HTS-Based Monitoring of the Efficiency of Somatic Embryogenesis and Meristem Cultures Used for Virus Elimination in Grapevine
Meristem culture and somatic embryogenesis are effective tools for virus elimination of vegetatively propagated crops including grapevine (<i>Vitis vinifera</i> L.). While both have been shown to be useful to eliminate the main grapevine viruses, their efficiency differs depending on the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/12/1782 |
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| author | Mihaly Turcsan Emese Demian Tunde Varga Nikoletta Jaksa-Czotter Erno Szegedi Robert Olah Eva Varallyay |
| author_facet | Mihaly Turcsan Emese Demian Tunde Varga Nikoletta Jaksa-Czotter Erno Szegedi Robert Olah Eva Varallyay |
| author_sort | Mihaly Turcsan |
| collection | DOAJ |
| description | Meristem culture and somatic embryogenesis are effective tools for virus elimination of vegetatively propagated crops including grapevine (<i>Vitis vinifera</i> L.). While both have been shown to be useful to eliminate the main grapevine viruses, their efficiency differs depending on the virus and grapevine variety. In our work, we investigated the efficiency of these two virus elimination methods using small RNA high-throughput sequencing (HTS) and RT-PCR as virus diagnostics. Field grown mother plants of four clones representing three cultivars, infected with different viruses and viroids, were selected for elimination via somatic embryogenesis (SE) and meristem culture (ME). Our results show for the first time that using SE, elimination in mother plants was effective for all viruses, i.e., grapevine rupestris vein feathering virus (GRVFV), grapevine Syrah virus 1 (GSyV-1), Grapevine virus T (GVT) and grapevine Pinot gris virus (GPGV). This study also confirms previous studies showing that SE is a possible strategy for the elimination of GFkV, GRSPaV, HSVd, and GYSVd-1. Our results demonstrate that the efficacy of virus elimination via SE is relatively high while the purging of viroids is lower. Our work provides evidence that the efficiency of SE is comparable to that of the technically difficult ME technique, and that SE will offer a more effective strategy for the production of virus-free grapevine in the future. |
| first_indexed | 2024-03-10T14:02:19Z |
| format | Article |
| id | doaj.art-b913aaa29fce4d608fd7d327b6790de8 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T14:02:19Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-b913aaa29fce4d608fd7d327b6790de82023-11-21T01:01:10ZengMDPI AGPlants2223-77472020-12-01912178210.3390/plants9121782HTS-Based Monitoring of the Efficiency of Somatic Embryogenesis and Meristem Cultures Used for Virus Elimination in GrapevineMihaly Turcsan0Emese Demian1Tunde Varga2Nikoletta Jaksa-Czotter3Erno Szegedi4Robert Olah5Eva Varallyay6Research Institute for Viticulture and Oenology, Experimental Station of Kecskemet, National Research and Innovation Center, Katona Zsigmond Street 5, 6000 Kecskemet, HungaryAgricultural Biotechnology Research Institute, National Research and Innovation Center, Szent-Gyorgyi Albert Street 4, 2100 Godollo, HungaryAgricultural Biotechnology Research Institute, National Research and Innovation Center, Szent-Gyorgyi Albert Street 4, 2100 Godollo, HungaryAgricultural Biotechnology Research Institute, National Research and Innovation Center, Szent-Gyorgyi Albert Street 4, 2100 Godollo, HungaryResearch Institute for Viticulture and Oenology, Experimental Station of Kecskemet, National Research and Innovation Center, Katona Zsigmond Street 5, 6000 Kecskemet, HungaryResearch Institute for Viticulture and Oenology, Experimental Station of Kecskemet, National Research and Innovation Center, Katona Zsigmond Street 5, 6000 Kecskemet, HungaryAgricultural Biotechnology Research Institute, National Research and Innovation Center, Szent-Gyorgyi Albert Street 4, 2100 Godollo, HungaryMeristem culture and somatic embryogenesis are effective tools for virus elimination of vegetatively propagated crops including grapevine (<i>Vitis vinifera</i> L.). While both have been shown to be useful to eliminate the main grapevine viruses, their efficiency differs depending on the virus and grapevine variety. In our work, we investigated the efficiency of these two virus elimination methods using small RNA high-throughput sequencing (HTS) and RT-PCR as virus diagnostics. Field grown mother plants of four clones representing three cultivars, infected with different viruses and viroids, were selected for elimination via somatic embryogenesis (SE) and meristem culture (ME). Our results show for the first time that using SE, elimination in mother plants was effective for all viruses, i.e., grapevine rupestris vein feathering virus (GRVFV), grapevine Syrah virus 1 (GSyV-1), Grapevine virus T (GVT) and grapevine Pinot gris virus (GPGV). This study also confirms previous studies showing that SE is a possible strategy for the elimination of GFkV, GRSPaV, HSVd, and GYSVd-1. Our results demonstrate that the efficacy of virus elimination via SE is relatively high while the purging of viroids is lower. Our work provides evidence that the efficiency of SE is comparable to that of the technically difficult ME technique, and that SE will offer a more effective strategy for the production of virus-free grapevine in the future.https://www.mdpi.com/2223-7747/9/12/1782somatic embryogenesismeristem culturevirus eliminationsRNA HTS |
| spellingShingle | Mihaly Turcsan Emese Demian Tunde Varga Nikoletta Jaksa-Czotter Erno Szegedi Robert Olah Eva Varallyay HTS-Based Monitoring of the Efficiency of Somatic Embryogenesis and Meristem Cultures Used for Virus Elimination in Grapevine Plants somatic embryogenesis meristem culture virus elimination sRNA HTS |
| title | HTS-Based Monitoring of the Efficiency of Somatic Embryogenesis and Meristem Cultures Used for Virus Elimination in Grapevine |
| title_full | HTS-Based Monitoring of the Efficiency of Somatic Embryogenesis and Meristem Cultures Used for Virus Elimination in Grapevine |
| title_fullStr | HTS-Based Monitoring of the Efficiency of Somatic Embryogenesis and Meristem Cultures Used for Virus Elimination in Grapevine |
| title_full_unstemmed | HTS-Based Monitoring of the Efficiency of Somatic Embryogenesis and Meristem Cultures Used for Virus Elimination in Grapevine |
| title_short | HTS-Based Monitoring of the Efficiency of Somatic Embryogenesis and Meristem Cultures Used for Virus Elimination in Grapevine |
| title_sort | hts based monitoring of the efficiency of somatic embryogenesis and meristem cultures used for virus elimination in grapevine |
| topic | somatic embryogenesis meristem culture virus elimination sRNA HTS |
| url | https://www.mdpi.com/2223-7747/9/12/1782 |
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