Enhanced Growth Performance Physiological and Biochemical Indexes of Trachinotus ovatus Fed With Marine Microalgae Aurantiochytrium sp. Rich in n-3 Polyunsaturated Fatty Acids
Aurantiochytrium sp. is a major source of n-3 polyunsaturated fatty acids (n-3 PUFAs), which are essential nutrients for marine fish. n-3 PUFAs have drawn increasing attention because of their great potential for improving the biological functions of fish. The growth performance and immune response...
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2020.609837/full |
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| author | Shuangfei Li Shuangfei Li Shuangfei Li Boyu Wang Boyu Wang Boyu Wang Liangxu Liu Liangxu Liu Liangxu Liu Yingjie Song Yingjie Song Yingjie Song Chuhan Lv Chuhan Lv Chuhan Lv Xingyu Zhu Xingyu Zhu Xingyu Zhu Yanqing Luo Yanqing Luo Yanqing Luo Christopher H. K. Cheng Huapu Chen Xuewei Yang Xuewei Yang Xuewei Yang Tao Li |
| author_facet | Shuangfei Li Shuangfei Li Shuangfei Li Boyu Wang Boyu Wang Boyu Wang Liangxu Liu Liangxu Liu Liangxu Liu Yingjie Song Yingjie Song Yingjie Song Chuhan Lv Chuhan Lv Chuhan Lv Xingyu Zhu Xingyu Zhu Xingyu Zhu Yanqing Luo Yanqing Luo Yanqing Luo Christopher H. K. Cheng Huapu Chen Xuewei Yang Xuewei Yang Xuewei Yang Tao Li |
| author_sort | Shuangfei Li |
| collection | DOAJ |
| description | Aurantiochytrium sp. is a major source of n-3 polyunsaturated fatty acids (n-3 PUFAs), which are essential nutrients for marine fish. n-3 PUFAs have drawn increasing attention because of their great potential for improving the biological functions of fish. The growth performance and immune response of Trachinotus ovatus were studied by applying diets with various microalgae content from 1.00 to 11.00% for 8 weeks. The results showed that, with the addition of Aurantiochytrium sp., the survival rate, weight gain rate, and specific growth rate of fish increased by a maximum of 1.02, 1.16, and 1.08 times, respectively, indicating that the intake of marine microalgae Aurantiochytrium sp. was beneficial for fish growth. As the microalgae content increased, the feed utilization efficiency index feed conversion ratio decreased (maximum 15.00%) and feed efficiency increased (maximum 1.17 times), showing that the addition of Aurantiochytrium sp. contributed to the assimilation of fish feed. Furthermore, our results showed that as the addition of Aurantiochytrium sp. increased (from 1.00 to 11.00%), the glucose content increased in the blood (from 9.04 to 27.80%). The content of fatty acids ARA, ALA, DHA, and EPA in fish was significantly increased after adding Aurantiochytrium sp. in diets. In liver, ARA content increased from 1.17 to 1.63%, ALA increased from 0.56 to 0.85%, DHA increased from 14.44 to 20.61%, and EPA increased from 1.86 to 4.40%. In muscle, ARA content increased from 0.97 to 1.24%, ALA increased from 0.59 to 0.81%, DHA increased from 14.63 to 14.82%, and EPA increased from 4.58 to 5.19%. Positive changes were observed in the blood cell count of immune related cells (white blood cells, lymphocytes, monocytes, neutrophils, and red blood cells). These results indicated that microalgae rich in n-3 PUFAs could increase the number of immune cells, thus helping to improve fish immunity and disease resistance. |
| first_indexed | 2024-12-23T23:31:29Z |
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| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-23T23:31:29Z |
| publishDate | 2021-01-01 |
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| series | Frontiers in Marine Science |
