Oral Uptake of Chlamydia psittaci by Ducklings Results in Systemic Dissemination.
Enteric infections caused by Chlamydia (C.) psittaci are frequent in ducks, but mostly remain subclinical under field conditions. To emulate natural infection, we investigated the pathogenic potential of a C. psittaci field strain in orally inoculated 4-day-old ducklings. Three different challenge d...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4864072?pdf=render |
| _version_ | 1818947319240851456 |
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| author | Simon Thierry Fabien Vorimore Christelle Rossignol Sabine Scharf Konrad Sachse Patricia Berthon Benoit Durand Isabelle Virlogeux-Payant Nicole Borel Karine Laroucau |
| author_facet | Simon Thierry Fabien Vorimore Christelle Rossignol Sabine Scharf Konrad Sachse Patricia Berthon Benoit Durand Isabelle Virlogeux-Payant Nicole Borel Karine Laroucau |
| author_sort | Simon Thierry |
| collection | DOAJ |
| description | Enteric infections caused by Chlamydia (C.) psittaci are frequent in ducks, but mostly remain subclinical under field conditions. To emulate natural infection, we investigated the pathogenic potential of a C. psittaci field strain in orally inoculated 4-day-old ducklings. Three different challenge doses were tested and seven contact animals were also mock-inoculated with buffer in each group. Over the course of ten days, the birds were monitored for clinical symptoms and chlamydial dissemination before final examination of tissues using histopathology and immunohistochemistry. While the challenge strain disseminated systemically to all internal organs, mild signs of diarrhea were confined to ducklings inoculated with the highest dose (4.3 x 108 IFU/mL, Group 1). No other clinical symptoms or histopathological lesions were seen. The chlamydial load in internal organs as measured by PCR depended on the challenge dose and was unevenly distributed, i.e. high loads in spleen, liver, and distal small and large intestinal tract (ileum, cecum and rectum) vs. ten times lower values in lungs and proximal small intestinal tract (duodenum and jejunum). Notably, the C. psittaci infection of contact birds became evident on day 10 post-infection, with bacterial loads comparable to those of experimentally-infected animals, thus suggesting rapid bird-to-bird transmission of the challenge strain. |
| first_indexed | 2024-12-20T08:29:01Z |
| format | Article |
| id | doaj.art-b92dd9e66ec1405dbc266ec5ee666c0a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T08:29:01Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b92dd9e66ec1405dbc266ec5ee666c0a2022-12-21T19:46:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01115e015486010.1371/journal.pone.0154860Oral Uptake of Chlamydia psittaci by Ducklings Results in Systemic Dissemination.Simon ThierryFabien VorimoreChristelle RossignolSabine ScharfKonrad SachsePatricia BerthonBenoit DurandIsabelle Virlogeux-PayantNicole BorelKarine LaroucauEnteric infections caused by Chlamydia (C.) psittaci are frequent in ducks, but mostly remain subclinical under field conditions. To emulate natural infection, we investigated the pathogenic potential of a C. psittaci field strain in orally inoculated 4-day-old ducklings. Three different challenge doses were tested and seven contact animals were also mock-inoculated with buffer in each group. Over the course of ten days, the birds were monitored for clinical symptoms and chlamydial dissemination before final examination of tissues using histopathology and immunohistochemistry. While the challenge strain disseminated systemically to all internal organs, mild signs of diarrhea were confined to ducklings inoculated with the highest dose (4.3 x 108 IFU/mL, Group 1). No other clinical symptoms or histopathological lesions were seen. The chlamydial load in internal organs as measured by PCR depended on the challenge dose and was unevenly distributed, i.e. high loads in spleen, liver, and distal small and large intestinal tract (ileum, cecum and rectum) vs. ten times lower values in lungs and proximal small intestinal tract (duodenum and jejunum). Notably, the C. psittaci infection of contact birds became evident on day 10 post-infection, with bacterial loads comparable to those of experimentally-infected animals, thus suggesting rapid bird-to-bird transmission of the challenge strain.http://europepmc.org/articles/PMC4864072?pdf=render |
| spellingShingle | Simon Thierry Fabien Vorimore Christelle Rossignol Sabine Scharf Konrad Sachse Patricia Berthon Benoit Durand Isabelle Virlogeux-Payant Nicole Borel Karine Laroucau Oral Uptake of Chlamydia psittaci by Ducklings Results in Systemic Dissemination. PLoS ONE |
| title | Oral Uptake of Chlamydia psittaci by Ducklings Results in Systemic Dissemination. |
| title_full | Oral Uptake of Chlamydia psittaci by Ducklings Results in Systemic Dissemination. |
| title_fullStr | Oral Uptake of Chlamydia psittaci by Ducklings Results in Systemic Dissemination. |
| title_full_unstemmed | Oral Uptake of Chlamydia psittaci by Ducklings Results in Systemic Dissemination. |
| title_short | Oral Uptake of Chlamydia psittaci by Ducklings Results in Systemic Dissemination. |
| title_sort | oral uptake of chlamydia psittaci by ducklings results in systemic dissemination |
| url | http://europepmc.org/articles/PMC4864072?pdf=render |
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