Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extract
ABSTRACT Hydroxydihydrobovolide (HDB) was for the first time isolated from Portulaca oleracea L. and then its cytotoxicity against SH-SYTY cells was studied. Moreover, a rapid and sensitive ultra-high performance liquid chromatographic (UHPLC) method with bergapten as internal standard (IS) was deve...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2017-06-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200615&lng=en&tlng=en |
| _version_ | 1823991765408940032 |
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| author | Liang Xu Xiaojun Tao Yucong Gao Wenjie Zhang Yihan Meng Cuiyu Li Mingyue Jiang Ying Xixiang |
| author_facet | Liang Xu Xiaojun Tao Yucong Gao Wenjie Zhang Yihan Meng Cuiyu Li Mingyue Jiang Ying Xixiang |
| author_sort | Liang Xu |
| collection | DOAJ |
| description | ABSTRACT Hydroxydihydrobovolide (HDB) was for the first time isolated from Portulaca oleracea L. and then its cytotoxicity against SH-SYTY cells was studied. Moreover, a rapid and sensitive ultra-high performance liquid chromatographic (UHPLC) method with bergapten as internal standard (IS) was developed and validated to investigate the pharmacokinetics of HDB in rats after intravenous and oral administrations of extract (POE). The UHPLC analysis was performed on a Diamonsil C18 analytical column, using acetonitrile-water (35:65, v/v) as the mobile phase with UV detection at 220 nm. The calibration curve was linear over the range of 0.2-25 µg/mL in rat plasma. The average extraction recovery was from 90.1 to 98.9%, and the relative standard deviations (RSDs) of the intra- and inter-day precisions were less than 4.7 and 4.1%, respectively. The results showed that 50 µM HDB had significant cytotoxicity on the SH-SY5Y cells, which was rapidly distributed with a Tmax of 11 min after oral administration and presented a low absolute bioavailability, 4.12%. |
| first_indexed | 2024-12-18T15:57:50Z |
| format | Article |
| id | doaj.art-b94c652258ce429b97007f0277e029c9 |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-12-18T15:57:50Z |
| publishDate | 2017-06-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-b94c652258ce429b97007f0277e029c92022-12-21T21:02:24ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902017-06-0153210.1590/s2175-97902017000216093S1984-82502017000200615Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extractLiang XuXiaojun TaoYucong GaoWenjie ZhangYihan MengCuiyu LiMingyue JiangYing XixiangABSTRACT Hydroxydihydrobovolide (HDB) was for the first time isolated from Portulaca oleracea L. and then its cytotoxicity against SH-SYTY cells was studied. Moreover, a rapid and sensitive ultra-high performance liquid chromatographic (UHPLC) method with bergapten as internal standard (IS) was developed and validated to investigate the pharmacokinetics of HDB in rats after intravenous and oral administrations of extract (POE). The UHPLC analysis was performed on a Diamonsil C18 analytical column, using acetonitrile-water (35:65, v/v) as the mobile phase with UV detection at 220 nm. The calibration curve was linear over the range of 0.2-25 µg/mL in rat plasma. The average extraction recovery was from 90.1 to 98.9%, and the relative standard deviations (RSDs) of the intra- and inter-day precisions were less than 4.7 and 4.1%, respectively. The results showed that 50 µM HDB had significant cytotoxicity on the SH-SY5Y cells, which was rapidly distributed with a Tmax of 11 min after oral administration and presented a low absolute bioavailability, 4.12%.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200615&lng=en&tlng=enPortulaca oleracea L./hydroxydihydrobovolidePortulaca oleracea L./hydroxydihydrobovolide/cytotoxicityPortulaca oleracea L./hydroxydihydrobovolide/pharmacokineticsHydroxydihydrobovolide/Ultra-high performance liquid chromatography |
| spellingShingle | Liang Xu Xiaojun Tao Yucong Gao Wenjie Zhang Yihan Meng Cuiyu Li Mingyue Jiang Ying Xixiang Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extract Brazilian Journal of Pharmaceutical Sciences Portulaca oleracea L./hydroxydihydrobovolide Portulaca oleracea L./hydroxydihydrobovolide/cytotoxicity Portulaca oleracea L./hydroxydihydrobovolide/pharmacokinetics Hydroxydihydrobovolide/Ultra-high performance liquid chromatography |
| title | Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extract |
| title_full | Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extract |
| title_fullStr | Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extract |
| title_full_unstemmed | Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extract |
| title_short | Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extract |
| title_sort | cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in portulaca oleracea l extract |
| topic | Portulaca oleracea L./hydroxydihydrobovolide Portulaca oleracea L./hydroxydihydrobovolide/cytotoxicity Portulaca oleracea L./hydroxydihydrobovolide/pharmacokinetics Hydroxydihydrobovolide/Ultra-high performance liquid chromatography |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200615&lng=en&tlng=en |
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