Effects of Dietary Alpha-Lipoic Acid on Growth Performance, Serum Biochemical Indexes, Liver Antioxidant Capacity and Transcriptome of Juvenile Hybrid Grouper (<i>Epinephelus fuscoguttatus</i>♀ × <i>Epinephelus polyphekadion</i>♂)
We aimed to investigate the effects of dietary alpha-lipoic acid (α-LA) on the growth performance, serum biochemical indexes, liver morphology, antioxidant capacity, and transcriptome of juvenile hybrid groupers (<i>Epinephelus fuscoguttatus</i>♀ × <i>Epinephelus polyphekadion</...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/5/887 |
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| author | Guanghai Ou Ruitao Xie Jiansheng Huang Jianpeng Huang Zhenwei Wen Yu Li Xintao Jiang Qian Ma Gang Chen |
| author_facet | Guanghai Ou Ruitao Xie Jiansheng Huang Jianpeng Huang Zhenwei Wen Yu Li Xintao Jiang Qian Ma Gang Chen |
| author_sort | Guanghai Ou |
| collection | DOAJ |
| description | We aimed to investigate the effects of dietary alpha-lipoic acid (α-LA) on the growth performance, serum biochemical indexes, liver morphology, antioxidant capacity, and transcriptome of juvenile hybrid groupers (<i>Epinephelus fuscoguttatus</i>♀ × <i>Epinephelus polyphekadion</i>♂). Four experimental diets supplemented with 0 (SL0), 0.4 (L1), 0.6 (L2), and 1.2 (L3) g/kg α-LA were formulated and fed to three replicates of juvenile hybrid grouper (24.06 ± 0.15 g) for 56 d. The results indicated that dietary 0.4 and 0.6 g/kg α-LA significantly decreased the weight gain rate in juvenile hybrid groupers. Compared with SL0, the content of total protein in the serum of L1, L2, and L3 increased significantly, and alanine aminotransferase decreased significantly. The content of albumin in the serum of L3 increased significantly, and triglyceride, total cholesterol, and aspartate aminotransferase decreased significantly. In addition, the hepatocyte morphology in L1, L2, and L3 all showed varying degrees of improvement, and the activities of glutathione peroxidase and superoxide dismutase in the liver of L2 and L3 were significantly increased. A total of 42 differentially expressed genes were screened in the transcriptome data. KEGG showed that a total of 12 pathways were significantly enriched, including the pathway related to immune function and glucose homeostasis. The expression of genes (<i>ifnk</i>, <i>prl4a1</i>, <i>prl3b1</i>, and <i>ctsl</i>) related to immune were significantly up-regulated, and the expressions of <i>gapdh</i> and <i>eno1</i> genes related to glucose homeostasis were significantly down-regulated and up-regulated, respectively. In summary, dietary supplementation of 0.4 and 0.6 g/kg α-LA inhibited the growth performance of juvenile hybrid groupers. A total of 1.2 g/kg α-LA could reduce the blood lipid level, improve hepatocyte damage, and increase the hepatic antioxidant enzyme activity. Dietary α-LA significantly affected the pathway related to immune function and glucose homeostasis. |
| first_indexed | 2024-03-11T07:32:09Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T07:32:09Z |
| publishDate | 2023-02-01 |
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| spelling | doaj.art-b90f1242676a4251802b482a76b6766b2023-11-17T07:13:42ZengMDPI AGAnimals2076-26152023-02-0113588710.3390/ani13050887Effects of Dietary Alpha-Lipoic Acid on Growth Performance, Serum Biochemical Indexes, Liver Antioxidant Capacity and Transcriptome of Juvenile Hybrid Grouper (<i>Epinephelus fuscoguttatus</i>♀ × <i>Epinephelus polyphekadion</i>♂)Guanghai Ou0Ruitao Xie1Jiansheng Huang2Jianpeng Huang3Zhenwei Wen4Yu Li5Xintao Jiang6Qian Ma7Gang Chen8Fishery College, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Evergreen Feed Industry Co., Ltd., Zhanjiang 524000, ChinaFishery College, Guangdong Ocean University, Zhanjiang 524088, ChinaFishery College, Guangdong Ocean University, Zhanjiang 524088, ChinaFishery College, Guangdong Ocean University, Zhanjiang 524088, ChinaFishery College, Guangdong Ocean University, Zhanjiang 524088, ChinaFishery College, Guangdong Ocean University, Zhanjiang 524088, ChinaFishery College, Guangdong Ocean University, Zhanjiang 524088, ChinaFishery College, Guangdong Ocean University, Zhanjiang 524088, ChinaWe aimed to investigate the effects of dietary alpha-lipoic acid (α-LA) on the