Accurate molecular identification of different meat adulterations without carryover contaminations on a microarray chip PCR-directed microfluidic lateral flow strip device
Meat adulteration-based food fraud has recently become one of the global major economical, illegal, religious, and public health concerns. In this work, we developed a microarray chip polymerase chain reaction (PCR)-directed microfluidic lateral flow strip (LFS) device that facilitates the accurate...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Food Chemistry: Molecular Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666566223000205 |
| _version_ | 1797389433423527936 |
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| author | Hanling Wang Xianzhuo Meng Li Yao Qian Wu Bangben Yao Zhaoran Chen Jianguo Xu Wei Chen |
| author_facet | Hanling Wang Xianzhuo Meng Li Yao Qian Wu Bangben Yao Zhaoran Chen Jianguo Xu Wei Chen |
| author_sort | Hanling Wang |
| collection | DOAJ |
| description | Meat adulteration-based food fraud has recently become one of the global major economical, illegal, religious, and public health concerns. In this work, we developed a microarray chip polymerase chain reaction (PCR)-directed microfluidic lateral flow strip (LFS) device that facilitates the accurate and simultaneous identification of beef adulterated with chicken, duck, and pork, especially in processed beef products. To realize this goal, four pairs of amplification primers were designed and applied for specifically amplifying genomic DNA extracted from mixed meat powders in microarray chip. With the prominent advantage of this device lies in the flexible combination and integration of sample loading, detection, and reporting in microstructures, all the DNA amplicons can be individually visualized on the LFS unit, leading to the appearance of test lines (TC line, TD line, TP line, or TB line) as well as the control line (C line) for the species identification and quantification in beef products. Based on this new method, the adulterants were successfully distinguished and identified in mixtures down to 0.01% (wt.%) while the carryover aerogel contamination in routine molecular diagnostic laboratories was effectively avoided. The practicability, accuracy, and reliability of the device were further confirmed by using real-time PCR as a gold standard control on the successful identification of 50 processed ground meat samples sourced from local markets. The method and device proposed herein could be a useful tool for on-site identification of food authentication. |
| first_indexed | 2024-03-08T22:55:54Z |
| format | Article |
| id | doaj.art-b954afd1c93345b2839314fce730e0c3 |
| institution | Directory Open Access Journal |
| issn | 2666-5662 |
| language | English |
| last_indexed | 2024-03-08T22:55:54Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: Molecular Sciences |
| spelling | doaj.art-b954afd1c93345b2839314fce730e0c32023-12-16T06:09:41ZengElsevierFood Chemistry: Molecular Sciences2666-56622023-12-017100180Accurate molecular identification of different meat adulterations without carryover contaminations on a microarray chip PCR-directed microfluidic lateral flow strip deviceHanling Wang0Xianzhuo Meng1Li Yao2Qian Wu3Bangben Yao4Zhaoran Chen5Jianguo Xu6Wei Chen7Engineering Research Center of Bio-process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaEngineering Research Center of Bio-process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food Science and Bioengineering, Changsha University of Science & Technology, Changsha 410114, ChinaEngineering Research Center of Bio-process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaEngineering Research Center of Bio-process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China; Anhui Province Institute of Product Quality Supervision & Inspection, Hefei 230051, ChinaAnhui Province Institute of Product Quality Supervision & Inspection, Hefei 230051, ChinaEngineering Research Center of Bio-process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaEngineering Research Center of Bio-process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China; Corresponding authors.Meat adulteration-based food fraud has recently become one of the global major economical, illegal, religious, and public health concerns. In this work, we developed a microarray chip polymerase chain reaction (PCR)-directed microfluidic lateral flow strip (LFS) device that facilitates the accurate and simultaneous identification of beef adulterated with chicken, duck, and pork, especially in processed beef products. To realize this goal, four pairs of amplification primers were designed and applied for specifically amplifying genomic DNA extracted from mixed meat powders in microarray chip. With the prominent advantage of this device lies in the flexible combination and integration of sample loading, detection, and reporting in microstructures, all the DNA amplicons can be individually visualized on the LFS unit, leading to the appearance of test lines (TC line, TD line, TP line, or TB line) as well as the control line (C line) for the species identification and quantification in beef products. Based on this new method, the adulterants were successfully distinguished and identified in mixtures down to 0.01% (wt.%) while the carryover aerogel contamination in routine molecular diagnostic laboratories was effectively avoided. The practicability, accuracy, and reliability of the device were further confirmed by using real-time PCR as a gold standard control on the successful identification of 50 processed ground meat samples sourced from local markets. The method and device proposed herein could be a useful tool for on-site identification of food authentication.http://www.sciencedirect.com/science/article/pii/S2666566223000205Meat adulterationMicroarray chipMolecular amplificationLateral flow stripSimultaneous identification |
| spellingShingle | Hanling Wang Xianzhuo Meng Li Yao Qian Wu Bangben Yao Zhaoran Chen Jianguo Xu Wei Chen Accurate molecular identification of different meat adulterations without carryover contaminations on a microarray chip PCR-directed microfluidic lateral flow strip device Food Chemistry: Molecular Sciences Meat adulteration Microarray chip Molecular amplification Lateral flow strip Simultaneous identification |
| title | Accurate molecular identification of different meat adulterations without carryover contaminations on a microarray chip PCR-directed microfluidic lateral flow strip device |
| title_full | Accurate molecular identification of different meat adulterations without carryover contaminations on a microarray chip PCR-directed microfluidic lateral flow strip device |
| title_fullStr | Accurate molecular identification of different meat adulterations without carryover contaminations on a microarray chip PCR-directed microfluidic lateral flow strip device |
| title_full_unstemmed | Accurate molecular identification of different meat adulterations without carryover contaminations on a microarray chip PCR-directed microfluidic lateral flow strip device |
| title_short | Accurate molecular identification of different meat adulterations without carryover contaminations on a microarray chip PCR-directed microfluidic lateral flow strip device |
| title_sort | accurate molecular identification of different meat adulterations without carryover contaminations on a microarray chip pcr directed microfluidic lateral flow strip device |
| topic | Meat adulteration Microarray chip Molecular amplification Lateral flow strip Simultaneous identification |
| url | http://www.sciencedirect.com/science/article/pii/S2666566223000205 |
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