Physiological and Proteomic Analyses of Molybdenum- and Ethylene-Responsive Mechanisms in Rubber Latex
Molybdenum (Mo) is an essential micronutrient in many plants. In the rubber tree Hevea brasiliensis, Mo application can reduce the shrinkage of the tapping line, decrease tapping panel dryness, and finally increase rubber latex yield. After combined Mo with ethylene (Eth), these effects become more...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-05-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2018.00621/full |
| _version_ | 1818700662244900864 |
|---|---|
| author | Le Gao Le Gao Yong Sun Yong Sun Min Wu Dan Wang Jiashao Wei Bingsun Wu Guihua Wang Wenguan Wu Xiang Jin Xiang Jin Xuchu Wang Xuchu Wang Peng He Peng He |
| author_facet | Le Gao Le Gao Yong Sun Yong Sun Min Wu Dan Wang Jiashao Wei Bingsun Wu Guihua Wang Wenguan Wu Xiang Jin Xiang Jin Xuchu Wang Xuchu Wang Peng He Peng He |
| author_sort | Le Gao |
| collection | DOAJ |
| description | Molybdenum (Mo) is an essential micronutrient in many plants. In the rubber tree Hevea brasiliensis, Mo application can reduce the shrinkage of the tapping line, decrease tapping panel dryness, and finally increase rubber latex yield. After combined Mo with ethylene (Eth), these effects become more obvious. However, the molecular mechanism remains unclear. Here, we compared the changed patterns of physiological parameters and protein accumulation in rubber latex after treated with Mo and/or Eth. Our results demonstrated that both Eth and Mo can improve the contents of thiol, sucrose, and dry yield in rubber latex. However, lutoid bursting is significantly inhibited by Mo. Comparative proteomics identified 169 differentially expressed proteins, including 114 unique proteins, which are mainly involved in posttranslational modification, carbohydrate metabolism, and energy production. The abundances of several proteins involved in rubber particle aggregation are decreased upon Mo stimulation, while many enzymes related to natural rubber biosynthesis are increased. Comparison of the accumulation patterns of 25 proteins revealed that a large portion of proteins have different changed patterns with their gene expression levels. Activity assays of six enzymes revealed that Mo stimulation can increase latex yield by improving the activity of some Mo-responsive enzymes. These results not only deepen our understanding of the rubber latex proteome but also provide new insights into the molecular mechanism of Mo-stimulated rubber latex yield. |
| first_indexed | 2024-12-17T15:08:30Z |
| format | Article |
| id | doaj.art-b9354da7fb5046b0a6ad2fcf3d4d8e53 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-17T15:08:30Z |
| publishDate | 2018-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-b9354da7fb5046b0a6ad2fcf3d4d8e532022-12-21T21:43:44ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-05-01910.3389/fpls.2018.00621355917Physiological and Proteomic Analyses of Molybdenum- and Ethylene-Responsive Mechanisms in Rubber LatexLe Gao0Le Gao1Yong Sun2Yong Sun3Min Wu4Dan Wang5Jiashao Wei6Bingsun Wu7Guihua Wang8Wenguan Wu9Xiang Jin10Xiang Jin11Xuchu Wang12Xuchu Wang13Peng He14Peng He15Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaCollege of Life Sciences, Key Laboratory for Ecology of Tropical Islands, Ministry of Education, Hainan Normal University, Haikou, ChinaRubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaCollege of Life Sciences, Key Laboratory for Ecology of Tropical Islands, Ministry of Education, Hainan Normal University, Haikou, ChinaRubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaInstitute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaRubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaRubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaRubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaRubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaCollege of Life Sciences, Key Laboratory for Ecology of Tropical Islands, Ministry of Education, Hainan Normal University, Haikou, ChinaInstitute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaCollege of Life Sciences, Key Laboratory for Ecology of Tropical Islands, Ministry of Education, Hainan Normal University, Haikou, ChinaInstitute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaRubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaCollege of Life Sciences, Key Laboratory for Ecology of Tropical Islands, Ministry of Education, Hainan Normal University, Haikou, ChinaMolybdenum (Mo) is an essential micronutrient in many plants. In the rubber tree Hevea brasiliensis, Mo