Role of Physico-Chemical and Cellular Conditions on the Bone Repair Potential of Plastically Compressed Collagen Hydrogels
Since their first description nearly 20 years ago, dense collagen hydrogels obtained by plastic compression have become popular scaffolds in tissue engineering. In particular, when seeded with dental pulp stem cells, they have demonstrated a great in vivo potential in cranial bone repair. Here, we i...
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| Format: | Article |
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MDPI AG
2024-02-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/10/2/130 |
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| author | Daline Mbitta Akoa Ludovic Sicard Christophe Hélary Coralie Torrens Brigitte Baroukh Anne Poliard Thibaud Coradin |
| author_facet | Daline Mbitta Akoa Ludovic Sicard Christophe Hélary Coralie Torrens Brigitte Baroukh Anne Poliard Thibaud Coradin |
| author_sort | Daline Mbitta Akoa |
| collection | DOAJ |
| description | Since their first description nearly 20 years ago, dense collagen hydrogels obtained by plastic compression have become popular scaffolds in tissue engineering. In particular, when seeded with dental pulp stem cells, they have demonstrated a great in vivo potential in cranial bone repair. Here, we investigated how physico-chemical and cell-seeding conditions could influence the formation and in vitro mineralization of these cellularized scaffolds. A qualitative assessment demonstrated that the gel stability before and after compression was highly sensitive to the conditions of fibrillogenesis, especially initial acid acetic and buffer concentrations. Gels with similar rheological properties but different fibrillar structures that exhibited different stabilities when used for the 3D culture of Stem cells from Human Exfoliated Deciduous teeth (SHEDs) could be prepared. Finally, in our optimal physico-chemical conditions, mineralization could be achieved only using human dental pulp stem cells (hDPSCs) at a high cell density. These results highlight the key role of fibrillogenic conditions and cell type/density on the bone repair potential of cell-laden plastically compressed collagen hydrogels. |
| first_indexed | 2024-03-07T22:31:57Z |
| format | Article |
| id | doaj.art-b9111396777640e7ad562a8095f254e0 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-07T22:31:57Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-b9111396777640e7ad562a8095f254e02024-02-23T15:17:33ZengMDPI AGGels2310-28612024-02-0110213010.3390/gels10020130Role of Physico-Chemical and Cellular Conditions on the Bone Repair Potential of Plastically Compressed Collagen HydrogelsDaline Mbitta Akoa0Ludovic Sicard1Christophe Hélary2Coralie Torrens3Brigitte Baroukh4Anne Poliard5Thibaud Coradin6Sorbonne Université, CNRS, Laboratoire de Chimie de la Matière Condensée de Paris, 75005 Paris, FranceUniversité de Paris, UR2496 Pathologies, Imagerie et Biothérapies Orofaciales, FHU-DDS-Net, Dental School, 92120 Montrouge, FranceSorbonne Université, CNRS, Laboratoire de Chimie de la Matière Condensée de Paris, 75005 Paris, FranceUniversité de Paris, UR2496 Pathologies, Imagerie et Biothérapies Orofaciales, FHU-DDS-Net, Dental School, 92120 Montrouge, FranceUniversité de Paris, UR2496 Pathologies, Imagerie et Biothérapies Orofaciales, FHU-DDS-Net, Dental School, 92120 Montrouge, FranceUniversité de Paris, UR2496 Pathologies, Imagerie et Biothérapies Orofaciales, FHU-DDS-Net, Dental School, 92120 Montrouge, FranceSorbonne Université, CNRS, Laboratoire de Chimie de la Matière Condensée de Paris, 75005 Paris, FranceSince their first description nearly 20 years ago, dense collagen hydrogels obtained by plastic compression have become popular scaffolds in tissue engineering. In particular, when seeded with dental pulp stem cells, they have demonstrated a great in vivo potential in cranial bone repair. Here, we investigated how physico-chemical and cell-seeding conditions could influence the formation and in vitro mineralization of these cellularized scaffolds. A qualitative assessment demonstrated that the gel stability before and after compression was highly sensitive to the conditions of fibrillogenesis, especially initial acid acetic and buffer concentrations. Gels with similar rheological properties but different fibrillar structures that exhibited different stabilities when used for the 3D culture of Stem cells from Human Exfoliated Deciduous teeth (SHEDs) could be prepared. Finally, in our optimal physico-chemical conditions, mineralization could be achieved only using human dental pulp stem cells (hDPSCs) at a high cell density. These results highlight the key role of fibrillogenic conditions and cell type/density on the bone repair potential of cell-laden plastically compressed collagen hydrogels.https://www.mdpi.com/2310-2861/10/2/130hydrogelscollagendental pulp stem cellsplastic compressionbone repair |
| spellingShingle | Daline Mbitta Akoa Ludovic Sicard Christophe Hélary Coralie Torrens Brigitte Baroukh Anne Poliard Thibaud Coradin Role of Physico-Chemical and Cellular Conditions on the Bone Repair Potential of Plastically Compressed Collagen Hydrogels Gels hydrogels collagen dental pulp stem cells plastic compression bone repair |
| title | Role of Physico-Chemical and Cellular Conditions on the Bone Repair Potential of Plastically Compressed Collagen Hydrogels |
| title_full | Role of Physico-Chemical and Cellular Conditions on the Bone Repair Potential of Plastically Compressed Collagen Hydrogels |
| title_fullStr | Role of Physico-Chemical and Cellular Conditions on the Bone Repair Potential of Plastically Compressed Collagen Hydrogels |
| title_full_unstemmed | Role of Physico-Chemical and Cellular Conditions on the Bone Repair Potential of Plastically Compressed Collagen Hydrogels |
| title_short | Role of Physico-Chemical and Cellular Conditions on the Bone Repair Potential of Plastically Compressed Collagen Hydrogels |
| title_sort | role of physico chemical and cellular conditions on the bone repair potential of plastically compressed collagen hydrogels |
| topic | hydrogels collagen dental pulp stem cells plastic compression bone repair |
| url | https://www.mdpi.com/2310-2861/10/2/130 |
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