Prospects for Fungal Bioremediation of Acidic Radioactive Waste Sites: Characterization and Genome Sequence of Rhodotorula taiwanensis MD1149
Highly concentrated radionuclide waste produced during the Cold War era is stored at US Department of Energy (DOE) production sites. This radioactive waste was often highly acidic and mixed with heavy metals, and has been leaking into the environment since the 1950s. Because of the danger and expens...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02528/full |
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| author | Rok Tkavc Rok Tkavc Vera Y. Matrosova Vera Y. Matrosova Olga E. Grichenko Olga E. Grichenko Cene Gostinčar Robert P. Volpe Robert P. Volpe Polina Klimenkova Polina Klimenkova Elena K. Gaidamakova Elena K. Gaidamakova Carol E. Zhou Benjamin J. Stewart Mathew G. Lyman Stephanie A. Malfatti Bonnee Rubinfeld Melanie Courtot Jatinder Singh Clifton L. Dalgard Clifton L. Dalgard Theron Hamilton Kenneth G. Frey Nina Gunde-Cimerman Lawrence Dugan Michael J. Daly |
| author_facet | Rok Tkavc Rok Tkavc Vera Y. Matrosova Vera Y. Matrosova Olga E. Grichenko Olga E. Grichenko Cene Gostinčar Robert P. Volpe Robert P. Volpe Polina Klimenkova Polina Klimenkova Elena K. Gaidamakova Elena K. Gaidamakova Carol E. Zhou Benjamin J. Stewart Mathew G. Lyman Stephanie A. Malfatti Bonnee Rubinfeld Melanie Courtot Jatinder Singh Clifton L. Dalgard Clifton L. Dalgard Theron Hamilton Kenneth G. Frey Nina Gunde-Cimerman Lawrence Dugan Michael J. Daly |
| author_sort | Rok Tkavc |
| collection | DOAJ |
| description | Highly concentrated radionuclide waste produced during the Cold War era is stored at US Department of Energy (DOE) production sites. This radioactive waste was often highly acidic and mixed with heavy metals, and has been leaking into the environment since the 1950s. Because of the danger and expense of cleanup of such radioactive sites by physicochemical processes, in situ bioremediation methods are being developed for cleanup of contaminated ground and groundwater. To date, the most developed microbial treatment proposed for high-level radioactive sites employs the radiation-resistant bacterium Deinococcus radiodurans. However, the use of Deinococcus spp. and other bacteria is limited by their sensitivity to low pH. We report the characterization of 27 diverse environmental yeasts for their resistance to ionizing radiation (chronic and acute), heavy metals, pH minima, temperature maxima and optima, and their ability to form biofilms. Remarkably, many yeasts are extremely resistant to ionizing radiation and heavy metals. They also excrete carboxylic acids and are exceptionally tolerant to low pH. A special focus is placed on Rhodotorula taiwanensis MD1149, which was the most resistant to acid and gamma radiation. MD1149 is capable of growing under 66 Gy/h at pH 2.3 and in the presence of high concentrations of mercury and chromium compounds, and forming biofilms under high-level chronic radiation and low pH. We present the whole genome sequence and annotation of R. taiwanensis strain MD1149, with a comparison to other Rhodotorula species. This survey elevates yeasts to the frontier of biology's most radiation-resistant representatives, presenting a strong rationale for a role of fungi in bioremediation of acidic radioactive waste sites. |
| first_indexed | 2024-04-12T00:40:58Z |
| format | Article |
| id | doaj.art-b919e934934f4f2cb0d915ffeb94ff35 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T00:40:58Z |
| publishDate | 2018-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-b919e934934f4f2cb0d915ffeb94ff352022-12-22T03:55:02ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-01-01810.3389/fmicb.2017.02528305317Prospects for Fungal Bioremediation of Acidic Radioactive Waste Sites: Characterization and Genome Sequence of Rhodotorula taiwanensis MD1149Rok Tkavc0Rok Tkavc1Vera Y. Matrosova2Vera Y. Matrosova3Olga E. Grichenko4Olga E. Grichenko5Cene Gostinčar6Robert P. Volpe7Robert P. Volpe8Polina Klimenkova9Polina Klimenkova10Elena K. Gaidamakova11Elena K. Gaidamakova12Carol E. Zhou13Benjamin J. Stewart14Mathew G. Lyman15Stephanie A. Malfatti16Bonnee Rubinfeld17Melanie Courtot18Jatinder Singh19Clifton L. Dalgard20Clifton L. Dalgard21Theron Hamilton22Kenneth G. Frey23Nina Gunde-Cimerman24Lawrence Dugan25Michael J. Daly26Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD, United StatesHenry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United StatesDepartment of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD, United StatesHenry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United StatesDepartment of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD, United StatesHenry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United StatesDepartment of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, SloveniaDepartment of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD, United StatesHenry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United StatesDepartment of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD, United StatesHenry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United StatesDepartment of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD, United StatesHenry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United StatesLawrence