Production and development of porcine tetraploid parthenogenetic embryos
The aim of this study was to produce porcine tetraploid (4N) parthenogenetic embryos using various methods and evaluate their developmental potential. In method 1 (M1), porcine 4N parthenogenetic embryos were obtained by inhibiting extrusio...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korean Society of Animal Sciences and Technology
2019-07-01
|
| Series: | Journal of Animal Science and Technology |
| Subjects: | |
| Online Access: | http://www.ejast.org/archive/view_article?pid=jast-61-4-225 |
| Summary: | The aim of this study was to produce porcine tetraploid (4N) parthenogenetic
embryos using various methods and evaluate their developmental potential. In
method 1 (M1), porcine 4N parthenogenetic embryos were obtained by inhibiting
extrusion of both first (PB1) and second (PB2) polar bodies; in methods 2 (M2)
and 3 (M3), 4N parthenogenetic embryos were obtained by electrofusion of 2-cell
stage diploid parthenogenetic embryos derived from inhibition of PB2 or PB1
extrusion, respectively. We found no differences in the rates of cleavage or
blastocyst formation or the proportion of 4N embryos among M1, M2, and M3
groups. The different methods also did not influence apoptosis rates (number of
TUNEL-positive cells/number of total cells) or expression levels of
apoptosis-related BAX and BCL2L1 genes.
However, total cell and EdU (5-ethynyl-2’-deoxyuridine)-positive cell
numbers in 4N parthenogenetic blastocysts derived from M1 were higher
(p < 0.05) than those for M2 and M3 groups. Our
results suggest that, although porcine 4N parthenogenetic embryos could be
produced by a variety of methods, inhibition of PB1 and PB2 extrusion (M1) is
superior to electrofusion of 2-cell stage diploid parthenogenetic embryos
derived from inhibition of PB2 (M2) or PB1 (M3) extrusion. |
|---|---|
| ISSN: | 2672-0191 2055-0391 |