
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