Type II transmembrane serine proteases 4 (TMPRSS4) promotes proliferation, invasion and epithelial–mesenchymal transition in endometrial carcinoma cells (HEC1A and Ishikawa) via activation of MAPK and AKT
Endometrial cancer is the most common gynecological cancer in the developed countries. Type II transmembrane serine proteases 4 (TMPRSS4) is a newly discovered transmembrane protein, which may be related to the invasion, metastasis of the tumor and the poor prognosis. This study aims to investigate...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2021-07-01
|
| Series: | Animal Cells and Systems |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/19768354.2021.1944311 |
| _version_ | 1818724287142428672 |
|---|---|
| author | Huan Xiao Zhian Zhang Dan Peng Chunqing Wei Benling Ma |
| author_facet | Huan Xiao Zhian Zhang Dan Peng Chunqing Wei Benling Ma |
| author_sort | Huan Xiao |
| collection | DOAJ |
| description | Endometrial cancer is the most common gynecological cancer in the developed countries. Type II transmembrane serine proteases 4 (TMPRSS4) is a newly discovered transmembrane protein, which may be related to the invasion, metastasis of the tumor and the poor prognosis. This study aims to investigate the role of TMPRSS4 in endometrial cancer and the detailed molecular mechanism. The results showed that TMPRSS4 was highly expressed in human endometrial cancer cells (HEC1A and Ishikawa). TMPRSS4 knockdown inhibited proliferation of endometrial cancer cells. In TMPRSS4 knockdown cells, the invasion of cells was significantly supressed. The expression of E-cadherin was significantly enhanced, while the levels of fibronectin and vimentin decreased in TMPRSS4 knockdown cells, which indicated thatTMPRSS4 knockdown attenuated the EMT of cancer cells. TMPRSS4 positively regulated the activation of MAPK and AKT signaling pathways in endometrial cancer. In conclusion, this study indicated that TMPRSS4 may be associated with the progression of endometrial cancer through promoting proliferation, invasion and EMT via activation of MAPK and AKT in endometrial cancer cells. TMPRSS4 may be a new and more effective target or therapeutic strategy for treating endometrial cancer. |
| first_indexed | 2024-12-17T21:24:01Z |
| format | Article |
| id | doaj.art-b900361588ff480a90c832db552d34f4 |
| institution | Directory Open Access Journal |
| issn | 1976-8354 2151-2485 |
| language | English |
| last_indexed | 2024-12-17T21:24:01Z |
| publishDate | 2021-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj.art-b900361588ff480a90c832db552d34f42022-12-21T21:32:04ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852021-07-0125421121810.1080/19768354.2021.19443111944311Type II transmembrane serine proteases 4 (TMPRSS4) promotes proliferation, invasion and epithelial–mesenchymal transition in endometrial carcinoma cells (HEC1A and Ishikawa) via activation of MAPK and AKTHuan Xiao0Zhian Zhang1Dan Peng2Chunqing Wei3Benling Ma4Huanggang Central Hospital Affiliated to Changjiang UniversityHuanggang Central Hospital Affiliated to Changjiang UniversityHuanggang Central Hospital Affiliated to Changjiang UniversityHuanggang Central Hospital Affiliated to Changjiang UniversityThe Second Affiliated Hospital of Hunan University of Chinese MedicineEndometrial cancer is the most common gynecological cancer in the developed countries. Type II transmembrane serine proteases 4 (TMPRSS4) is a newly discovered transmembrane protein, which may be related to the invasion, metastasis of the tumor and the poor prognosis. This study aims to investigate the role of TMPRSS4 in endometrial cancer and the detailed molecular mechanism. The results showed that TMPRSS4 was highly expressed in human endometrial cancer cells (HEC1A and Ishikawa). TMPRSS4 knockdown inhibited