A Disintegrin and Metalloprotease 10 Expressions Modulate Potential Metastatic and Thrombus Formation in Pancreatic Carcinoma
Background: Clinical investigations repurposing a disintegrin and metalloproteases 10 (ADAM10) as metastatic and thrombus marker have achieved encouraging results, but the mechanism behind this association remains unclear. Methods: This study was carried out in NingXia and Wuhan, China from 2017...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2022-08-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/27670 |
| _version_ | 1798037271210885120 |
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| author | Miao Yu Quan Chen Qin Li Yunfei Teng Lin Xiao Guang Yang Chenxi Ouyang Ahmed Mohammed Elamin Abdalla |
| author_facet | Miao Yu Quan Chen Qin Li Yunfei Teng Lin Xiao Guang Yang Chenxi Ouyang Ahmed Mohammed Elamin Abdalla |
| author_sort | Miao Yu |
| collection | DOAJ |
| description |
Background: Clinical investigations repurposing a disintegrin and metalloproteases 10 (ADAM10) as metastatic and thrombus marker have achieved encouraging results, but the mechanism behind this association remains unclear.
Methods: This study was carried out in NingXia and Wuhan, China from 2017 to 2021. The effects of ADAM10 expression on the metastatic and thrombus-associated genes: tissue factor (TF), P-selectin glycoprotein ligand-1 (PSGL-1), cathepsin G (CTSG) and mucin 1 (MUC1) were examined by immunofluorescence, qRT-PCR and Western blotting analysis. Metastatic and thrombotic behaviors were evaluated using NODSCID mouse model.
Results: The ADAM10 expression controlled the migration and invasion of pancreatic carcinoma cell-1(PANC-1), and significantly regulated the metastatic and thrombus-associated genes (P＜0.05). ADAM10 and MUC1 were regulated and aberrantly expressed by a dependent mechanism. Moreover, ADAM10 expression induced the progression of adenocarcinoma cells and thrombus formation in vivo.
Conclusion: Regulation of ADAM10 expression in cancer cells might effectively pave the way for a more potent anti-metastatic and anti-thrombotic approach and could regulate the invasion and migration of cancer cells.
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| first_indexed | 2024-04-11T21:24:19Z |
| format | Article |
| id | doaj.art-b943b591de9f41b2a50df0c23209acad |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-04-11T21:24:19Z |
| publishDate | 2022-08-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-b943b591de9f41b2a50df0c23209acad2022-12-22T04:02:29ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932022-08-0151810.18502/ijph.v51i8.10262A Disintegrin and Metalloprotease 10 Expressions Modulate Potential Metastatic and Thrombus Formation in Pancreatic CarcinomaMiao Yu0Quan Chen1Qin Li2Yunfei Teng3Lin Xiao4Guang Yang5Chenxi Ouyang6Ahmed Mohammed Elamin Abdalla7Department of Vascular Surgery, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, ChinaDepartment of Vascular Surgery, Gansu Provincial Hospital, Lanzhou 730000, ChinaDepartment of Vascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wu-han 430022, ChinaDepartment of Vascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wu-han 430022, ChinaDepartment of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaDepartment of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaDepartment of Vascular Surgery, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China1. Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China 2. Department of Biochemistry, College of Applied Science, University of Bahri, Khartoum 1660/11111, Sudan Background: Clinical investigations repurposing a disintegrin and metalloproteases 10 (ADAM10) as metastatic and thrombus marker have achieved encouraging results, but the mechanism behind this association remains unclear. Methods: This study was carried out in NingXia and Wuhan, China from 2017 to 2021. The effects of ADAM10 expression on the metastatic and thrombus-associated genes: tissue factor (TF), P-selectin glycoprotein ligand-1 (PSGL-1), cathepsin G (CTSG) and mucin 1 (MUC1) were examined by immunofluorescence, qRT-PCR and Western blotting analysis. Metastatic and thrombotic behaviors were evaluated using NODSCID mouse model. Results: The ADAM10 expression controlled the migration and invasion of pancreatic carcinoma cell-1(PANC-1), and significantly regulated the metastatic and thrombus-associated genes (P＜0.05). ADAM10 and MUC1 were regulated and aberrantly expressed by a dependent mechanism. Moreover, ADAM10 expression induced the progression of adenocarcinoma cells and thrombus formation in vivo. Conclusion: Regulation of ADAM10 expression in cancer cells might effectively pave the way for a more potent anti-metastatic and anti-thrombotic approach and could regulate the invasion and migration of cancer cells. https://ijph.tums.ac.ir/index.php/ijph/article/view/27670ADAM10 proteinMetastasisThrombosis |
| spellingShingle | Miao Yu Quan Chen Qin Li Yunfei Teng Lin Xiao Guang Yang Chenxi Ouyang Ahmed Mohammed Elamin Abdalla A Disintegrin and Metalloprotease 10 Expressions Modulate Potential Metastatic and Thrombus Formation in Pancreatic Carcinoma Iranian Journal of Public Health ADAM10 protein Metastasis Thrombosis |
| title | A Disintegrin and Metalloprotease 10 Expressions Modulate Potential Metastatic and Thrombus Formation in Pancreatic Carcinoma |
| title_full | A Disintegrin and Metalloprotease 10 Expressions Modulate Potential Metastatic and Thrombus Formation in Pancreatic Carcinoma |
| title_fullStr | A Disintegrin and Metalloprotease 10 Expressions Modulate Potential Metastatic and Thrombus Formation in Pancreatic Carcinoma |
| title_full_unstemmed | A Disintegrin and Metalloprotease 10 Expressions Modulate Potential Metastatic and Thrombus Formation in Pancreatic Carcinoma |
| title_short | A Disintegrin and Metalloprotease 10 Expressions Modulate Potential Metastatic and Thrombus Formation in Pancreatic Carcinoma |
| title_sort | disintegrin and metalloprotease 10 expressions modulate potential metastatic and thrombus formation in pancreatic carcinoma |
| topic | ADAM10 protein Metastasis Thrombosis |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/27670 |
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