Factors Influencing the Yield of Progenitor Cells in Bone Marrow Aspiration Concentrate—A Retrospective Analysis of 58 Patients
This study aims to identify the role of subjective factors (age, sex, and comorbidities) and procedure-specific factors (aspiration volume) in influencing the yield of progenitor cells in bone marrow aspiration concentrate (BMAC) harvested from the iliac crest. A retrospective analysis was conducted...
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MDPI AG
2023-03-01
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| author | Sathish Muthu Madhan Jeyaraman Aditya Narula V. R. Ravi Avinash Gandi Manish Khanna Nicola Maffulli Ashim Gupta |
| author_facet | Sathish Muthu Madhan Jeyaraman Aditya Narula V. R. Ravi Avinash Gandi Manish Khanna Nicola Maffulli Ashim Gupta |
| author_sort | Sathish Muthu |
| collection | DOAJ |
| description | This study aims to identify the role of subjective factors (age, sex, and comorbidities) and procedure-specific factors (aspiration volume) in influencing the yield of progenitor cells in bone marrow aspiration concentrate (BMAC) harvested from the iliac crest. A retrospective analysis was conducted on 58 patients (male:female = 31:27; mean age: 52.56 ± 18.14 years) who underwent BMAC therapy between January 2020 and June 2021. The factors analyzed include individual factors such as age, sex, and comorbid conditions, and procedural factors such as aspirate volume. The mononuclear cell (MNC) count and colony-forming unit (CFU) assay were used to assess the yield of progenitors in the aspirate. Pearson’s correlation test was performed for the age, aspirate volume, and outcome parameters, such as MNC and CFU. We used the chi-square test to analyze the role of sex and comorbidities on cellular yield. The mean volume of aspirate used for BMAC therapy was 66.65 (±17.82) mL. The mean MNC count of the BMAC was 19.94 (±16.34) × 10<sup>6</sup> cells, which formed 11 (±12) CFUs. Evidence of statistically significant positive associations was noted between the CFUs developed from the BMAC and the MNC count within them (r = 0.95, <i>p</i> < 0.001). The sex of the individual did not play any significant role in MNC count (<i>p</i> = 0.092) or CFUs formed (<i>p</i> = 0.448). The age of the individual showed evidence of a statistically significant negative association with the MNC count (r = −0.681, <i>p</i> < 0.001) and CFUs (r = −0.693, <i>p</i> < 0.001), as did the aspiration volume with the MNC count (r = −0.740, <i>p</i> < 0.001) and CFUs (r = −0.629, <i>p</i> < 0.001). We also noted a significant reduction in the MNC count (<i>p</i> = 0.002) and CFUs formed (<i>p</i> = 0.004) when the patients presented comorbidities. Individual factors such as age, comorbid conditions, and procedure factors such as aspirate volume significantly affected the yield of progenitor cells in the BMAC. The sex of the individual did not influence the yield of progenitor cells in BMAC. |
| first_indexed | 2024-03-11T06:54:50Z |
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| spelling | doaj.art-b90f36a1a3d2499097800658debb00202023-11-17T09:44:55ZengMDPI AGBiomedicines2227-90592023-03-0111373810.3390/biomedicines11030738Factors Influencing the Yield of Progenitor Cells in Bone Marrow Aspiration Concentrate—A Retrospective Analysis of 58 PatientsSathish Muthu0Madhan Jeyaraman1Aditya Narula2V. R. Ravi3Avinash Gandi4Manish Khanna5Nicola Maffulli6Ashim Gupta7Indian Stem Cell Study Group (ISCSG) Association, Lucknow 226010, Uttar Pradesh, IndiaIndian Stem Cell Study Group (ISCSG) Association, Lucknow 226010, Uttar Pradesh, IndiaDepartment of Orthopaedics, Aakaar Bone Care, Kanpur 208002, Uttar Pradesh, IndiaIndian Stem Cell Study Group (ISCSG) Association, Lucknow 226010, Uttar Pradesh, IndiaIndian Stem Cell Study Group (ISCSG) Association, Lucknow 226010, Uttar Pradesh, IndiaIndian Stem Cell Study Group (ISCSG) Association, Lucknow 226010, Uttar