Molecular diagnosis and genotyping of Pneumocystis jirovecii in bronchoalveolar lavage samples obtained from patients with pulmonary disorder
Background and Purpose: Pneumocystis pneumonia (PCP) is one of the most common and life-threatening fungal diseases in patients with human immunodeficiency, treated with immunosuppressive medications. Immunocompetent people can also be a spreading agent for PCP. Regarding this, the aim of the presen...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2019-09-01
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| Series: | Current Medical Mycology |
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| Online Access: | http://cmm.mazums.ac.ir/article_96075_a5658adf773b4646ba0df9546a704d54.pdf |
| _version_ | 1818023828094189568 |
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| author | Abdolmajid Fata Bahareh Abdollahi Fariba Rezaeitalab Davood Attaran Mohsen Najjari Mohammad Javad Najafzadeh |
| author_facet | Abdolmajid Fata Bahareh Abdollahi Fariba Rezaeitalab Davood Attaran Mohsen Najjari Mohammad Javad Najafzadeh |
| author_sort | Abdolmajid Fata |
| collection | DOAJ |
| description | Background and Purpose: Pneumocystis pneumonia (PCP) is one of the most common and life-threatening fungal diseases in patients with human immunodeficiency, treated with immunosuppressive medications. Immunocompetent people can also be a spreading agent for PCP. Regarding this, the aim of the present study was to diagnose and identify Pneumocystis jirovecii in bronchoalveolar lavage (BAL) samples obtained from patients with pulmonary disorder using a molecular method.<br /> Materials and Methods: For the purpose of the study, BAL samples (n=138) were collected from patients, undergoing bronchoscopy at the different departments of university hospitals affiliated to Mashhad University of Medical Sciences, Mashhad, Iran, during a period of one year (i.e., April 2014 until May 2015). Giemsa staining and molecular identification were carried out for each sample. The samples were also subjected to nested polymerase chain reaction (PCR), sequencing, and genotyping based on mitochondrial ribosomal large subunit (mtLSU rRNA) of P. jirovecii. The phylogenic tree was constructed by MEGA6 software.<br /> Results: The results of direct microscopic examination revealed the presence of P. jirovecii in 3 (2.2%) out of 138 samples; in addition, nested PCR and sequencing led to the detection of species in 17 (12.3%) samples. Out of patients with positive results, 10 (25%) and 7 (7.1%) cases were immunosuppressed and immunocompetent, respectively. The most common clinical symptoms among patients with pneumocystis were fever, dyspnea, and dry cough. In addition, genotypes III and II were the dominant genotypes in our dataset.<br /> Conclusion: Nested PCR and sequencing methods showed higher sensitivity and specificity as compared with a direct staining technique. Genotype III was identified as the most dominant type in patients with pulmonary disorder in Mashhad. |
| first_indexed | 2024-12-10T03:50:31Z |
| format | Article |
| id | doaj.art-b94e198b89d943cebbffeb6192af6534 |
| institution | Directory Open Access Journal |
| issn | 2423-3439 2423-3420 |
| language | English |
| last_indexed | 2024-12-10T03:50:31Z |
| publishDate | 2019-09-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Current Medical Mycology |
| spelling | doaj.art-b94e198b89d943cebbffeb6192af65342022-12-22T02:03:17ZengMazandaran University of Medical SciencesCurrent Medical Mycology2423-34392423-34202019-09-0153131810.18502/cmm.5.3.174196075Molecular diagnosis and genotyping of Pneumocystis jirovecii in bronchoalveolar lavage samples obtained from patients with pulmonary disorderAbdolmajid Fata0Bahareh Abdollahi1Fariba Rezaeitalab2Davood Attaran3Mohsen Najjari4Mohammad Javad Najafzadeh5Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranBackground and Purpose: Pneumocystis pneumonia (PCP) is one of the most common and life-threatening fungal diseases in patients with human immunodeficiency, treated with immunosuppressive medications. Immunocompetent people can also be a spreading agent for PCP. Regarding this, the aim of the present study was to diagnose and identify Pneumocystis jirovecii in bronchoalveolar lavage (BAL) samples obtained from patients with pulmonary disorder using a molecular method.<br /> Materials and Methods: For the purpose of the study, BAL samples (n=138) were collected from patients, undergoing bronchoscopy at the different departments of university hospitals affiliated to Mashhad University of Medical Sciences, Mashhad, Iran, during a period of one year (i.e., April 2014 until May 2015). Giemsa staining and molecular identification were carried out for each sample. The samples were also subjected to nested polymerase chain reaction (PCR), sequencing, and genotyping based on mitochondrial ribosomal large subunit (mtLSU rRNA) of P. jirovecii. The phylogenic tree was constructed by MEGA6 software.<br /> Results: The results of direct microscopic examination revealed the presence of P. jirovecii in 3 (2.2%) out of 138 samples; in addition, nested PCR and sequencing led to the detection of species in 17 (12.3%) samples. Out of patients with positive results, 10 (25%) and 7 (7.1%) cases were immunosuppressed and immunocompetent, respectively. The most common clinical symptoms among patients with pneumocystis were fever, dyspnea, and dry cough. In addition, genotypes III and II were the dominant genotypes in our dataset.<br /> Conclusion: Nested PCR and sequencing methods showed higher sensitivity and specificity as compared with a direct staining technique. Genotype III was identified as the most dominant type in patients with pulmonary disorder in Mashhad.http://cmm.mazums.ac.ir/article_96075_a5658adf773b4646ba0df9546a704d54.pdfbronchoalveolar lavage (bal)irannested-pcrpneumocystis jiroveciipneumocystis pneumonia (pcp) |
| spellingShingle | Abdolmajid Fata Bahareh Abdollahi Fariba Rezaeitalab Davood Attaran Mohsen Najjari Mohammad Javad Najafzadeh Molecular diagnosis and genotyping of Pneumocystis jirovecii in bronchoalveolar lavage samples obtained from patients with pulmonary disorder Current Medical Mycology bronchoalveolar lavage (bal) iran nested-pcr pneumocystis jirovecii pneumocystis pneumonia (pcp) |
| title | Molecular diagnosis and genotyping of Pneumocystis jirovecii in bronchoalveolar lavage samples obtained from patients with pulmonary disorder |
| title_full | Molecular diagnosis and genotyping of Pneumocystis jirovecii in bronchoalveolar lavage samples obtained from patients with pulmonary disorder |
| title_fullStr | Molecular diagnosis and genotyping of Pneumocystis jirovecii in bronchoalveolar lavage samples obtained from patients with pulmonary disorder |
| title_full_unstemmed | Molecular diagnosis and genotyping of Pneumocystis jirovecii in bronchoalveolar lavage samples obtained from patients with pulmonary disorder |
| title_short | Molecular diagnosis and genotyping of Pneumocystis jirovecii in bronchoalveolar lavage samples obtained from patients with pulmonary disorder |
| title_sort | molecular diagnosis and genotyping of pneumocystis jirovecii in bronchoalveolar lavage samples obtained from patients with pulmonary disorder |
| topic | bronchoalveolar lavage (bal) iran nested-pcr pneumocystis jirovecii pneumocystis pneumonia (pcp) |
| url | http://cmm.mazums.ac.ir/article_96075_a5658adf773b4646ba0df9546a704d54.pdf |
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