Isolation and characterization of an ERF-B3 gene associated with flower abnormalities in non-heading Chinese cabbage
BrcERF-B3 gene, a member of ethylene-responsive factor family, was screened from a mutant plant in non-heading Chinese cabbage (Brassica rapa ssp. chinensis) by cDNA-AFLP technology. We got full length cDNA of two BrcERF-B3 genes by homology-based cloning from two materials and found that their nucl...
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Elsevier
2016-03-01
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Series: | Journal of Integrative Agriculture |
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author | Yu-chao XU Xi-lin HOU Wei-wei XU Lu-lu SHEN Shan-wu LÜ Shi-lin ZHANG Chun-mei HU |
author_facet | Yu-chao XU Xi-lin HOU Wei-wei XU Lu-lu SHEN Shan-wu LÜ Shi-lin ZHANG Chun-mei HU |
author_sort | Yu-chao XU |
collection | DOAJ |
description | BrcERF-B3 gene, a member of ethylene-responsive factor family, was screened from a mutant plant in non-heading Chinese cabbage (Brassica rapa ssp. chinensis) by cDNA-AFLP technology. We got full length cDNA of two BrcERF-B3 genes by homology-based cloning from two materials and found that their nucleotide sequences were the same by sequencing. The BrcERF-B3 protein, belonging to the B3 subgroup of the ERF subfamily, shared a close relationship with B. rapa. RT-PCR result showed that BrcERF-B3 expressed only in mutant stamen rather than maintainer stamen. qRT-PCR results indicated that BrcERF-B3 expressed highly during reproductive growth development and in the early of mutant buds, suggesting BrcERF-B3 might be involved in the formation of abnormal flower in mutant. What's more, the expression of BrcERF-B3 was more significant to ABA, MeJA and cold stresses in mutant than in maintainer and was down-regulated in NaCl treatment in two lines, implying BrcERF-B3 might be different roles in biotic and abiotic stresses. |
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issn | 2095-3119 |
language | English |
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spelling | doaj.art-b91f8d1c55604b7686031909c05b58ea2022-12-21T22:11:45ZengElsevierJournal of Integrative Agriculture2095-31192016-03-01153528536Isolation and characterization of an ERF-B3 gene associated with flower abnormalities in non-heading Chinese cabbageYu-chao XU0Xi-lin HOU1Wei-wei XU2Lu-lu SHEN3Shan-wu LÜ4Shi-lin ZHANG5Chun-mei HU6State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/College of Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/College of Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/College of Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaAgriculture Committee of Feixi County, Hefei 230001, P.R. ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/College of Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/College of Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/College of Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R. China; Correspondence HU Chun-mei, Tel: +86-25-84395756BrcERF-B3 gene, a member of ethylene-responsive factor family, was screened from a mutant plant in non-heading Chinese cabbage (Brassica rapa ssp. chinensis) by cDNA-AFLP technology. We got full length cDNA of two BrcERF-B3 genes by homology-based cloning from two materials and found that their nucleotide sequences were the same by sequencing. The BrcERF-B3 protein, belonging to the B3 subgroup of the ERF subfamily, shared a close relationship with B. rapa. RT-PCR result showed that BrcERF-B3 expressed only in mutant stamen rather than maintainer stamen. qRT-PCR results indicated that BrcERF-B3 expressed highly during reproductive growth development and in the early of mutant buds, suggesting BrcERF-B3 might be involved in the formation of abnormal flower in mutant. What's more, the expression of BrcERF-B3 was more significant to ABA, MeJA and cold stresses in mutant than in maintainer and was down-regulated in NaCl treatment in two lines, implying BrcERF-B3 might be different roles in biotic and abiotic stresses.http://www.sciencedirect.com/science/article/pii/S2095311916616337non-heading Chinese cabbagestamen-petalodyethylene-responsive factorgene expression |
spellingShingle | Yu-chao XU Xi-lin HOU Wei-wei XU Lu-lu SHEN Shan-wu LÜ Shi-lin ZHANG Chun-mei HU Isolation and characterization of an ERF-B3 gene associated with flower abnormalities in non-heading Chinese cabbage Journal of Integrative Agriculture non-heading Chinese cabbage stamen-petalody ethylene-responsive factor gene expression |
title | Isolation and characterization of an ERF-B3 gene associated with flower abnormalities in non-heading Chinese cabbage |
title_full | Isolation and characterization of an ERF-B3 gene associated with flower abnormalities in non-heading Chinese cabbage |
title_fullStr | Isolation and characterization of an ERF-B3 gene associated with flower abnormalities in non-heading Chinese cabbage |
title_full_unstemmed | Isolation and characterization of an ERF-B3 gene associated with flower abnormalities in non-heading Chinese cabbage |
title_short | Isolation and characterization of an ERF-B3 gene associated with flower abnormalities in non-heading Chinese cabbage |
title_sort | isolation and characterization of an erf b3 gene associated with flower abnormalities in non heading chinese cabbage |
topic | non-heading Chinese cabbage stamen-petalody ethylene-responsive factor gene expression |
url | http://www.sciencedirect.com/science/article/pii/S2095311916616337 |
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