Protective effects of lipoic acid-niacin dimers against blue light-induced oxidative damage to retinal pigment epithelium cells
AIM: To evaluate the protective effects of lipoic acid-niacin (N2L) dimers against blue light (BL)-induced oxidative damage to human retinal pigment epithelium (hRPE) cells in vitro. METHODS: hRPE cells were divided into a control group (CG), a BL group, an N2L plus BL irradiation group, an α-lipoi...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2019-08-01
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| Series: | International Journal of Ophthalmology |
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| Online Access: | http://www.ijo.cn/en_publish/2019/8/20190805.pdf |
| _version_ | 1818357913474826240 |
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| author | Xiu-Lan Zou Yong-Zhen Yu Hong-Hua Yu Guan-Feng Wang Rong-Biao Pi Zhe Xu Chu Zhang Wen-Jie Zhou Dan-Dan Li Xuan-Ge Chen Yu-Ping Zou |
| author_facet | Xiu-Lan Zou Yong-Zhen Yu Hong-Hua Yu Guan-Feng Wang Rong-Biao Pi Zhe Xu Chu Zhang Wen-Jie Zhou Dan-Dan Li Xuan-Ge Chen Yu-Ping Zou |
| author_sort | Xiu-Lan Zou |
| collection | DOAJ |
| description | AIM: To evaluate the protective effects of lipoic acid-niacin (N2L) dimers against blue light (BL)-induced oxidative damage to human retinal pigment epithelium (hRPE) cells in vitro.
METHODS: hRPE cells were divided into a control group (CG), a BL group, an N2L plus BL irradiation group, an α-lipoic acid (ALA) plus BL group, an ALA-only group, and an N2L-only group. hRPE cellular viability was detected by performing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) bromide assays, and apoptosis was evaluated by annexin-V-PE/7-AAD staining followed by flow cytometry. Ultrastructural changes in subcellular organelles were observed by transmission electron microscopy. Reactive oxygen species formation was assayed by flow cytometry. The expression levels of the apoptosis-related proteins BCL-2 associated X protein (BAX), B-cell leukmia/lymphoma 2 (BCL-2), and caspase-3 were quantified by Western blot analysis.
RESULTS: BL exposure with a light density of 4±0.5 mW/cm2 exceeding 6h caused hRPE toxicity, whereas treatment with a high dose of N2L (100 mol/L) or ALA (150 mol/L) maintained cell viability at control levels. BL exposure caused vacuole-like degeneration, mitochondrial swelling, and reduced microvillus formation; however, a high dose of N2L or ALA maintained the ultrastructure of hRPE cells and their organelles. High doses of N2L and ALA also protected hRPE cells from BL-induced apoptosis, which was confirmed by Western blot analysis: BCL-2 expression significantly increased, while BAX and caspase-3 expression slightly decreased compared to the CG.
CONCLUSION: High-dose N2L treatment (>100 mol/L) can reduce oxidative damage in degenerating hRPE cells exposed to BL with an efficacy similar to ALA. |
| first_indexed | 2024-12-13T20:20:40Z |
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| id | doaj.art-b94c23d0cdfc43c4b1a228bfe7fe2341 |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-13T20:20:40Z |
| publishDate | 2019-08-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-b94c23d0cdfc43c4b1a228bfe7fe23412022-12-21T23:32:42ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982019-08-011281262127110.18240/ijo.2019.08.05Protective effects of lipoic acid-niacin dimers against blue light-induced oxidative damage to retinal pigment epithelium cellsXiu-Lan Zou0Yong-Zhen Yu1Hong-Hua Yu2Guan-Feng Wang3Rong-Biao Pi4Zhe Xu5Chu Zhang6Wen-Jie Zhou7Dan-Dan Li8Xuan-Ge Chen9Yu-Ping Zou10Department of Ophthalmology, General Hospital of Southern Theatre Command of PLA, Guangzhou 510010, Guangdong Province, ChinaDepartment of Ophthalmology, General Hospital of Southern Theatre Command of PLA, Guangzhou 510010, Guangdong Province, ChinaDepartment of Ophthalmology, Guangdong Provincial People’s Hospital, Guangzhou 510000, Guangdong Province, ChinaDepartment of Ophthalmology, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510000, Guangdong Province, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510000, Guangdong Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital, Guangdong