Development of Optimized Vitrification Procedures Using Closed Carrier System to Improve the Survival and Developmental Competence of Vitrified Mouse Oocytes
The open carrier system (OC) is used for vitrification due to its high efficiency in preserving female fertility, but concerns remain that it bears possible risks of cross-contamination. Closed carrier systems (CC) could be an alternative to the OC to increase safety. However, the viability and deve...
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MDPI AG
2021-07-01
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| Online Access: | https://www.mdpi.com/2073-4409/10/7/1670 |
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| author | Jae Kyun Park Ju Hee Lee Eun A Park Hyunjung J. Lim Sang Woo Lyu Woo Sik Lee Jayeon Kim Haengseok Song |
| author_facet | Jae Kyun Park Ju Hee Lee Eun A Park Hyunjung J. Lim Sang Woo Lyu Woo Sik Lee Jayeon Kim Haengseok Song |
| author_sort | Jae Kyun Park |
| collection | DOAJ |
| description | The open carrier system (OC) is used for vitrification due to its high efficiency in preserving female fertility, but concerns remain that it bears possible risks of cross-contamination. Closed carrier systems (CC) could be an alternative to the OC to increase safety. However, the viability and developmental competence of vitrified/warmed (VW) oocytes using the CC were significantly lower than with OC. We aimed to improve the efficiency of the CC. Metaphase II oocytes were collected from mice after superovulation and subjected to in vitro fertilization after vitrification/warming. Increasing the cooling/warming rate and exposure time to cryoprotectants as key parameters for the CC effectively improved the survival rate and developmental competence of VW oocytes. When all the conditions that improved the outcomes were applied to the conventional CC, hereafter named the modified vitrification/warming procedure using CC (mVW-CC), the viability and developmental competence of VW oocytes were significantly improved as compared to those of VW oocytes in the CC. Furthermore, mVW-CC increased the spindle normality of VW oocytes, as well as the cell number of blastocysts developed from VW oocytes. Collectively, our mVW-CC optimized for mouse oocytes can be utilized for humans without concerns regarding possible cross-contamination during vitrification in the future. |
| first_indexed | 2024-03-10T09:43:25Z |
| format | Article |
| id | doaj.art-b947bc9217e5484596731e4d9a139af5 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T09:43:25Z |
| publishDate | 2021-07-01 |
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| series | Cells |
| spelling | doaj.art-b947bc9217e5484596731e4d9a139af52023-11-22T03:28:06ZengMDPI AGCells2073-44092021-07-01107167010.3390/cells10071670Development of Optimized Vitrification Procedures Using Closed Carrier System to Improve the Survival and Developmental Competence of Vitrified Mouse OocytesJae Kyun Park0Ju Hee Lee1Eun A Park2Hyunjung J. Lim3Sang Woo Lyu4Woo Sik Lee5Jayeon Kim6Haengseok Song7Department of Biomedical Sciences, CHA University, Seongnam 13488, KoreaDepartment of Biomedical Sciences, CHA University, Seongnam 13488, KoreaCHA Fertility Center Seoul Station, CHA University, Seoul 04637, KoreaDepartment of Veterinary Medicine, School of Veterinary Medicine, Konkuk University, Seoul 05029, KoreaCHA Fertility Center Gangnam, CHA University, Seoul 06125, KoreaCHA Fertility Center Gangnam, CHA University, Seoul 06125, KoreaCHA Fertility Center Seoul Station, CHA University, Seoul 04637, KoreaDepartment of Biomedical Sciences, CHA University, Seongnam 13488, KoreaThe open carrier system (OC) is used for vitrification due to its high efficiency in preserving female fertility, but concerns remain that it bears possible risks of cross-contamination. Closed carrier systems (CC) could be an alternative to the OC to increase safety. However, the viability and developmental competence of vitrified/warmed (VW) oocytes using the CC were significantly lower than with OC. We aimed to improve the efficiency of the CC. Metaphase II oocytes were collected from mice after superovulation and subjected to in vitro fertilization after vitrification/warming. Increasing the cooling/warming rate and exposure time to cryoprotectants as key parameters for the CC effectively improved the survival rate and developmental competence of VW oocytes. When all the conditions that improved the outcomes were applied to the conventional CC, hereafter named the modified vitrification/warming procedure using CC (mVW-CC), the viability and developmental competence of VW oocytes were significantly improved as compared to those of VW oocytes in the CC. Furthermore, mVW-CC increased the spindle normality of VW oocytes, as well as the cell number of blastocysts developed from VW oocytes. Collectively, our mVW-CC optimized for mouse oocytes can be utilized for humans without concerns regarding possible cross-contamination during vitrification in the future.https://www.mdpi.com/2073-4409/10/7/1670oocyte vitrificationclosed carrier systemscross-contaminationcooling ratewarming rateexposure time to cryoprotectants |
| spellingShingle | Jae Kyun Park Ju Hee Lee Eun A Park Hyunjung J. Lim Sang Woo Lyu Woo Sik Lee Jayeon Kim Haengseok Song Development of Optimized Vitrification Procedures Using Closed Carrier System to Improve the Survival and Developmental Competence of Vitrified Mouse Oocytes Cells oocyte vitrification closed carrier systems cross-contamination cooling rate warming rate exposure time to cryoprotectants |
| title | Development of Optimized Vitrification Procedures Using Closed Carrier System to Improve the Survival and Developmental Competence of Vitrified Mouse Oocytes |
| title_full | Development of Optimized Vitrification Procedures Using Closed Carrier System to Improve the Survival and Developmental Competence of Vitrified Mouse Oocytes |
| title_fullStr | Development of Optimized Vitrification Procedures Using Closed Carrier System to Improve the Survival and Developmental Competence of Vitrified Mouse Oocytes |
| title_full_unstemmed | Development of Optimized Vitrification Procedures Using Closed Carrier System to Improve the Survival and Developmental Competence of Vitrified Mouse Oocytes |
| title_short | Development of Optimized Vitrification Procedures Using Closed Carrier System to Improve the Survival and Developmental Competence of Vitrified Mouse Oocytes |
| title_sort | development of optimized vitrification procedures using closed carrier system to improve the survival and developmental competence of vitrified mouse oocytes |
| topic | oocyte vitrification closed carrier systems cross-contamination cooling rate warming rate exposure time to cryoprotectants |
| url | https://www.mdpi.com/2073-4409/10/7/1670 |
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