Development and evaluation of ciprofloxacin-bacterial cellulose composites produced through in situ incorporation method
This paper describes the interaction and properties of bacterial cellulose (BC)–ciprofloxacin composites synthesized by in situ incorporation method. Ciprofloxacin's susceptibility to BC's producer, Acetobacter xylinum 0416, was first tested to determine its inhibitory activity against the...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-06-01
|
| Series: | Biotechnology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X22000261 |
| _version_ | 1811254135176036352 |
|---|---|
| author | Sharifah Soplah Syed Abdullah Fathin Amila Faisul Aris Siti Nur Nadhirah Said Azmi Jessica Harriette Supang Anak John Nurul Nabilah Khairul Anuar Ahmad Syafiq Fauzan Mohd Asnawi |
| author_facet | Sharifah Soplah Syed Abdullah Fathin Amila Faisul Aris Siti Nur Nadhirah Said Azmi Jessica Harriette Supang Anak John Nurul Nabilah Khairul Anuar Ahmad Syafiq Fauzan Mohd Asnawi |
| author_sort | Sharifah Soplah Syed Abdullah |
| collection | DOAJ |
| description | This paper describes the interaction and properties of bacterial cellulose (BC)–ciprofloxacin composites synthesized by in situ incorporation method. Ciprofloxacin's susceptibility to BC's producer, Acetobacter xylinum 0416, was first tested to determine its inhibitory activity against the bacteria. In situ incorporation method was performed by introducing 0.2% (w/v) ciprofloxacin into Hestrin–Schramm medium at the onset of exponential phase of A. xylinum 0416 growth. Following a 10-day incubation at 28 °C, BC–ciprofloxacin composites were harvested and further characterised, while another BC–ciprofloxacin composite was harvested and purified prior to characterisation. The interaction between ciprofloxacin and BC was proven by the presence of quinolines and fluorine groups of ciprofloxacin on unpurified BC–ciprofloxacin composite and the reduction of crystallinity index as compared to the native BC. Moreover, deposited ciprofloxacin crystals on BC film and its composition were exhibited via SEM-Energy-dispersive X-ray analysis. Unpurified BC–ciprofloxacin film was determined to have strongly inhibited the following selected diabetic foot ulcer bacteria: E. coli, K. pneumoniae and P. aeruginosa. BC has the potential to be used as a wound dressing and a carrier for ciprofloxacin. |
| first_indexed | 2024-04-12T17:01:42Z |
| format | Article |
| id | doaj.art-b9144449b7014859b548239a58ff9bf3 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-04-12T17:01:42Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-b9144449b7014859b548239a58ff9bf32022-12-22T03:24:03ZengElsevierBiotechnology Reports2215-017X2022-06-0134e00726Development and evaluation of ciprofloxacin-bacterial cellulose composites produced through in situ incorporation methodSharifah Soplah Syed Abdullah0Fathin Amila Faisul Aris1Siti Nur Nadhirah Said Azmi2Jessica Harriette Supang Anak John3Nurul Nabilah Khairul Anuar4Ahmad Syafiq Fauzan Mohd Asnawi5Section of Bioengineering Technology, Universiti Kuala Lumpur Branch Campus Malaysian Institute of Chemical and Bioengineering Technology, Lot 1988 Vendor City Taboh Naning, 78000 Alor Gajah, Melaka, Malaysia; Section of Food Engineering Technology, Universiti Kuala Lumpur Branch Campus Malaysian Institute of Chemical and Bioengineering Technology, Lot 1988 Vendor City Taboh Naning, 78000, Alor Gajah, Melaka, Malaysia; Corresponding author.Section of Bioengineering Technology, Universiti Kuala Lumpur Branch Campus Malaysian Institute of Chemical and Bioengineering Technology, Lot 1988 Vendor City Taboh Naning, 78000 Alor Gajah, Melaka, MalaysiaSection of Bioengineering Technology, Universiti Kuala Lumpur Branch Campus Malaysian Institute of Chemical and Bioengineering Technology, Lot 1988 Vendor City Taboh Naning, 78000 Alor Gajah, Melaka, MalaysiaSection of Bioengineering Technology, Universiti Kuala Lumpur Branch Campus Malaysian Institute of Chemical and Bioengineering Technology, Lot 1988 Vendor City Taboh Naning, 78000 Alor Gajah, Melaka, MalaysiaSection