ISOLATION AND IDENTIFICATION OF MICROBIAL FLORA IN EYE AND STUDY THE EFFECT OF EYE LINER AND CONTACT LENSES USING ON THE MICROBIAL FLORA ON IT
Our studying includes culturing of the conjunctival swabs on selective media and enrichment media (Nutrient agar, Machonky agar and blood agar) from for students of Anbar university. They are (19 – 26) years old, during the period from 2008 to Feb. 2009. The number of samples are 30 swabs divided ta...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
University of Anbar
2012-06-01
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Series: | مجلة جامعة الانبار للعلوم الصرفة |
Online Access: | https://juaps.uoanbar.edu.iq/article_15437_eada0495087916cf8f193be69434064b.pdf |
Summary: | Our studying includes culturing of the conjunctival swabs on selective media and enrichment media (Nutrient agar, Machonky agar and blood agar) from for students of Anbar university. They are (19 – 26) years old, during the period from 2008 to Feb. 2009. The number of samples are 30 swabs divided taking 10 from female (using contact lens). 10 from female (using the eye liner) and (5 male, 5 female) they were a save as control into three groups (They had never worm contact lenses and eye liner). They are very deferent in microbial flora between the wearing contact lenses and who had never. The microbial flora isolated control group was Staphylococcus epidermidis, S. capitis, S. Xylosus, S. areus. They were 83.32% and Candida in 16.68%. But the microbial flora (Contamination) in contact lenses using group was the gram negative bacteria Pseudomonas aeruginosain 16.62%. Aeromonas, Klebsiella, E.coli in 8.33 for each and gram posative bacteria (S. aureus, S. epidermidis) in 25% and B. heamolytic, Streptococcus in 8.33 and it is including fungi (Aspirigilus spp., Alternaria and Candida in 8.33 for each. In the eye liner using group the 60% were negative for laboratory culturing and 40% were positive for laboratory culturing. They were Staphylococcus Epidermidis and S. aureus. The second line of study include culture for sensitivity to same intibiotic for bacteria. They are appeared that is the antibiotic Erethromycin is more effect on gram positive and gram negative bacteria they are (69.5% sensitive) then Tetracycline in 47.8% and Gentumycin in 52% but the more antibiotic which was the bacteria Resisting was Penicillin G (just 4.7% sensitive) then Ampicillin in 26.9 and Cefalexin in 35.7% because the fals using of those drugs from the people. |
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ISSN: | 1991-8941 2706-6703 |