Livermore National Laboratory, Computing Applications and Research Department, Livermore, CA, United StatesBiosciences and Biotechnology Division, Physics and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, United StatesBiosciences and Biotechnology Division, Physics and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, United StatesBiosciences and Biotechnology Division, Physics and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, United StatesBiosciences and Biotechnology Division, Physics and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, United StatesEuropean Molecular Biology Laboratory, European Bioinformatics Institute, Cambridge, United KingdomCollaborative Health Initiative Research Program, Uniformed Services University of the Health Sciences, Bethesda, MD, United StatesDepartment of Anatomy, Physiology and Genetics, Uniformed Services University of the Health Sciences, Bethesda, MD, United StatesThe American Genome Center, Bethesda, MD, United States0Biological Defense Research Directorate, Naval Medical Research Center, Fredrick, MD, United States0Biological Defense Research Directorate, Naval Medical Research Center, Fredrick, MD, United StatesDepartment of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, SloveniaBiosciences and Biotechnology Division, Physics and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, United StatesDepartment of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD, United StatesHighly concentrated radionuclide waste produced during the Cold War era is stored at US Department of Energy (DOE) production sites. This radioactive waste was often highly acidic and mixed with heavy metals, and has been leaking into the environment since the 1950s. Because of the danger and expense of cleanup of such radioactive sites by physicochemical processes, in situ bioremediation methods are being developed for cleanup of contaminated ground and groundwater. To date, the most developed microbial treatment proposed for high-level radioactive sites employs the radiation-resistant bacterium Deinococcus radiodurans. However, the use of Deinococcus spp. and other bacteria is limited by their sensitivity to low pH. We report the characterization of 27 diverse environmental yeasts for their resistance to ionizing radiation (chronic and acute), heavy metals, pH minima, temperature maxima and optima, and their ability to form biofilms. Remarkably, many yeasts are extremely resistant to ionizing radiation and heavy metals. They also excrete carboxylic acids and are exceptionally tolerant to low pH. A special focus is placed on Rhodotorula taiwanensis MD1149, which was the most resistant to acid and gamma radiation. MD1149 is capable of growing under 66 Gy/h at pH 2.3 and in the presence of high concentrations of mercury and chromium compounds, and forming biofilms under high-level chronic radiation and low pH. We present the whole genome sequence and annotation of R. taiwanensis strain MD1149, with a comparison to other Rhodotorula species. This survey elevates yeasts to the frontier of biology's most radiation-resistant representatives, presenting a strong rationale for a role of fungi in bioremediation of acidic radioactive waste sites.http://journal.frontiersin.org/article/10.3389/fmicb.2017.02528/fullbioremediationyeastsradiation resistanceheavy metal resistancepH minimumtemperature maximum |
| spellingShingle | Rok Tkavc Rok Tkavc Vera Y. Matrosova Vera Y. Matrosova Olga E. Grichenko Olga E. Grichenko Cene Gostinčar Robert P. Volpe Robert P. Volpe Polina Klimenkova Polina Klimenkova Elena K. Gaidamakova Elena K. Gaidamakova Carol E. Zhou Benjamin J. Stewart Mathew G. Lyman Stephanie A. Malfatti Bonnee Rubinfeld Melanie Courtot Jatinder Singh Clifton L. Dalgard Clifton L. Dalgard Theron Hamilton Kenneth G. Frey Nina Gunde-Cimerman Lawrence Dugan Michael J. Daly Prospects for Fungal Bioremediation of Acidic Radioactive Waste Sites: Characterization and Genome Sequence of Rhodotorula taiwanensis MD1149 Frontiers in Microbiology bioremediation yeasts radiation resistance heavy metal resistance pH minimum temperature maximum |
| title | Prospects for Fungal Bioremediation of Acidic Radioactive Waste Sites: Characterization and Genome Sequence of Rhodotorula taiwanensis MD1149 |
| title_full | Prospects for Fungal Bioremediation of Acidic Radioactive Waste Sites: Characterization and Genome Sequence of Rhodotorula taiwanensis MD1149 |
| title_fullStr | Prospects for Fungal Bioremediation of Acidic Radioactive Waste Sites: Characterization and Genome Sequence of Rhodotorula taiwanensis MD1149 |
| title_full_unstemmed | Prospects for Fungal Bioremediation of Acidic Radioactive Waste Sites: Characterization and Genome Sequence of Rhodotorula taiwanensis MD1149 |
| title_short | Prospects for Fungal Bioremediation of Acidic Radioactive Waste Sites: Characterization and Genome Sequence of Rhodotorula taiwanensis MD1149 |
| title_sort | prospects for fungal bioremediation of acidic radioactive waste sites characterization and genome sequence of rhodotorula taiwanensis md1149 |
| topic | bioremediation yeasts radiation resistance heavy metal resistance pH minimum temperature maximum |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02528/full |
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