| spelling | doaj.art-b914626cf86a4d7785ef3cca2ddd3d6d2022-12-21T17:26:01ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452021-01-01710.3389/fmars.2020.609837609837Enhanced Growth Performance Physiological and Biochemical Indexes of Trachinotus ovatus Fed With Marine Microalgae Aurantiochytrium sp. Rich in n-3 Polyunsaturated Fatty AcidsShuangfei Li0Shuangfei Li1Shuangfei Li2Boyu Wang3Boyu Wang4Boyu Wang5Liangxu Liu6Liangxu Liu7Liangxu Liu8Yingjie Song9Yingjie Song10Yingjie Song11Chuhan Lv12Chuhan Lv13Chuhan Lv14Xingyu Zhu15Xingyu Zhu16Xingyu Zhu17Yanqing Luo18Yanqing Luo19Yanqing Luo20Christopher H. K. Cheng21Huapu Chen22Xuewei Yang23Xuewei Yang24Xuewei Yang25Tao Li26Guangdong Key Laboratory of Plant Epigenetics, Guangdong Technology Research Center for Marine Algal Bioengineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Marine Biological Resources and Ecology Environment, Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaLonghua Innovation Institute for Biotechnology, Shenzhen University, Shenzhen, ChinaGuangdong Key Laboratory of Plant Epigenetics, Guangdong Technology Research Center for Marine Algal Bioengineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Marine Biological Resources and Ecology Environment, Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaLonghua Innovation Institute for Biotechnology, Shenzhen University, Shenzhen, ChinaGuangdong Key Laboratory of Plant Epigenetics, Guangdong Technology Research Center for Marine Algal Bioengineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Marine Biological Resources and Ecology Environment, Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaLonghua Innovation Institute for Biotechnology, Shenzhen University, Shenzhen, ChinaGuangdong Key Laboratory of Plant Epigenetics, Guangdong Technology Research Center for Marine Algal Bioengineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Marine Biological Resources and Ecology Environment, Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaLonghua Innovation Institute for Biotechnology, Shenzhen University, Shenzhen, ChinaGuangdong Key Laboratory of Plant Epigenetics, Guangdong Technology Research Center for Marine Algal Bioengineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Marine Biological Resources and Ecology Environment, Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaLonghua Innovation Institute for Biotechnology, Shenzhen University, Shenzhen, ChinaGuangdong Key Laboratory of Plant Epigenetics, Guangdong Technology Research Center for Marine Algal Bioengineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Marine Biological Resources and Ecology Environment, Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaLonghua Innovation Institute for Biotechnology, Shenzhen University, Shenzhen, ChinaGuangdong Key Laboratory of Plant Epigenetics, Guangdong Technology Research Center for Marine Algal Bioengineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Marine Biological Resources and Ecology Environment, Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaLonghua Innovation Institute for Biotechnology, Shenzhen University, Shenzhen, ChinaSchool of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, ChinaGuangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Fisheries College, Guangdong Ocean University, Zhanjiang, ChinaGuangdong Key Laboratory of Plant Epigenetics, Guangdong Technology Research Center for Marine Algal Bioengineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Marine Biological Resources and Ecology Environment, Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaLonghua Innovation Institute for Biotechnology, Shenzhen University, Shenzhen, ChinaShenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen, ChinaAurantiochytrium sp. is a major source of n-3 polyunsaturated fatty acids (n-3 PUFAs), which are essential nutrients for marine fish. n-3 PUFAs have drawn increasing attention because of their great potential for improving the biological functions of fish. The growth performance and immune response of Trachinotus ovatus were studied by applying diets with various