The Protective Effects of Thymosin-β-4 in a Rat Model of Ischemic Acute Kidney Injury
Background Despite the progress in the treatment of acute kidney injury (AKI), current curative approaches fail to provide adequate treatment. In this study, we aimed to investigate the possible protective effects of thymosin-β-4(Tβ4) on an ischemic AKI model in rats. Methods Rats were randomly assi...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-07-01
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| Series: | Journal of Investigative Surgery |
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| Online Access: | http://dx.doi.org/10.1080/08941939.2019.1672841 |
| _version_ | 1827817377382793216 |
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| author | Ugur Aksu Onur M. Yaman Ibrahim Guner Gulcan Guntas Fuat Sonmez Gamze Tanriverdi Mediha Eser Aris Cakiris Sibel Akyol İsmail Seçkin Hafize Uzun Nermin Yelmen Gulderen Sahin |
| author_facet | Ugur Aksu Onur M. Yaman Ibrahim Guner Gulcan Guntas Fuat Sonmez Gamze Tanriverdi Mediha Eser Aris Cakiris Sibel Akyol İsmail Seçkin Hafize Uzun Nermin Yelmen Gulderen Sahin |
| author_sort | Ugur Aksu |
| collection | DOAJ |
| description | Background Despite the progress in the treatment of acute kidney injury (AKI), current curative approaches fail to provide adequate treatment. In this study, we aimed to investigate the possible protective effects of thymosin-β-4(Tβ4) on an ischemic AKI model in rats. Methods Rats were randomly assigned into four groups (n = 8/group): The control group (sham-operated), the ischemia-reperfusion (I/R) group; renal ischemia (90 min) by infrarenal abdominal aortic occlusion followed by reperfusion (3 h), the Tβ4 + I/R group; treated with Tβ4 before I/R, and the I/Tβ4/R group; treated with Tβ4 just before reperfusion. Besides renal function determination (creatinine (Cr) and blood urea nitrogen (BUN)); histological evaluation was also conducted. Renal tissue caspase-9, matrix metalloproteinase (MMP-9) activities, and hyaluronan levels were measured. Additionally, renal tissue oxidative stress (lipid hydroperoxide, malondialdehyde, superoxide dismutase, glutathione, pro-oxidant-antioxidant balance, ferric reducing antioxidant power, nitric oxide), inflammation (tumor necrosis factor-α, interleukin-1β, interleukin-6, nuclear factor-κβ) were evaluated. Results I/R increased the level of caspase-9, MMP-9 activity, and hyaluronan (p < 0.001) and these were significantly decreased in both Tβ4 groups. Moreover, I/R led to increases in oxidative stress and inflammation parameters (p < 0.001) while the levels of antioxidants were decreased. Nevertheless, Tβ4 in both groups were able to restore oxidative stress and inflammation parameters. Furthermore, Tβ4 attenuated histologic injury caused by I/R (p < 0.01) and diminished serum urea-creatinine levels (p < 0.001). Conclusion These results suggest that Tβ4 has significant improving effects in ischemic acute kidney injury. This beneficial effect might be a result of the inhibition of extracellular matrix remodeling and apoptosis cascade via modulation in renal redox status and inflammation. |
| first_indexed | 2024-03-12T00:33:25Z |
| format | Article |
| id | doaj.art-b918bc840de24c0da02c038023809b91 |
| institution | Directory Open Access Journal |
| issn | 0894-1939 1521-0553 |
| language | English |
| last_indexed | 2024-03-12T00:33:25Z |
| publishDate | 2021-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Investigative Surgery |
