Analysis of Same Selected Immunomodulatory Properties of Chorionic Mesenchymal Stem Cells
Mesenchymal stem cells (MSCs) represent a population of adherent cells that can be isolated from multiple adult tissues. MSCs have immunomodulatory capacity and the ability to differentiate into many cell lines. Research study examines the immunomodulatory properties of MSCs isolated from chorion (C...
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MDPI AG
2020-12-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/24/9040 |
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| author | Darina Bačenková Marianna Trebuňová Lukáš Zachar Radovan Hudák Gabriela Ižaríková Katarína Šurínová Jozef Živčák |
| author_facet | Darina Bačenková Marianna Trebuňová Lukáš Zachar Radovan Hudák Gabriela Ižaríková Katarína Šurínová Jozef Živčák |
| author_sort | Darina Bačenková |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) represent a population of adherent cells that can be isolated from multiple adult tissues. MSCs have immunomodulatory capacity and the ability to differentiate into many cell lines. Research study examines the immunomodulatory properties of MSCs isolated from chorion (CMSCs). Following the stimulation process, it was found that MSCs are capable of immunomodulatory action via the release of bioactive molecules as well as through direct contact with the immune cells. Immunomodulatory potential of the CMSCs was analyzed by modifying proliferative capacity of mitogen-activated lymphocytes. CMSCs and lymphocytes were tested in cell-to-cell contact. Lymphocytes were stained with carboxyfluorescein diacetate succinimidyl ester. Inhibition of the proliferation of activated lymphocytes was observed. Following the co-cultivation, the expression of markers involved in the immune response modulation was assessed. Afterwards, an increase in CMSCs expression of IL-10 was detected. Following the co-cultivation with activated lymphocyte, adhesion molecules CD54 and CD44 in the CMSCs increased. An increase of CD54 expression was observed. The properties of CMSCs, adherence and differentiation ability, were confirmed. The phenotype of CMSCs CD105+, CD90+, CD73+, CD44+, CD29+, CD45−, CD34−, CD54+ was characterized. It was demonstrated that chorion-derived MSCs have important immunomodulatory effects. |
| first_indexed | 2024-03-10T13:59:23Z |
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| id | doaj.art-b93282a2c6694a03927b5aa7746fda88 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T13:59:23Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-b93282a2c6694a03927b5aa7746fda882023-11-21T01:19:47ZengMDPI AGApplied Sciences2076-34172020-12-011024904010.3390/app10249040Analysis of Same Selected Immunomodulatory Properties of Chorionic Mesenchymal Stem CellsDarina Bačenková0Marianna Trebuňová1Lukáš Zachar2Radovan Hudák3Gabriela Ižaríková4Katarína Šurínová5Jozef Živčák6Department of Biomedical Engineering and Measurement, Faculty of Mechanical Engineering, Technical University of Košice, Letná 9, 042 00 Košice, SlovakiaDepartment of Biomedical Engineering and Measurement, Faculty of Mechanical Engineering, Technical University of Košice, Letná 9, 042 00 Košice, SlovakiaDepartment of Laboratory Medicine, University Hospital of Louis Pasteur, Trieda SNP 1, 041 66 Košice, SlovakiaDepartment of Biomedical Engineering and Measurement, Faculty of Mechanical Engineering, Technical University of Košice, Letná 9, 042 00 Košice, SlovakiaDepartment of Applied Mathematics and Informatics, Faculty of Mechanical Engineering, Technical University of Košice, Letná 9, 042 00 Košice, SlovakiaDepartment of Biomedical Engineering and Measurement, Faculty of Mechanical Engineering, Technical University of Košice, Letná 9, 042 00 Košice, SlovakiaDepartment of Biomedical Engineering and Measurement, Faculty of Mechanical Engineering, Technical University of Košice, Letná 9, 042 00 Košice, SlovakiaMesenchymal stem cells (MSCs) represent a population of adherent cells that can be isolated from multiple adult tissues. MSCs have immunomodulatory capacity and the ability to differentiate into many cell lines. Research study examines the immunomodulatory properties of MSCs isolated from chorion (CMSCs). Following the stimulation process, it was found that MSCs are capable of immunomodulatory action via the release of bioactive molecules as well as through direct contact with the immune cells. Immunomodulatory potential of the CMSCs was analyzed by modifying proliferative capacity of mitogen-activated lymphocytes. CMSCs and lymphocytes were tested in cell-to-cell contact. Lymphocytes were stained with carboxyfluorescein diacetate succinimidyl ester. Inhibition of the proliferation of activated lymphocytes was observed. Following the co-cultivation, the expression of markers involved in the immune response modulation was assessed. Afterwards, an increase in CMSCs expression of IL-10 was detected. Following the co-cultivation with activated lymphocyte, adhesion molecules CD54 and CD44 in the CMSCs increased. An increase of CD54 expression was observed. The properties of CMSCs, adherence and differentiation ability, were confirmed. The phenotype of CMSCs CD105+, CD90+, CD73+, CD44+, CD29+, CD45−, CD34−, CD54+ was characterized. It was demonstrated that chorion-derived MSCs have important immunomodulatory effects.https://www.mdpi.com/2076-3417/10/24/9040chorionic mesenchymal stem cellsimmunomodulationlymphocytes |
| spellingShingle | Darina Bačenková Marianna Trebuňová Lukáš Zachar Radovan Hudák Gabriela Ižaríková Katarína Šurínová Jozef Živčák Analysis of Same Selected Immunomodulatory Properties of Chorionic Mesenchymal Stem Cells Applied Sciences chorionic mesenchymal stem cells immunomodulation lymphocytes |
| title | Analysis of Same Selected Immunomodulatory Properties of Chorionic Mesenchymal Stem Cells |
| title_full | Analysis of Same Selected Immunomodulatory Properties of Chorionic Mesenchymal Stem Cells |
| title_fullStr | Analysis of Same Selected Immunomodulatory Properties of Chorionic Mesenchymal Stem Cells |
| title_full_unstemmed | Analysis of Same Selected Immunomodulatory Properties of Chorionic Mesenchymal Stem Cells |
| title_short | Analysis of Same Selected Immunomodulatory Properties of Chorionic Mesenchymal Stem Cells |
| title_sort | analysis of same selected immunomodulatory properties of chorionic mesenchymal stem cells |
| topic | chorionic mesenchymal stem cells immunomodulation lymphocytes |
| url | https://www.mdpi.com/2076-3417/10/24/9040 |
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