Pharmaceutical University, Guangzhou 510000, Guangdong Province, ChinaDepartment of Ophthalmology, General Hospital of Southern Theatre Command of PLA, Guangzhou 510010, Guangdong Province, ChinaDepartment of Ophthalmology, General Hospital of Southern Theatre Command of PLA, Guangzhou 510010, Guangdong Province, ChinaZhongshan Ophthalmic Centre of Sun Yat-sen University, Guangzhou 510000, Guangdong Province, ChinaDepartment of Ophthalmology, General Hospital of Southern Theatre Command of PLA, Guangzhou 510010, Guangdong Province, ChinaDepartment of Ophthalmology, General Hospital of Southern Theatre Command of PLA, Guangzhou 510010, Guangdong Province, ChinaAIM: To evaluate the protective effects of lipoic acid-niacin (N2L) dimers against blue light (BL)-induced oxidative damage to human retinal pigment epithelium (hRPE) cells in vitro. METHODS: hRPE cells were divided into a control group (CG), a BL group, an N2L plus BL irradiation group, an α-lipoic acid (ALA) plus BL group, an ALA-only group, and an N2L-only group. hRPE cellular viability was detected by performing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) bromide assays, and apoptosis was evaluated by annexin-V-PE/7-AAD staining followed by flow cytometry. Ultrastructural changes in subcellular organelles were observed by transmission electron microscopy. Reactive oxygen species formation was assayed by flow cytometry. The expression levels of the apoptosis-related proteins BCL-2 associated X protein (BAX), B-cell leukmia/lymphoma 2 (BCL-2), and caspase-3 were quantified by Western blot analysis. RESULTS: BL exposure with a light density of 4±0.5 mW/cm2 exceeding 6h caused hRPE toxicity, whereas treatment with a high dose of N2L (100 mol/L) or ALA (150 mol/L) maintained cell viability at control levels. BL exposure caused vacuole-like degeneration, mitochondrial swelling, and reduced microvillus formation; however, a high dose of N2L or ALA maintained the ultrastructure of hRPE cells and their organelles. High doses of N2L and ALA also protected hRPE cells from BL-induced apoptosis, which was confirmed by Western blot analysis: BCL-2 expression significantly increased, while BAX and caspase-3 expression slightly decreased compared to the CG. CONCLUSION: High-dose N2L treatment (>100 mol/L) can reduce oxidative damage in degenerating hRPE cells exposed to BL with an efficacy similar to ALA.http://www.ijo.cn/en_publish/2019/8/20190805.pdflipoic acid-niacin dimersretinal pigment epithelium celllipoic acidoxidative stressreactive oxygen speciesapoptosis |
| spellingShingle | Xiu-Lan Zou Yong-Zhen Yu Hong-Hua Yu Guan-Feng Wang Rong-Biao Pi Zhe Xu Chu Zhang Wen-Jie Zhou Dan-Dan Li Xuan-Ge Chen Yu-Ping Zou Protective effects of lipoic acid-niacin dimers against blue light-induced oxidative damage to retinal pigment epithelium cells International Journal of Ophthalmology lipoic acid-niacin dimers retinal pigment epithelium cell lipoic acid oxidative stress reactive oxygen species apoptosis |
| title | Protective effects of lipoic acid-niacin dimers against blue light-induced oxidative damage to retinal pigment epithelium cells |
| title_full | Protective effects of lipoic acid-niacin dimers against blue light-induced oxidative damage to retinal pigment epithelium cells |
| title_fullStr | Protective effects of lipoic acid-niacin dimers against blue light-induced oxidative damage to retinal pigment epithelium cells |
| title_full_unstemmed | Protective effects of lipoic acid-niacin dimers against blue light-induced oxidative damage to retinal pigment epithelium cells |
| title_short | Protective effects of lipoic acid-niacin dimers against blue light-induced oxidative damage to retinal pigment epithelium cells |
| title_sort | protective effects of lipoic acid niacin dimers against blue light induced oxidative damage to retinal pigment epithelium cells |
| topic | lipoic acid-niacin dimers retinal pigment epithelium cell lipoic acid oxidative stress reactive oxygen species apoptosis |
| url | http://www.ijo.cn/en_publish/2019/8/20190805.pdf |
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