Tumour Associated Tissue Eosinophilia in Oral Squamous Cell Carcinoma by Special Histochemical Analysis of Tissue Eosinophilia using Congo Red Staining
Introduction: Tumour Associated Tissue Eosinophilia (TATE) is an important phenomenon occurring in the tumour microenvironment and has a pivotal role. TATE is defined as “eosinophilic stromal infiltration of a tumour not associated with tumour necrosis or ulceration”. The exact role of TATE in m...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Pvt. Ltd.
2022-04-01
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| Series: | National Journal of Laboratory Medicine |
| Subjects: | |
| Online Access: | https://www.njlm.net/articles/PDF/2617/53615_final_F(SHU)_PF1(AG_KM)_PFA(KM)_PB(AG_SHU)_PN(SHU).pdf |
| Summary: | Introduction: Tumour Associated Tissue Eosinophilia (TATE) is an
important phenomenon occurring in the tumour microenvironment
and has a pivotal role. TATE is defined as “eosinophilic stromal
infiltration of a tumour not associated with tumour necrosis or
ulceration”. The exact role of TATE in malignancies is yet unclear but
studies have shown that TATE usually has a favourable outcome in
head and neck Squamous Cell Carcinoma (SCC). Therefore, more
studies are needed to substantiate this data.
Aim: To compare TATE between normal epithelium and Oral
Squamous Cell Carcinoma (OSCC) and compare TATE between
the histological grades of OSCC.
Materials and Methods: A retrospective study was conducted
at Dr. BR Ambedkar Medical College, Bangalore, Karnataka,
India, from June 2019-January 2021 which included 50 cases,
10 from normal mucosa and 40 histopathologically diagnosed
cases of OSCC. 1% congo red solution was used to stain
4 µm thick sections. The sections were examined at a high
magnification (40X) and 10 microscopic fields were examined in
succession for eosinophils. The average number of eosinophils
observed were compared using univariate analysis, which
included the Analysis of Variance (ANOVA) test and the Chisquare test and a p-value of 0.001 was considered statistically
significant.
Results: The mean value of tissue eosinophils increased in
OSCC compared to normal mucosa, according to present
study findings. When comparing different grades of carcinoma,
statistical analysis revealed that well differentiated squamous
cell carcinoma had a greater TATE than other grades, which was
statistically significant (p-value <0.001).
Conclusion: The higher eosinophil count in well differentiated
OSCC compared to the other grades could be associated with
a better clinical outcome for the patient. In OSCC, TATE can be
utilised as a predictor of a good prognosis. |
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| ISSN: | 2277-8551 2455-6882 |