High-Performance Green Extraction of Polyphenolic Antioxidants from <i>Salvia fruticosa</i> Using Cyclodextrins: Optimization, Kinetics, and Composition
<i>S. fruticosa</i>, collectively known as Cretan sage, is a medicinal plant to which a number of bioactivities have been attributed. In spite of its importance in nutrition and pharmacy, reports on the extraction of major polyphenols using sustainable processes are particularly limited....
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MDPI AG
2020-05-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/10/3447 |
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| author | Spyros Grigorakis Amina Benchennouf Abedalghani Halahlah Dimitris P. Makris |
| author_facet | Spyros Grigorakis Amina Benchennouf Abedalghani Halahlah Dimitris P. Makris |
| author_sort | Spyros Grigorakis |
| collection | DOAJ |
| description | <i>S. fruticosa</i>, collectively known as Cretan sage, is a medicinal plant to which a number of bioactivities have been attributed. In spite of its importance in nutrition and pharmacy, reports on the extraction of major polyphenols using sustainable processes are particularly limited. In this study, three common cyclodextrins, namely, methyl β-cyclodextrin (m-β-CD), hydroxypropyl β-cyclodextrin (HP-β-CD), and β-cyclodextrin (β-CD), were tested as green boosters of aqueous extraction of polyphenols from aerial parts of <i>S. fruticosa</i>. To examine simultaneously important extraction parameters, including the concentration of cyclodextrins (<i>C</i><sub>CD</sub>), pH, and liquid-to-solid ratio (R<sub>L/S</sub>), a Box–Behnken design was chosen, with three central points. Temperature effects on the extraction yield were also considered, by carrying out kinetics. The results showed that m-β-CD was the most effective extraction booster, providing total polyphenols yields that amounted to 98.39 mg gallic acid equivalents g<sup>−1</sup> dry mass. The kinetic assay demonstrated that extraction was highly effective at 80 °C, increasing significantly polyphenol yield, as well as the ferric-reducing power and antiradical activity of the extracts. It was also proven that extraction with m-β-CD was the least energy-demanding process. Liquid chromatography-tandem mass spectrometry examination revealed that m-β-CD might possess higher affinity for luteolin 7-<i>O</i>-glucuronide extraction, but β-CD for rosmarinic acid extraction. |
| first_indexed | 2024-03-10T19:47:01Z |
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| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T19:47:01Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj.art-b90c3c41e3734e56b83b2499382713452023-11-20T00:43:20ZengMDPI AGApplied Sciences2076-34172020-05-011010344710.3390/app10103447High-Performance Green Extraction of Polyphenolic Antioxidants from <i>Salvia fruticosa</i> Using Cyclodextrins: Optimization, Kinetics, and CompositionSpyros Grigorakis0Amina Benchennouf1Abedalghani Halahlah2Dimitris P. Makris3Food Quality & Chemistry of Natural Products, Mediterranean Agronomic Institute of Chania (M.A.I.Ch.), International Centre for Advanced Mediterranean Agronomic Studies (CIHEAM), P.O. Box 85, Chania 73100, GreeceFood Quality & Chemistry of Natural Products, Mediterranean Agronomic Institute of Chania (M.A.I.Ch.), International Centre for Advanced Mediterranean Agronomic Studies (CIHEAM), P.O. Box 85, Chania 73100, GreeceFood Quality & Chemistry of Natural Products, Mediterranean Agronomic Institute of Chania (M.A.I.Ch.), International Centre for Advanced Mediterranean Agronomic Studies (CIHEAM), P.O. Box 85, Chania 73100, GreeceGreen Processes & Biorefinery Group, Department of Food Science & Nutrition, School of Agricultural Sciences, University of Thessaly, N. Temponera Street, Karditsa 43100, Greece<i>S. fruticosa</i>, collectively known as Cretan sage, is a medicinal plant to which a number of bioactivities have been attributed. In spite of its importance in nutrition and pharmacy, reports on the extraction of major polyphenols using sustainable processes are particularly limited. In this study, three common cyclodextrins, namely, methyl β-cyclodextrin (m-β-CD), hydroxypropyl β-cyclodextrin (HP-β-CD), and β-cyclodextrin (β-CD), were tested as green boosters of aqueous extraction of polyphenols from aerial parts of <i>S. fruticosa</i>. To examine simultaneously important extraction parameters, including the concentration of cyclodextrins (<i>C</i><sub>CD</sub>), pH, and liquid-to-solid ratio (R<sub>L/S</sub>), a Box–Behnken design was chosen, with three central points. Temperature effects on the extraction yield were also considered, by carrying out kinetics. The results showed that m-β-CD was the most effective extraction booster, providing total polyphenols yields that amounted to 98.39 mg gallic acid equivalents g<sup>−1</sup> dry mass. The kinetic assay demonstrated that extraction was highly effective at 80 °C, increasing significantly polyphenol yield, as well as the ferric-reducing power and antiradical activity of the extracts. It was also proven that extraction with m-β-CD was the least energy-demanding process. Liquid chromatography-tandem mass spectrometry examination revealed that m-β-CD might possess higher affinity for luteolin 7-<i>O</i>-glucuronide extraction, but β-CD for rosmarinic acid extraction.https://www.mdpi.com/2076-3417/10/10/3447antioxidantscyclodextrinsextraction kineticsgreen extractionpolyphenols<i>Salvia fruticosa</i> |
| spellingShingle | Spyros Grigorakis Amina Benchennouf Abedalghani Halahlah Dimitris P. Makris High-Performance Green Extraction of Polyphenolic Antioxidants from <i>Salvia fruticosa</i> Using Cyclodextrins: Optimization, Kinetics, and Composition Applied Sciences antioxidants cyclodextrins extraction kinetics green extraction polyphenols <i>Salvia fruticosa</i> |
| title | High-Performance Green Extraction of Polyphenolic Antioxidants from <i>Salvia fruticosa</i> Using Cyclodextrins: Optimization, Kinetics, and Composition |
| title_full | High-Performance Green Extraction of Polyphenolic Antioxidants from <i>Salvia fruticosa</i> Using Cyclodextrins: Optimization, Kinetics, and Composition |
| title_fullStr | High-Performance Green Extraction of Polyphenolic Antioxidants from <i>Salvia fruticosa</i> Using Cyclodextrins: Optimization, Kinetics, and Composition |
| title_full_unstemmed | High-Performance Green Extraction of Polyphenolic Antioxidants from <i>Salvia fruticosa</i> Using Cyclodextrins: Optimization, Kinetics, and Composition |
| title_short | High-Performance Green Extraction of Polyphenolic Antioxidants from <i>Salvia fruticosa</i> Using Cyclodextrins: Optimization, Kinetics, and Composition |
| title_sort | high performance green extraction of polyphenolic antioxidants from i salvia fruticosa i using cyclodextrins optimization kinetics and composition |
| topic | antioxidants cyclodextrins extraction kinetics green extraction polyphenols <i>Salvia fruticosa</i> |
| url | https://www.mdpi.com/2076-3417/10/10/3447 |
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