microalgae content from 1.00 to 11.00% for 8 weeks. The results showed that, with the addition of Aurantiochytrium sp., the survival rate, weight gain rate, and specific growth rate of fish increased by a maximum of 1.02, 1.16, and 1.08 times, respectively, indicating that the intake of marine microalgae Aurantiochytrium sp. was beneficial for fish growth. As the microalgae content increased, the feed utilization efficiency index feed conversion ratio decreased (maximum 15.00%) and feed efficiency increased (maximum 1.17 times), showing that the addition of Aurantiochytrium sp. contributed to the assimilation of fish feed. Furthermore, our results showed that as the addition of Aurantiochytrium sp. increased (from 1.00 to 11.00%), the glucose content increased in the blood (from 9.04 to 27.80%). The content of fatty acids ARA, ALA, DHA, and EPA in fish was significantly increased after adding Aurantiochytrium sp. in diets. In liver, ARA content increased from 1.17 to 1.63%, ALA increased from 0.56 to 0.85%, DHA increased from 14.44 to 20.61%, and EPA increased from 1.86 to 4.40%. In muscle, ARA content increased from 0.97 to 1.24%, ALA increased from 0.59 to 0.81%, DHA increased from 14.63 to 14.82%, and EPA increased from 4.58 to 5.19%. Positive changes were observed in the blood cell count of immune related cells (white blood cells, lymphocytes, monocytes, neutrophils, and red blood cells). These results indicated that microalgae rich in n-3 PUFAs could increase the number of immune cells, thus helping to improve fish immunity and disease resistance.https://www.frontiersin.org/articles/10.3389/fmars.2020.609837/fulln-3 polyunsaturated fatty acidsdocosahexaenoic acidpolyunsaturated fatty acidsAurantiochytrium sp.Trachinotus ovatus |
| spellingShingle | Shuangfei Li Shuangfei Li Shuangfei Li Boyu Wang Boyu Wang Boyu Wang Liangxu Liu Liangxu Liu Liangxu Liu Yingjie Song Yingjie Song Yingjie Song Chuhan Lv Chuhan Lv Chuhan Lv Xingyu Zhu Xingyu Zhu Xingyu Zhu Yanqing Luo Yanqing Luo Yanqing Luo Christopher H. K. Cheng Huapu Chen Xuewei Yang Xuewei Yang Xuewei Yang Tao Li Enhanced Growth Performance Physiological and Biochemical Indexes of Trachinotus ovatus Fed With Marine Microalgae Aurantiochytrium sp. Rich in n-3 Polyunsaturated Fatty Acids Frontiers in Marine Science n-3 polyunsaturated fatty acids docosahexaenoic acid polyunsaturated fatty acids Aurantiochytrium sp. Trachinotus ovatus |
| title | Enhanced Growth Performance Physiological and Biochemical Indexes of Trachinotus ovatus Fed With Marine Microalgae Aurantiochytrium sp. Rich in n-3 Polyunsaturated Fatty Acids |
| title_full | Enhanced Growth Performance Physiological and Biochemical Indexes of Trachinotus ovatus Fed With Marine Microalgae Aurantiochytrium sp. Rich in n-3 Polyunsaturated Fatty Acids |
| title_fullStr | Enhanced Growth Performance Physiological and Biochemical Indexes of Trachinotus ovatus Fed With Marine Microalgae Aurantiochytrium sp. Rich in n-3 Polyunsaturated Fatty Acids |
| title_full_unstemmed | Enhanced Growth Performance Physiological and Biochemical Indexes of Trachinotus ovatus Fed With Marine Microalgae Aurantiochytrium sp. Rich in n-3 Polyunsaturated Fatty Acids |
| title_short | Enhanced Growth Performance Physiological and Biochemical Indexes of Trachinotus ovatus Fed With Marine Microalgae Aurantiochytrium sp. Rich in n-3 Polyunsaturated Fatty Acids |
| title_sort | enhanced growth performance physiological and biochemical indexes of trachinotus ovatus fed with marine microalgae aurantiochytrium sp rich in n 3 polyunsaturated fatty acids |
| topic | n-3 polyunsaturated fatty acids docosahexaenoic acid polyunsaturated fatty acids Aurantiochytrium sp. Trachinotus ovatus |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2020.609837/full |
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