Pradesh, IndiaDepartment of Musculoskeletal Disorders, School of Medicine and Surgery, University of Salerno, 84084 Fisciano, ItalyIndian Stem Cell Study Group (ISCSG) Association, Lucknow 226010, Uttar Pradesh, IndiaThis study aims to identify the role of subjective factors (age, sex, and comorbidities) and procedure-specific factors (aspiration volume) in influencing the yield of progenitor cells in bone marrow aspiration concentrate (BMAC) harvested from the iliac crest. A retrospective analysis was conducted on 58 patients (male:female = 31:27; mean age: 52.56 ± 18.14 years) who underwent BMAC therapy between January 2020 and June 2021. The factors analyzed include individual factors such as age, sex, and comorbid conditions, and procedural factors such as aspirate volume. The mononuclear cell (MNC) count and colony-forming unit (CFU) assay were used to assess the yield of progenitors in the aspirate. Pearson’s correlation test was performed for the age, aspirate volume, and outcome parameters, such as MNC and CFU. We used the chi-square test to analyze the role of sex and comorbidities on cellular yield. The mean volume of aspirate used for BMAC therapy was 66.65 (±17.82) mL. The mean MNC count of the BMAC was 19.94 (±16.34) × 10<sup>6</sup> cells, which formed 11 (±12) CFUs. Evidence of statistically significant positive associations was noted between the CFUs developed from the BMAC and the MNC count within them (r = 0.95, <i>p</i> < 0.001). The sex of the individual did not play any significant role in MNC count (<i>p</i> = 0.092) or CFUs formed (<i>p</i> = 0.448). The age of the individual showed evidence of a statistically significant negative association with the MNC count (r = −0.681, <i>p</i> < 0.001) and CFUs (r = −0.693, <i>p</i> < 0.001), as did the aspiration volume with the MNC count (r = −0.740, <i>p</i> < 0.001) and CFUs (r = −0.629, <i>p</i> < 0.001). We also noted a significant reduction in the MNC count (<i>p</i> = 0.002) and CFUs formed (<i>p</i> = 0.004) when the patients presented comorbidities. Individual factors such as age, comorbid conditions, and procedure factors such as aspirate volume significantly affected the yield of progenitor cells in the BMAC. The sex of the individual did not influence the yield of progenitor cells in BMAC.https://www.mdpi.com/2227-9059/11/3/738BMACbone marrow aspiration concentratecellular yieldprogenitor cellsregenerative medicinemononuclear cell |
| spellingShingle | Sathish Muthu Madhan Jeyaraman Aditya Narula V. R. Ravi Avinash Gandi Manish Khanna Nicola Maffulli Ashim Gupta Factors Influencing the Yield of Progenitor Cells in Bone Marrow Aspiration Concentrate—A Retrospective Analysis of 58 Patients Biomedicines BMAC bone marrow aspiration concentrate cellular yield progenitor cells regenerative medicine mononuclear cell |
| title | Factors Influencing the Yield of Progenitor Cells in Bone Marrow Aspiration Concentrate—A Retrospective Analysis of 58 Patients |
| title_full | Factors Influencing the Yield of Progenitor Cells in Bone Marrow Aspiration Concentrate—A Retrospective Analysis of 58 Patients |
| title_fullStr | Factors Influencing the Yield of Progenitor Cells in Bone Marrow Aspiration Concentrate—A Retrospective Analysis of 58 Patients |
| title_full_unstemmed | Factors Influencing the Yield of Progenitor Cells in Bone Marrow Aspiration Concentrate—A Retrospective Analysis of 58 Patients |
| title_short | Factors Influencing the Yield of Progenitor Cells in Bone Marrow Aspiration Concentrate—A Retrospective Analysis of 58 Patients |
| title_sort | factors influencing the yield of progenitor cells in bone marrow aspiration concentrate a retrospective analysis of 58 patients |
| topic | BMAC bone marrow aspiration concentrate cellular yield progenitor cells regenerative medicine mononuclear cell |
| url | https://www.mdpi.com/2227-9059/11/3/738 |
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