Evidence that the E2-isoprostane, 15-E2t-isoprostane (8-iso-prostaglandin E2) is formed in vivo

Evidence that the E2-isoprostane, 15-E2t-isoprostane (8-iso-prostaglandin E2) is formed in vivo

D2/E2-isoprostanes are prostaglandin D2/E2-like compounds that are produced in vivo as non-enzymatic products of free radical catalyzed peroxidation of arachidonic acid. One E2-isoprostane that should be formed is 15-E2t-isoprostane (8-iso-prostaglandin E2). 15-E2t-isoprostane has been shown to exer...

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Bibliographic Details
Main Authors: Jason D. Morrow, Jennifer Scruggs, Yan Chen, William E. Zackert, L. Jackson Roberts, II
Format: Article
Language:English
Published: Elsevier 1998-08-01
Series:Journal of Lipid Research
Subjects:
lipid peroxidation
isoeicosanoids
free radicals
eicosanoid
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520321878
Description
Summary:D2/E2-isoprostanes are prostaglandin D2/E2-like compounds that are produced in vivo as non-enzymatic products of free radical catalyzed peroxidation of arachidonic acid. One E2-isoprostane that should be formed is 15-E2t-isoprostane (8-iso-prostaglandin E2). 15-E2t-isoprostane has been shown to exert potent biological activity but proof that it is formed in vivo is lacking. Evidence is now presented that 15-E2t-isoprostane is formed in vivo by demonstrating that an endogenous E2-isoprostane with a retention time on capillary GC identical with that of 15-E2t-isoprostane co-chromatographs through four high resolving HPLC purification procedures with authentic radiolabeled 15-E2t-isoprostane.—Morrow, J. D., J. Scruggs, Y. Chan, W. E. Zackert, and L. J. Roberts II. Evidence that the E2-isoprostane, 15-E2t-isoprostane (8-iso-prostaglandin E2) is formed in vivo.
ISSN:0022-2275

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