growth performance, serum biochemical indexes, liver morphology, antioxidant capacity, and transcriptome of juvenile hybrid groupers (<i>Epinephelus fuscoguttatus</i>♀ × <i>Epinephelus polyphekadion</i>♂). Four experimental diets supplemented with 0 (SL0), 0.4 (L1), 0.6 (L2), and 1.2 (L3) g/kg α-LA were formulated and fed to three replicates of juvenile hybrid grouper (24.06 ± 0.15 g) for 56 d. The results indicated that dietary 0.4 and 0.6 g/kg α-LA significantly decreased the weight gain rate in juvenile hybrid groupers. Compared with SL0, the content of total protein in the serum of L1, L2, and L3 increased significantly, and alanine aminotransferase decreased significantly. The content of albumin in the serum of L3 increased significantly, and triglyceride, total cholesterol, and aspartate aminotransferase decreased significantly. In addition, the hepatocyte morphology in L1, L2, and L3 all showed varying degrees of improvement, and the activities of glutathione peroxidase and superoxide dismutase in the liver of L2 and L3 were significantly increased. A total of 42 differentially expressed genes were screened in the transcriptome data. KEGG showed that a total of 12 pathways were significantly enriched, including the pathway related to immune function and glucose homeostasis. The expression of genes (<i>ifnk</i>, <i>prl4a1</i>, <i>prl3b1</i>, and <i>ctsl</i>) related to immune were significantly up-regulated, and the expressions of <i>gapdh</i> and <i>eno1</i> genes related to glucose homeostasis were significantly down-regulated and up-regulated, respectively. In summary, dietary supplementation of 0.4 and 0.6 g/kg α-LA inhibited the growth performance of juvenile hybrid groupers. A total of 1.2 g/kg α-LA could reduce the blood lipid level, improve hepatocyte damage, and increase the hepatic antioxidant enzyme activity. Dietary α-LA significantly affected the pathway related to immune function and glucose homeostasis.https://www.mdpi.com/2076-2615/13/5/887juvenile hybrid grouperα-lipoic acidgrowth performanceserum biochemical indexesantioxidant capacitytranscriptome |
| spellingShingle | Guanghai Ou Ruitao Xie Jiansheng Huang Jianpeng Huang Zhenwei Wen Yu Li Xintao Jiang Qian Ma Gang Chen Effects of Dietary Alpha-Lipoic Acid on Growth Performance, Serum Biochemical Indexes, Liver Antioxidant Capacity and Transcriptome of Juvenile Hybrid Grouper (<i>Epinephelus fuscoguttatus</i>♀ × <i>Epinephelus polyphekadion</i>♂) Animals juvenile hybrid grouper α-lipoic acid growth performance serum biochemical indexes antioxidant capacity transcriptome |
| title | Effects of Dietary Alpha-Lipoic Acid on Growth Performance, Serum Biochemical Indexes, Liver Antioxidant Capacity and Transcriptome of Juvenile Hybrid Grouper (<i>Epinephelus fuscoguttatus</i>♀ × <i>Epinephelus polyphekadion</i>♂) |
| title_full | Effects of Dietary Alpha-Lipoic Acid on Growth Performance, Serum Biochemical Indexes, Liver Antioxidant Capacity and Transcriptome of Juvenile Hybrid Grouper (<i>Epinephelus fuscoguttatus</i>♀ × <i>Epinephelus polyphekadion</i>♂) |
| title_fullStr | Effects of Dietary Alpha-Lipoic Acid on Growth Performance, Serum Biochemical Indexes, Liver Antioxidant Capacity and Transcriptome of Juvenile Hybrid Grouper (<i>Epinephelus fuscoguttatus</i>♀ × <i>Epinephelus polyphekadion</i>♂) |
| title_full_unstemmed | Effects of Dietary Alpha-Lipoic Acid on Growth Performance, Serum Biochemical Indexes, Liver Antioxidant Capacity and Transcriptome of Juvenile Hybrid Grouper (<i>Epinephelus fuscoguttatus</i>♀ × <i>Epinephelus polyphekadion</i>♂) |
| title_short | Effects of Dietary Alpha-Lipoic Acid on Growth Performance, Serum Biochemical Indexes, Liver Antioxidant Capacity and Transcriptome of Juvenile Hybrid Grouper (<i>Epinephelus fuscoguttatus</i>♀ × <i>Epinephelus polyphekadion</i>♂) |
| title_sort | effects of dietary alpha lipoic acid on growth performance serum biochemical indexes liver antioxidant capacity and transcriptome of juvenile hybrid grouper i epinephelus fuscoguttatus i ♀ i epinephelus polyphekadion i ♂ |
| topic | juvenile hybrid grouper α-lipoic acid growth performance serum biochemical indexes antioxidant capacity transcriptome |
| url | https://www.mdpi.com/2076-2615/13/5/887 |
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