application can reduce the shrinkage of the tapping line, decrease tapping panel dryness, and finally increase rubber latex yield. After combined Mo with ethylene (Eth), these effects become more obvious. However, the molecular mechanism remains unclear. Here, we compared the changed patterns of physiological parameters and protein accumulation in rubber latex after treated with Mo and/or Eth. Our results demonstrated that both Eth and Mo can improve the contents of thiol, sucrose, and dry yield in rubber latex. However, lutoid bursting is significantly inhibited by Mo. Comparative proteomics identified 169 differentially expressed proteins, including 114 unique proteins, which are mainly involved in posttranslational modification, carbohydrate metabolism, and energy production. The abundances of several proteins involved in rubber particle aggregation are decreased upon Mo stimulation, while many enzymes related to natural rubber biosynthesis are increased. Comparison of the accumulation patterns of 25 proteins revealed that a large portion of proteins have different changed patterns with their gene expression levels. Activity assays of six enzymes revealed that Mo stimulation can increase latex yield by improving the activity of some Mo-responsive enzymes. These results not only deepen our understanding of the rubber latex proteome but also provide new insights into the molecular mechanism of Mo-stimulated rubber latex yield.http://journal.frontiersin.org/article/10.3389/fpls.2018.00621/fullcomparative proteomicsethyleneHevea brasiliensismolybdenum stimulationnatural rubber biosynthesisrubber latex |
| spellingShingle | Le Gao Le Gao Yong Sun Yong Sun Min Wu Dan Wang Jiashao Wei Bingsun Wu Guihua Wang Wenguan Wu Xiang Jin Xiang Jin Xuchu Wang Xuchu Wang Peng He Peng He Physiological and Proteomic Analyses of Molybdenum- and Ethylene-Responsive Mechanisms in Rubber Latex Frontiers in Plant Science comparative proteomics ethylene Hevea brasiliensis molybdenum stimulation natural rubber biosynthesis rubber latex |
| title | Physiological and Proteomic Analyses of Molybdenum- and Ethylene-Responsive Mechanisms in Rubber Latex |
| title_full | Physiological and Proteomic Analyses of Molybdenum- and Ethylene-Responsive Mechanisms in Rubber Latex |
| title_fullStr | Physiological and Proteomic Analyses of Molybdenum- and Ethylene-Responsive Mechanisms in Rubber Latex |
| title_full_unstemmed | Physiological and Proteomic Analyses of Molybdenum- and Ethylene-Responsive Mechanisms in Rubber Latex |
| title_short | Physiological and Proteomic Analyses of Molybdenum- and Ethylene-Responsive Mechanisms in Rubber Latex |
| title_sort | physiological and proteomic analyses of molybdenum and ethylene responsive mechanisms in rubber latex |
| topic | comparative proteomics ethylene Hevea brasiliensis molybdenum stimulation natural rubber biosynthesis rubber latex |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2018.00621/full |
| work_keys_str_mv | AT legao physiologicalandproteomicanalysesofmolybdenumandethyleneresponsivemechanismsinrubberlatex AT legao physiologicalandproteomicanalysesofmolybdenumandethyleneresponsivemechanismsinrubberlatex AT yongsun physiologicalandproteomicanalysesofmolybdenumandethyleneresponsivemechanismsinrubberlatex AT yongsun physiologicalandproteomicanalysesofmolybdenumandethyleneresponsivemechanismsinrubberlatex AT minwu physiologicalandproteomicanalysesofmolybdenumandethyleneresponsivemechanismsinrubberlatex AT danwang physiologicalandproteomicanalysesofmolybdenumandethyleneresponsivemechanismsinrubberlatex AT jiashaowei physiologicalandproteomicanalysesofmolybdenumandethyleneresponsivemechanismsinrubberlatex AT bingsunwu physiologicalandproteomicanalysesofmolybdenumandethyleneresponsivemechanismsinrubberlatex AT guihuawang physiologicalandproteomicanalysesofmolybdenumandethyleneresponsivemechanismsinrubberlatex AT wenguanwu physiologicalandproteomicanalysesofmolybdenumandethyleneresponsivemechanismsinrubberlatex AT xiangjin physiologicalandproteomicanalysesofmolybdenumandethyleneresponsivemechanismsinrubberlatex AT xiangjin physiologicalandproteomicanalysesofmolybdenumandethyleneresponsivemechanismsinrubberlatex AT xuchuwang physiologicalandproteomicanalysesofmolybdenumandethyleneresponsivemechanismsinrubberlatex AT xuchuwang physiologicalandproteomicanalysesofmolybdenumandethyleneresponsivemechanismsinrubberlatex AT penghe physiologicalandproteomicanalysesofmolybdenumandethyleneresponsivemechanismsinrubberlatex AT penghe physiologicalandproteomicanalysesofmolybdenumandethyleneresponsivemechanismsinrubberlatex |