| spelling | doaj.art-b918bc840de24c0da02c038023809b912023-09-15T10:07:31ZengTaylor & Francis GroupJournal of Investigative Surgery0894-19391521-05532021-07-0134660160910.1080/08941939.2019.16728411672841The Protective Effects of Thymosin-β-4 in a Rat Model of Ischemic Acute Kidney InjuryUgur Aksu0Onur M. Yaman1Ibrahim Guner2Gulcan Guntas3Fuat Sonmez4Gamze Tanriverdi5Mediha Eser6Aris Cakiris7Sibel Akyol8İsmail Seçkin9Hafize Uzun10Nermin Yelmen11Gulderen Sahin12Department of Biology, Science Faculty, Istanbul UniversityDepartment of Physiology, Cerrahpasa Medical Faculty, Istanbul University-CerrahpasaDepartment of Physiology, Cerrahpasa Medical Faculty, Istanbul University-CerrahpasaDepartment of Nursery, School of Health, University of KırklareliDepartment of Physiology, Cerrahpasa Medical Faculty, Istanbul University-CerrahpasaDepartment of Histology and Embriology, Cerrahpasa Medical Faculty, Istanbul University-CerrahpasaDepartment of Histology and Embriology, Cerrahpasa Medical Faculty, Istanbul University-CerrahpasaInstitute of Health Sciences, Genetics, Istanbul UniversityDepartment of Physiology, Cerrahpasa Medical Faculty, Istanbul University-CerrahpasaDepartment of Histology and Embriology, Cerrahpasa Medical Faculty, Istanbul University-CerrahpasaDepartment of Biochemistry, Cerrahpasa Medical Faculty, Istanbul University-CerrahpasaDepartment of Physiology, Cerrahpasa Medical Faculty, Istanbul University-CerrahpasaDepartment of Physiology, Cerrahpasa Medical Faculty, Istanbul University-CerrahpasaBackground Despite the progress in the treatment of acute kidney injury (AKI), current curative approaches fail to provide adequate treatment. In this study, we aimed to investigate the possible protective effects of thymosin-β-4(Tβ4) on an ischemic AKI model in rats. Methods Rats were randomly assigned into four groups (n = 8/group): The control group (sham-operated), the ischemia-reperfusion (I/R) group; renal ischemia (90 min) by infrarenal abdominal aortic occlusion followed by reperfusion (3 h), the Tβ4 + I/R group; treated with Tβ4 before I/R, and the I/Tβ4/R group; treated with Tβ4 just before reperfusion. Besides renal function determination (creatinine (Cr) and blood urea nitrogen (BUN)); histological evaluation was also conducted. Renal tissue caspase-9, matrix metalloproteinase (MMP-9) activities, and hyaluronan levels were measured. Additionally, renal tissue oxidative stress (lipid hydroperoxide, malondialdehyde, superoxide dismutase, glutathione, pro-oxidant-antioxidant balance, ferric reducing antioxidant power, nitric oxide), inflammation (tumor necrosis factor-α, interleukin-1β, interleukin-6, nuclear factor-κβ) were evaluated. Results I/R increased the level of caspase-9, MMP-9 activity, and hyaluronan (p < 0.001) and these were significantly decreased in both Tβ4 groups. Moreover, I/R led to increases in oxidative stress and inflammation parameters (p < 0.001) while the levels of antioxidants were decreased. Nevertheless, Tβ4 in both groups were able to restore oxidative stress and inflammation parameters. Furthermore, Tβ4 attenuated histologic injury caused by I/R (p < 0.01) and diminished serum urea-creatinine levels (p < 0.001). Conclusion These results suggest that Tβ4 has significant improving effects in ischemic acute kidney injury. This beneficial effect might be a result of the inhibition of extracellular matrix remodeling and apoptosis cascade via modulation in renal redox status and inflammation.http://dx.doi.org/10.1080/08941939.2019.1672841thymosin-β-4acute kidney injuryischemia |
| spellingShingle | Ugur Aksu Onur M. Yaman Ibrahim Guner Gulcan Guntas Fuat Sonmez Gamze Tanriverdi Mediha Eser Aris Cakiris Sibel Akyol İsmail Seçkin Hafize Uzun Nermin Yelmen Gulderen Sahin The Protective Effects of Thymosin-β-4 in a Rat Model of Ischemic Acute Kidney Injury Journal of Investigative Surgery thymosin-β-4 acute kidney injury ischemia |
| title | The Protective Effects of Thymosin-β-4 in a Rat Model of Ischemic Acute Kidney Injury |
| title_full | The Protective Effects of Thymosin-β-4 in a Rat Model of Ischemic Acute Kidney Injury |
| title_fullStr | The Protective Effects of Thymosin-β-4 in a Rat Model of Ischemic Acute Kidney Injury |
| title_full_unstemmed | The Protective Effects of Thymosin-β-4 in a Rat Model of Ischemic Acute Kidney Injury |
| title_short | The Protective Effects of Thymosin-β-4 in a Rat Model of Ischemic Acute Kidney Injury |
| title_sort | protective effects of thymosin β 4 in a rat model of ischemic acute kidney injury |
| topic | thymosin-β-4 acute kidney injury ischemia |
| url | http://dx.doi.org/10.1080/08941939.2019.1672841 |
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