Low expression of NCALD is associated with chemotherapy resistance and poor prognosis in epithelial ovarian cancer
Abstract Background Low expression of NCALD(neurocalcin delta) in peripheral blood of ovarian cancer patients predicts poor prognosis. However, the molecular mechanism of NCALD in ovarian cancer and its relationship with chemotherapy outcomes is unclear. The aim of this study was to investigate the...
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| Format: | Article |
| Language: | English |
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BMC
2020-03-01
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| Series: | Journal of Ovarian Research |
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| Online Access: | http://link.springer.com/article/10.1186/s13048-020-00635-6 |
| _version_ | 1797964698596933632 |
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| author | Li-yuan Feng Li Li |
| author_facet | Li-yuan Feng Li Li |
| author_sort | Li-yuan Feng |
| collection | DOAJ |
| description | Abstract Background Low expression of NCALD(neurocalcin delta) in peripheral blood of ovarian cancer patients predicts poor prognosis. However, the molecular mechanism of NCALD in ovarian cancer and its relationship with chemotherapy outcomes is unclear. The aim of this study was to investigate the potential signaling pathways of NCALD and to evaluate its ability to predict chemotherapy outcomes and prognosis. Methods High-throughput RNA sequencing data were downloaded from TCGA. GSEA explored the potential signaling pathways of NCALD. The expression of NCALD in chemotherapy sensitive and chemotherapy resistant ovarian cancer patients was detected by TCGA data and clinical samples. ROC analysis confirmed the ability of NCALD to predict chemotherapy outcomes. The association between NCALD expression and prognosis in ovarian cancer patients was assessed using Kaplan-Meier plotter. Results In patients with NCALD overexpression, genes expression related to ERK1 / 2 signaling pathway, NF-kappaB signaling pathway, TGF-β signaling pathway and immune response pathway was increased, especially ERK1 / 2 signaling pathway. The expression of NCALD in chemoresistant patients was significantly lower than chemosensitive patients. In TCGA data and immunohistochemical samples, the AUC of NCALD expression predicting chemotherapy outcome was 0.59 and 0.64, respectively. In clinical samples, low expression of NCALD was associated with poor OS and PFS. Conclusions NCALD may activate the ERK1 / 2 signaling pathway in ovarian cancer. As a new biomarker of chemotherapy sensitivity, NCALD was significantly down-regulated in chemotherapy resistance ovarian cancer patients. Low expression of NCALD in ovarian cancer is associated with poor OS and PFS. In the future, further research will be needed on the potential mechanism and clinical application value of NCALD in ovarian cancer. |
| first_indexed | 2024-04-11T01:49:07Z |
| format | Article |
| id | doaj.art-b912146b6c7a46eea8fac74ed1fd829a |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-04-11T01:49:07Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-b912146b6c7a46eea8fac74ed1fd829a2023-01-03T07:22:54ZengBMCJournal of Ovarian Research1757-22152020-03-0113111010.1186/s13048-020-00635-6Low expression of NCALD is associated with chemotherapy resistance and poor prognosis in epithelial ovarian cancerLi-yuan Feng0Li Li1Department of Gynecologic oncology, Guangxi Medical University Cancer HospitalDepartment of Gynecologic oncology, Guangxi Medical University Cancer HospitalAbstract Background Low expression of NCALD(neurocalcin delta) in peripheral blood of ovarian cancer patients predicts poor prognosis. However, the molecular mechanism of NCALD in ovarian cancer and its relationship with chemotherapy outcomes is unclear. The aim of this study was to investigate the potential signaling pathways of NCALD and to evaluate its ability to predict chemotherapy outcomes and prognosis. Methods High-throughput RNA sequencing data were downloaded from TCGA. GSEA explored the potential signaling pathways of NCALD. The expression of NCALD in chemotherapy sensitive and chemotherapy resistant ovarian cancer patients was detected by TCGA data and clinical samples. ROC analysis confirmed the ability of NCALD to predict chemotherapy outcomes. The association between NCALD expression and prognosis in ovarian cancer patients was assessed using Kaplan-Meier plotter. Results In patients with NCALD overexpression, genes expression related to ERK1 / 2 signaling pathway, NF-kappaB signaling pathway, TGF-β signaling pathway and immune response pathway was increased, especially ERK1 / 2 signaling pathway. The expression of NCALD in chemoresistant patients was significantly lower than chemosensitive patients. In TCGA data and immunohistochemical samples, the AUC of NCALD expression predicting chemotherapy outcome was 0.59 and 0.64, respectively. In clinical samples, low expression of NCALD was associated with poor OS and PFS. Conclusions NCALD may activate the ERK1 / 2 signaling pathway in ovarian cancer. As a new biomarker of chemotherapy sensitivity, NCALD was significantly down-regulated in chemotherapy resistance ovarian cancer patients. Low expression of NCALD in ovarian cancer is associated with poor OS and PFS. In the future, further research will be needed on the potential mechanism and clinical application value of NCALD in ovarian cancer.http://link.springer.com/article/10.1186/s13048-020-00635-6Ovarian cancerChemoresistanceNCALDPrognosis |
| spellingShingle | Li-yuan Feng Li Li Low expression of NCALD is associated with chemotherapy resistance and poor prognosis in epithelial ovarian cancer Journal of Ovarian Research Ovarian cancer Chemoresistance NCALD Prognosis |
| title | Low expression of NCALD is associated with chemotherapy resistance and poor prognosis in epithelial ovarian cancer |
| title_full | Low expression of NCALD is associated with chemotherapy resistance and poor prognosis in epithelial ovarian cancer |
| title_fullStr | Low expression of NCALD is associated with chemotherapy resistance and poor prognosis in epithelial ovarian cancer |
| title_full_unstemmed | Low expression of NCALD is associated with chemotherapy resistance and poor prognosis in epithelial ovarian cancer |
| title_short | Low expression of NCALD is associated with chemotherapy resistance and poor prognosis in epithelial ovarian cancer |
| title_sort | low expression of ncald is associated with chemotherapy resistance and poor prognosis in epithelial ovarian cancer |
| topic | Ovarian cancer Chemoresistance NCALD Prognosis |
| url | http://link.springer.com/article/10.1186/s13048-020-00635-6 |
| work_keys_str_mv | AT liyuanfeng lowexpressionofncaldisassociatedwithchemotherapyresistanceandpoorprognosisinepithelialovariancancer AT lili lowexpressionofncaldisassociatedwithchemotherapyresistanceandpoorprognosisinepithelialovariancancer |