of Bioengineering Technology, Universiti Kuala Lumpur Branch Campus Malaysian Institute of Chemical and Bioengineering Technology, Lot 1988 Vendor City Taboh Naning, 78000 Alor Gajah, Melaka, MalaysiaSection of Chemical Engineering, Universiti Kuala Lumpur Branch Campus Malaysian Institute of Chemical and Bioengineering Technology, Lot 1988 Vendor City Taboh Naning, 78000, Alor Gajah, Melaka, MalaysiaThis paper describes the interaction and properties of bacterial cellulose (BC)–ciprofloxacin composites synthesized by in situ incorporation method. Ciprofloxacin's susceptibility to BC's producer, Acetobacter xylinum 0416, was first tested to determine its inhibitory activity against the bacteria. In situ incorporation method was performed by introducing 0.2% (w/v) ciprofloxacin into Hestrin–Schramm medium at the onset of exponential phase of A. xylinum 0416 growth. Following a 10-day incubation at 28 °C, BC–ciprofloxacin composites were harvested and further characterised, while another BC–ciprofloxacin composite was harvested and purified prior to characterisation. The interaction between ciprofloxacin and BC was proven by the presence of quinolines and fluorine groups of ciprofloxacin on unpurified BC–ciprofloxacin composite and the reduction of crystallinity index as compared to the native BC. Moreover, deposited ciprofloxacin crystals on BC film and its composition were exhibited via SEM-Energy-dispersive X-ray analysis. Unpurified BC–ciprofloxacin film was determined to have strongly inhibited the following selected diabetic foot ulcer bacteria: E. coli, K. pneumoniae and P. aeruginosa. BC has the potential to be used as a wound dressing and a carrier for ciprofloxacin.http://www.sciencedirect.com/science/article/pii/S2215017X22000261Bacterial celluloseCiprofloxacinIn situ incorporationAcetobacter xylinum |
| spellingShingle | Sharifah Soplah Syed Abdullah Fathin Amila Faisul Aris Siti Nur Nadhirah Said Azmi Jessica Harriette Supang Anak John Nurul Nabilah Khairul Anuar Ahmad Syafiq Fauzan Mohd Asnawi Development and evaluation of ciprofloxacin-bacterial cellulose composites produced through in situ incorporation method Biotechnology Reports Bacterial cellulose Ciprofloxacin In situ incorporation Acetobacter xylinum |
| title | Development and evaluation of ciprofloxacin-bacterial cellulose composites produced through in situ incorporation method |
| title_full | Development and evaluation of ciprofloxacin-bacterial cellulose composites produced through in situ incorporation method |
| title_fullStr | Development and evaluation of ciprofloxacin-bacterial cellulose composites produced through in situ incorporation method |
| title_full_unstemmed | Development and evaluation of ciprofloxacin-bacterial cellulose composites produced through in situ incorporation method |
| title_short | Development and evaluation of ciprofloxacin-bacterial cellulose composites produced through in situ incorporation method |
| title_sort | development and evaluation of ciprofloxacin bacterial cellulose composites produced through in situ incorporation method |
| topic | Bacterial cellulose Ciprofloxacin In situ incorporation Acetobacter xylinum |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X22000261 |
| work_keys_str_mv | AT sharifahsoplahsyedabdullah developmentandevaluationofciprofloxacinbacterialcellulosecompositesproducedthroughinsituincorporationmethod AT fathinamilafaisularis developmentandevaluationofciprofloxacinbacterialcellulosecompositesproducedthroughinsituincorporationmethod AT sitinurnadhirahsaidazmi developmentandevaluationofciprofloxacinbacterialcellulosecompositesproducedthroughinsituincorporationmethod AT jessicaharriettesupanganakjohn developmentandevaluationofciprofloxacinbacterialcellulosecompositesproducedthroughinsituincorporationmethod AT nurulnabilahkhairulanuar developmentandevaluationofciprofloxacinbacterialcellulosecompositesproducedthroughinsituincorporationmethod AT ahmadsyafiqfauzanmohdasnawi developmentandevaluationofciprofloxacinbacterialcellulosecompositesproducedthroughinsituincorporationmethod |