The Cytotoxic and Apoptosis Effects of Chloroform Extracts of Auricularia auricula on Cervical Cancer Cells
Auricularia auricula is an edible mushroom cultivated in Indonesia that has been known to have potential properties of bioactive compounds than can be used for medicinal purpose. This study aimed to examine the cytotoxic and apoptosis effect of chloroform extract of A. auricula on cervical cancer ce...
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| Format: | Article |
| Language: | English |
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Universitas Negeri Semarang
2019-04-01
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| Series: | Biosaintifika: Journal of Biology & Biology Education |
| Subjects: | |
| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/15492 |
| _version_ | 1818553318184583168 |
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| author | Arif Rahman Hikam Nuraeni Ekowati Hernayanti Hernayanti |
| author_facet | Arif Rahman Hikam Nuraeni Ekowati Hernayanti Hernayanti |
| author_sort | Arif Rahman Hikam |
| collection | DOAJ |
| description | Auricularia auricula is an edible mushroom cultivated in Indonesia that has been known to have potential properties of bioactive compounds than can be used for medicinal purpose. This study aimed to examine the cytotoxic and apoptosis effect of chloroform extract of A. auricula on cervical cancer cells in vitro. The research design was in vitro experimental research. Cytotoxic tests was using an MTT [3-(4,5-dimetiltiazol-2-yl)-2.5-diphenyl tertrazolium bromide] assay and apoptosis test was using double staining method. Test of bioactive compounds was carried out using GCMS. Cytotoxic effect were analyzed by linear regression and apoptosis test was analyzed descriptively. Chloroform extract of mycelium A. auricula showed the best results with IC50 = 264.87 µg/ml. An important finding obtained after the double staining process was that chloroform extract of A. auricula can induce HeLa cells death by apoptosis. GCMS test results showed that the extracts containing limonene and piperidinone which are the anticancer bioactive compounds. In conclusion, the chloroform extracts of A. auricula has the potential to inhibit the growth of cervical cancer cells. The benefit of this study are expected to provide information about the anticancer potential of extract of A. auricula against cervical cancer cells, thus contributing to the development of alternative anticancer treatments from natural product. |
| first_indexed | 2024-12-12T09:24:18Z |
| format | Article |
| id | doaj.art-b90c4e5ffe1e472393d7bf23256f0869 |
| institution | Directory Open Access Journal |
| issn | 2085-191X 2338-7610 |
| language | English |
| last_indexed | 2024-12-12T09:24:18Z |
| publishDate | 2019-04-01 |
| publisher | Universitas Negeri Semarang |
| record_format | Article |
| series | Biosaintifika: Journal of Biology & Biology Education |
| spelling | doaj.art-b90c4e5ffe1e472393d7bf23256f08692022-12-22T00:29:05ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102019-04-01111323810.15294/biosaintifika.v11i1.154928451The Cytotoxic and Apoptosis Effects of Chloroform Extracts of Auricularia auricula on Cervical Cancer CellsArif Rahman Hikam0Nuraeni Ekowati1Hernayanti Hernayanti2Faculty of Biology, Universitas Jenderal Soedirman, IndonesiaFaculty of Biology, Universitas Jenderal Soedirman, IndonesiaFaculty of Biology, Universitas Jenderal Soedirman, IndonesiaAuricularia auricula is an edible mushroom cultivated in Indonesia that has been known to have potential properties of bioactive compounds than can be used for medicinal purpose. This study aimed to examine the cytotoxic and apoptosis effect of chloroform extract of A. auricula on cervical cancer cells in vitro. The research design was in vitro experimental research. Cytotoxic tests was using an MTT [3-(4,5-dimetiltiazol-2-yl)-2.5-diphenyl tertrazolium bromide] assay and apoptosis test was using double staining method. Test of bioactive compounds was carried out using GCMS. Cytotoxic effect were analyzed by linear regression and apoptosis test was analyzed descriptively. Chloroform extract of mycelium A. auricula showed the best results with IC50 = 264.87 µg/ml. An important finding obtained after the double staining process was that chloroform extract of A. auricula can induce HeLa cells death by apoptosis. GCMS test results showed that the extracts containing limonene and piperidinone which are the anticancer bioactive compounds. In conclusion, the chloroform extracts of A. auricula has the potential to inhibit the growth of cervical cancer cells. The benefit of this study are expected to provide information about the anticancer potential of extract of A. auricula against cervical cancer cells, thus contributing to the development of alternative anticancer treatments from natural product.https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/15492auricularia auriculahela cells,cytotoxicapoptosis |
| spellingShingle | Arif Rahman Hikam Nuraeni Ekowati Hernayanti Hernayanti The Cytotoxic and Apoptosis Effects of Chloroform Extracts of Auricularia auricula on Cervical Cancer Cells Biosaintifika: Journal of Biology & Biology Education auricularia auricula hela cells, cytotoxic apoptosis |
| title | The Cytotoxic and Apoptosis Effects of Chloroform Extracts of Auricularia auricula on Cervical Cancer Cells |
| title_full | The Cytotoxic and Apoptosis Effects of Chloroform Extracts of Auricularia auricula on Cervical Cancer Cells |
| title_fullStr | The Cytotoxic and Apoptosis Effects of Chloroform Extracts of Auricularia auricula on Cervical Cancer Cells |
| title_full_unstemmed | The Cytotoxic and Apoptosis Effects of Chloroform Extracts of Auricularia auricula on Cervical Cancer Cells |
| title_short | The Cytotoxic and Apoptosis Effects of Chloroform Extracts of Auricularia auricula on Cervical Cancer Cells |
| title_sort | cytotoxic and apoptosis effects of chloroform extracts of auricularia auricula on cervical cancer cells |
| topic | auricularia auricula hela cells, cytotoxic apoptosis |
| url | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/15492 |
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