proliferation of endometrial cancer cells. In TMPRSS4 knockdown cells, the invasion of cells was significantly supressed. The expression of E-cadherin was significantly enhanced, while the levels of fibronectin and vimentin decreased in TMPRSS4 knockdown cells, which indicated thatTMPRSS4 knockdown attenuated the EMT of cancer cells. TMPRSS4 positively regulated the activation of MAPK and AKT signaling pathways in endometrial cancer. In conclusion, this study indicated that TMPRSS4 may be associated with the progression of endometrial cancer through promoting proliferation, invasion and EMT via activation of MAPK and AKT in endometrial cancer cells. TMPRSS4 may be a new and more effective target or therapeutic strategy for treating endometrial cancer.http://dx.doi.org/10.1080/19768354.2021.1944311type ii transmembrane serine proteases 4 (tmprss4)endometrial cancerepithelial–mesenchymal transitionmapkakt |
| spellingShingle | Huan Xiao Zhian Zhang Dan Peng Chunqing Wei Benling Ma Type II transmembrane serine proteases 4 (TMPRSS4) promotes proliferation, invasion and epithelial–mesenchymal transition in endometrial carcinoma cells (HEC1A and Ishikawa) via activation of MAPK and AKT Animal Cells and Systems type ii transmembrane serine proteases 4 (tmprss4) endometrial cancer epithelial–mesenchymal transition mapk akt |
| title | Type II transmembrane serine proteases 4 (TMPRSS4) promotes proliferation, invasion and epithelial–mesenchymal transition in endometrial carcinoma cells (HEC1A and Ishikawa) via activation of MAPK and AKT |
| title_full | Type II transmembrane serine proteases 4 (TMPRSS4) promotes proliferation, invasion and epithelial–mesenchymal transition in endometrial carcinoma cells (HEC1A and Ishikawa) via activation of MAPK and AKT |
| title_fullStr | Type II transmembrane serine proteases 4 (TMPRSS4) promotes proliferation, invasion and epithelial–mesenchymal transition in endometrial carcinoma cells (HEC1A and Ishikawa) via activation of MAPK and AKT |
| title_full_unstemmed | Type II transmembrane serine proteases 4 (TMPRSS4) promotes proliferation, invasion and epithelial–mesenchymal transition in endometrial carcinoma cells (HEC1A and Ishikawa) via activation of MAPK and AKT |
| title_short | Type II transmembrane serine proteases 4 (TMPRSS4) promotes proliferation, invasion and epithelial–mesenchymal transition in endometrial carcinoma cells (HEC1A and Ishikawa) via activation of MAPK and AKT |
| title_sort | type ii transmembrane serine proteases 4 tmprss4 promotes proliferation invasion and epithelial mesenchymal transition in endometrial carcinoma cells hec1a and ishikawa via activation of mapk and akt |
| topic | type ii transmembrane serine proteases 4 (tmprss4) endometrial cancer epithelial–mesenchymal transition mapk akt |
| url | http://dx.doi.org/10.1080/19768354.2021.1944311 |
| work_keys_str_mv | AT huanxiao typeiitransmembraneserineproteases4tmprss4promotesproliferationinvasionandepithelialmesenchymaltransitioninendometrialcarcinomacellshec1aandishikawaviaactivationofmapkandakt AT zhianzhang typeiitransmembraneserineproteases4tmprss4promotesproliferationinvasionandepithelialmesenchymaltransitioninendometrialcarcinomacellshec1aandishikawaviaactivationofmapkandakt AT danpeng typeiitransmembraneserineproteases4tmprss4promotesproliferationinvasionandepithelialmesenchymaltransitioninendometrialcarcinomacellshec1aandishikawaviaactivationofmapkandakt AT chunqingwei typeiitransmembraneserineproteases4tmprss4promotesproliferationinvasionandepithelialmesenchymaltransitioninendometrialcarcinomacellshec1aandishikawaviaactivationofmapkandakt AT benlingma typeiitransmembraneserineproteases4tmprss4promotesproliferationinvasionandepithelialmesenchymaltransitioninendometrialcarcinomacellshec1aandishikawaviaactivationofmapkandakt |