Identification of miRnas with possible prognostic roles for HAM/TSP
ABSTRACTHuman T-cell lymphotropic virus type 1 (HTLV-1)-associated myelopathy/tropic spastic paraparesis (HAM/TSP) is an insidiously progressive spinal cord disease for which there is no effective treatment. There is great interest in developing potential biomarkers to predict the pathogenesis of HA...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Virulence |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2023.2230015 |
| _version_ | 1827391580239036416 |
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| author | Daniela Raguer Valadão de Souza Rodrigo Pessôa Youko Nukui Juliana Pereira Rosa Nascimento Marcusso Augusto César Penalva de Oliveira Jorge Casseb Alberto José da Silva Duarte Patricia Bianca Clissa Sabri Saeed Sanabani |
| author_facet | Daniela Raguer Valadão de Souza Rodrigo Pessôa Youko Nukui Juliana Pereira Rosa Nascimento Marcusso Augusto César Penalva de Oliveira Jorge Casseb Alberto José da Silva Duarte Patricia Bianca Clissa Sabri Saeed Sanabani |
| author_sort | Daniela Raguer Valadão de Souza |
| collection | DOAJ |
| description | ABSTRACTHuman T-cell lymphotropic virus type 1 (HTLV-1)-associated myelopathy/tropic spastic paraparesis (HAM/TSP) is an insidiously progressive spinal cord disease for which there is no effective treatment. There is great interest in developing potential biomarkers to predict the pathogenesis of HAM/TSP disease. In this study, Illumina Massive Parallel Sequencing (MPS) technology was used to investigate the cellular global noncoding RNAome expression profile in HAM/TSP patients (n = 10), asymptomatic HTLV-1-infected carriers (ASP, n = 8), and a second group of healthy controls (n = 5). Various bioinformatics tools were used to align, annotate, and profile the sRNA-MPS reads. Among the 402 sRNAs detected, 251 were known and 50 were potentially novel sRNAs in the HAM and ASP groups compared with the HC group. Sixty-eight known sRNAs were significantly different between the ASP and HAM groups. Eighty-eight mature miRNAs were downregulated in subjects from HAM compared with ASP. Three of these miRs (hsa-miR-185-5p, 32-5p, and 192-5p) have the potential to be used as biomarkers for predicting the pathogenesis of HAM/TSP. The seven most deregulated miRs target genes have been associated with a variety of biological processes and molecular functions. The reactome pathways relevant to our findings provide a rich source of data and offer the opportunity to better understand sRNA regulation and function in HTLV-1 pathophysiology. To the best of our knowledge, this study is the first to demonstrate evaluates sRNAs in HTLV-1 patients with HAM/TSP. |
| first_indexed | 2024-03-08T17:14:09Z |
| format | Article |
| id | doaj.art-b9243f796bdc4703b65b1029bcfe078d |
| institution | Directory Open Access Journal |
| issn | 2150-5594 2150-5608 |
| language | English |
| last_indexed | 2024-03-08T17:14:09Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj.art-b9243f796bdc4703b65b1029bcfe078d2024-01-03T17:26:58ZengTaylor & Francis GroupVirulence2150-55942150-56082023-12-0114110.1080/21505594.2023.2230015Identification of miRnas with possible prognostic roles for HAM/TSPDaniela Raguer Valadão de Souza0Rodrigo Pessôa1Youko Nukui2Juliana Pereira3Rosa Nascimento Marcusso4Augusto César Penalva de Oliveira5Jorge Casseb6Alberto José da Silva Duarte7Patricia Bianca Clissa8Sabri Saeed Sanabani9Postgraduate Program in Translational Medicine, Department of Medicine, Federal University of São Paulo (UNIFESP), São Paulo, BrazilPostgraduate Program in Translational Medicine, Department of Medicine, Federal University of São Paulo (UNIFESP), São Paulo, BrazilDepartment of Hematology, Faculty of Medicine, University of São Paulo, São Paulo, BrazilDepartment of Hematology, Faculty of Medicine, University of São Paulo, São Paulo, BrazilDepartment of Neurology, Emilio Ribas Institute of Infectious Diseases, São Paulo, BrazilDepartment of Neurology, Emilio Ribas Institute of Infectious Diseases, São Paulo, BrazilLaboratory of Medical Investigation LIM-56, Division of Dermatology, University of Sao Paulo, Sao Paulo, BrazilLaboratory of Medical Investigation LIM-56, Division of Dermatology, University of Sao Paulo, Sao Paulo, BrazilImmunopathology Laboratory, Butantan Institute, São Paulo, BrazilLaboratory of Medical Investigation LIM-56, Division of Dermatology, University of Sao Paulo, Sao Paulo, BrazilABSTRACTHuman T-cell lymphotropic virus type 1 (HTLV-1)-associated myelopathy/tropic spastic paraparesis (HAM/TSP) is an insidiously progressive spinal cord disease for which there is no effective treatment. There is great interest in developing potential biomarkers to predict the pathogenesis of HAM/TSP disease. In this study, Illumina Massive Parallel Sequencing (MPS) technology was used to investigate the cellular global noncoding RNAome expression profile in HAM/TSP patients (n = 10), asymptomatic HTLV-1-infected carriers (ASP, n = 8), and a second group of healthy controls (n = 5). Various bioinformatics tools were used to align, annotate, and profile the sRNA-MPS reads. Among the 402 sRNAs detected, 251 were known and 50 were potentially novel sRNAs in the HAM and ASP groups compared with the HC group. Sixty-eight known sRNAs were significantly different between the ASP and HAM groups. Eighty-eight mature miRNAs were downregulated in subjects from HAM compared with ASP. Three of these miRs (hsa-miR-185-5p, 32-5p, and 192-5p) have the potential to be used as biomarkers for predicting the pathogenesis of HAM/TSP. The seven most deregulated miRs target genes have been associated with a variety of biological processes and molecular functions. The reactome pathways relevant to our findings provide a rich source of data and offer the opportunity to better understand sRNA regulation and function in HTLV-1 pathophysiology. To the best of our knowledge, this study is the first to demonstrate evaluates sRNAs in HTLV-1 patients with HAM/TSP.https://www.tandfonline.com/doi/10.1080/21505594.2023.2230015Small RNAHTLV-1HAM/TSPmassive parallel sequencing |
| spellingShingle | Daniela Raguer Valadão de Souza Rodrigo Pessôa Youko Nukui Juliana Pereira Rosa Nascimento Marcusso Augusto César Penalva de Oliveira Jorge Casseb Alberto José da Silva Duarte Patricia Bianca Clissa Sabri Saeed Sanabani Identification of miRnas with possible prognostic roles for HAM/TSP Virulence Small RNA HTLV-1 HAM/TSP massive parallel sequencing |
| title | Identification of miRnas with possible prognostic roles for HAM/TSP |
| title_full | Identification of miRnas with possible prognostic roles for HAM/TSP |
| title_fullStr | Identification of miRnas with possible prognostic roles for HAM/TSP |
| title_full_unstemmed | Identification of miRnas with possible prognostic roles for HAM/TSP |
| title_short | Identification of miRnas with possible prognostic roles for HAM/TSP |
| title_sort | identification of mirnas with possible prognostic roles for ham tsp |
| topic | Small RNA HTLV-1 HAM/TSP massive parallel sequencing |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2023.2230015 |
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