Characterization of a novel 8R,11S-linoleate diol synthase from Penicillium chrysogenum by identification of its enzymatic products[S]
To identify novel fatty acid diol synthases, putative candidate sequences from Penicillium species were analyzed, and hydroxy fatty acid production by crude Penicillium enzyme extracts was assessed. Penicillium chrysogenum was found to produce an unknown dihydroxy fatty acid, a candidate gene implic...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-02-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520354572 |
| _version_ | 1818957116664184832 |
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| author | Kyung-Chul Shin Min-Ju Seo Deok-Kun Oh |
| author_facet | Kyung-Chul Shin Min-Ju Seo Deok-Kun Oh |
| author_sort | Kyung-Chul Shin |
| collection | DOAJ |
| description | To identify novel fatty acid diol synthases, putative candidate sequences from Penicillium species were analyzed, and hydroxy fatty acid production by crude Penicillium enzyme extracts was assessed. Penicillium chrysogenum was found to produce an unknown dihydroxy fatty acid, a candidate gene implicated in this production was cloned and expressed, and the expressed enzyme was purified. The product obtained by the reaction of the purified enzyme with linoleic acid was identified as 8R,11S-dihydroxy-9,12(Z,Z)-octadecadienoic acid (8R,11S-DiHODE). The catalytic efficiency of this enzyme toward linoleic acid was the highest among the unsaturated fatty acids tested, indicating that this enzyme was a novel 8R,11S-linoleate diol synthase (8R,11S-LDS). A sexual stage in the life cycle of P. chrysogenum has recently been discovered, and 8R,11S-DiHODE produced by 8R,11S-LDS may constitute a precocious sexual inducer factor, responsible for regulating the sexual and asexual cycles of this fungus. |
| first_indexed | 2024-12-20T11:04:44Z |
| format | Article |
| id | doaj.art-b93cd405cc0d4706acf6ed8e8461c6ff |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T11:04:44Z |
| publishDate | 2016-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-b93cd405cc0d4706acf6ed8e8461c6ff2022-12-21T19:42:53ZengElsevierJournal of Lipid Research0022-22752016-02-01572207218Characterization of a novel 8R,11S-linoleate diol synthase from Penicillium chrysogenum by identification of its enzymatic products[S]Kyung-Chul Shin0Min-Ju Seo1Deok-Kun Oh2Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of KoreaTo whom correspondence should be addressed; To whom correspondence should be addressed; Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of KoreaTo identify novel fatty acid diol synthases, putative candidate sequences from Penicillium species were analyzed, and hydroxy fatty acid production by crude Penicillium enzyme extracts was assessed. Penicillium chrysogenum was found to produce an unknown dihydroxy fatty acid, a candidate gene implicated in this production was cloned and expressed, and the expressed enzyme was purified. The product obtained by the reaction of the purified enzyme with linoleic acid was identified as 8R,11S-dihydroxy-9,12(Z,Z)-octadecadienoic acid (8R,11S-DiHODE). The catalytic efficiency of this enzyme toward linoleic acid was the highest among the unsaturated fatty acids tested, indicating that this enzyme was a novel 8R,11S-linoleate diol synthase (8R,11S-LDS). A sexual stage in the life cycle of P. chrysogenum has recently been discovered, and 8R,11S-DiHODE produced by 8R,11S-LDS may constitute a precocious sexual inducer factor, responsible for regulating the sexual and asexual cycles of this fungus.http://www.sciencedirect.com/science/article/pii/S00222275203545728R,11S-dihydroxy-9,12(Z,Z)-octadecadienoic acidhydroxy fatty acidprecocious sexual inducer factor |
| spellingShingle | Kyung-Chul Shin Min-Ju Seo Deok-Kun Oh Characterization of a novel 8R,11S-linoleate diol synthase from Penicillium chrysogenum by identification of its enzymatic products[S] Journal of Lipid Research 8R,11S-dihydroxy-9,12(Z,Z)-octadecadienoic acid hydroxy fatty acid precocious sexual inducer factor |
| title | Characterization of a novel 8R,11S-linoleate diol synthase from Penicillium chrysogenum by identification of its enzymatic products[S] |
| title_full | Characterization of a novel 8R,11S-linoleate diol synthase from Penicillium chrysogenum by identification of its enzymatic products[S] |
| title_fullStr | Characterization of a novel 8R,11S-linoleate diol synthase from Penicillium chrysogenum by identification of its enzymatic products[S] |
| title_full_unstemmed | Characterization of a novel 8R,11S-linoleate diol synthase from Penicillium chrysogenum by identification of its enzymatic products[S] |
| title_short | Characterization of a novel 8R,11S-linoleate diol synthase from Penicillium chrysogenum by identification of its enzymatic products[S] |
| title_sort | characterization of a novel 8r 11s linoleate diol synthase from penicillium chrysogenum by identification of its enzymatic products s |
| topic | 8R,11S-dihydroxy-9,12(Z,Z)-octadecadienoic acid hydroxy fatty acid precocious sexual inducer factor |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520354572 |
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