Anti-Allergic Effects of Vernonia amygdalina Leaf Extracts in Hapten-Induced Atopic Dermatitis-Like Disease in Mice
Background: Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by pruritic and ec- zematous skin lesions. In this study, AD-like disease was induced in NC/Nga mice so as to evaluate the antiallergic effects of Vernonia amygdalina leaf extracts (VAM). Methods: Forty NC/Nga mi...
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| Format: | Article |
| Language: | English |
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Elsevier
2012-01-01
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| Series: | Allergology International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1323893015302616 |
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| author | Nlandu Roger Ngatu Maiko K. Okajima Maki Yokogawa Ryoji Hirota Mikiro Takaishi Masamitsu Eitoku Basilua Andre Muzembo Asif Bhati Sabah Takao Saruta Mitsuhiko Miyamura Tatsuo Kaneko Shigetoshi Sano Narufumi Suganuma |
| author_facet | Nlandu Roger Ngatu Maiko K. Okajima Maki Yokogawa Ryoji Hirota Mikiro Takaishi Masamitsu Eitoku Basilua Andre Muzembo Asif Bhati Sabah Takao Saruta Mitsuhiko Miyamura Tatsuo Kaneko Shigetoshi Sano Narufumi Suganuma |
| author_sort | Nlandu Roger Ngatu |
| collection | DOAJ |
| description | Background: Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by pruritic and ec- zematous skin lesions. In this study, AD-like disease was induced in NC/Nga mice so as to evaluate the antiallergic effects of Vernonia amygdalina leaf extracts (VAM).
Methods: Forty NC/Nga mice were purchased for each of the two protocols (prophylactic and curative) of the study. Mice were randomly divided in groups of five or six after sensitization with 5% trinitrochlorobenzene (TNCB): aqueous extracts (VAM1), methanolic extracts (VAM2), hydrocortisone (HCT), buffer for the control (TNCB) and the normal mice (NORM) groups.
Results: As for HCT, VAM1 and VAM2-pretreated mice showed significantly lower number of scratching behavior episodes (p<0.01; vs. TNCB) following TNCB challenge. In addition, VAM1, VAM2 exerted a significant inhibitory effect on the development of AD skin symptoms (vs. TNCB group; p<0.001), the production of IgE, TNF-alpha (p<0.05), IL-5 and IFN-gamma (p<0.01) (vs. TNCB group) and on the increase in ear thickness (p<0.05) in prophylactic protocol.
In the AD curative protocol, topical VAM1, VAM2 markedly improved skin lesions such as erythema/hemorrhage (p<0.05), scaling/dryness, erosion/excoriation (p<0.01) (vs. TNCB mice). Furthermore, a significant decrease in ear thickness was noted in VAM1, VAM2, HCT groups (vs. TNCB group; p<0.05) as well as the serum total IgE, MCP-1 (p<0.01) and eotaxin (p<0.05). VAM2 also improved chronic eczema dermatitis skin symptoms in a patient.
Conclusions: Results from this report suggest that VAM extracts, known as ERK pathway inhibitor, prevent and improve atopic/eczema dermatitis syndrome. |
| first_indexed | 2024-12-22T13:52:18Z |
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| institution | Directory Open Access Journal |
| issn | 1323-8930 |
| language | English |
| last_indexed | 2024-12-22T13:52:18Z |
| publishDate | 2012-01-01 |
| publisher | Elsevier |
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| series | Allergology International |
| spelling | doaj.art-b9574e7ef09240bb83135430f841f2f62022-12-21T18:23:38ZengElsevierAllergology International1323-89302012-01-0161459760710.2332/allergolint.11-OA-0393Anti-Allergic Effects of Vernonia amygdalina Leaf Extracts in Hapten-Induced Atopic Dermatitis-Like Disease in MiceNlandu Roger Ngatu0Maiko K. Okajima1Maki Yokogawa2Ryoji Hirota3Mikiro Takaishi4Masamitsu Eitoku5Basilua Andre Muzembo6Asif Bhati Sabah7Takao Saruta8Mitsuhiko Miyamura9Tatsuo Kaneko10Shigetoshi Sano11Narufumi Suganuma12Department of Environmental MedicineDepartment of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa, Japan.Department of DermatologyDepartment of Environmental MedicineDepartment of DermatologyDepartment of Environmental MedicineDepartment of Environmental MedicineDepartment of Environmental MedicineSaruta Clinic of Dermatology, KochiDepartment of Pharmacy, Kochi University Medical SchoolDepartment of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa, Japan.Department of DermatologyDepartment of Environmental MedicineBackground: Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by pruritic and ec- zematous skin lesions. In this study, AD-like disease was induced in NC/Nga mice so as to evaluate the antiallergic effects of Vernonia amygdalina leaf extracts (VAM). Methods: Forty NC/Nga mice were purchased for each of the two protocols (prophylactic and curative) of the study. Mice were randomly divided in groups of five or six after sensitization with 5% trinitrochlorobenzene (TNCB): aqueous extracts (VAM1), methanolic extracts (VAM2), hydrocortisone (HCT), buffer for the control (TNCB) and the normal mice (NORM) groups. Results: As for HCT, VAM1 and VAM2-pretreated mice showed significantly lower number of scratching behavior episodes (p<0.01; vs. TNCB) following TNCB challenge. In addition, VAM1, VAM2 exerted a significant inhibitory effect on the development of AD skin symptoms (vs. TNCB group; p<0.001), the production of IgE, TNF-alpha (p<0.05), IL-5 and IFN-gamma (p<0.01) (vs. TNCB group) and on the increase in ear thickness (p<0.05) in prophylactic protocol. In the AD curative protocol, topical VAM1, VAM2 markedly improved skin lesions such as erythema/hemorrhage (p<0.05), scaling/dryness, erosion/excoriation (p<0.01) (vs. TNCB mice). Furthermore, a significant decrease in ear thickness was noted in VAM1, VAM2, HCT groups (vs. TNCB group; p<0.05) as well as the serum total IgE, MCP-1 (p<0.01) and eotaxin (p<0.05). VAM2 also improved chronic eczema dermatitis skin symptoms in a patient. Conclusions: Results from this report suggest that VAM extracts, known as ERK pathway inhibitor, prevent and improve atopic/eczema dermatitis syndrome.http://www.sciencedirect.com/science/article/pii/S1323893015302616atopic dermatitiseosinophiliascratchVernonia amygdalina |
| spellingShingle | Nlandu Roger Ngatu Maiko K. Okajima Maki Yokogawa Ryoji Hirota Mikiro Takaishi Masamitsu Eitoku Basilua Andre Muzembo Asif Bhati Sabah Takao Saruta Mitsuhiko Miyamura Tatsuo Kaneko Shigetoshi Sano Narufumi Suganuma Anti-Allergic Effects of Vernonia amygdalina Leaf Extracts in Hapten-Induced Atopic Dermatitis-Like Disease in Mice Allergology International atopic dermatitis eosinophilia scratch Vernonia amygdalina |
| title | Anti-Allergic Effects of Vernonia amygdalina Leaf Extracts in Hapten-Induced Atopic Dermatitis-Like Disease in Mice |
| title_full | Anti-Allergic Effects of Vernonia amygdalina Leaf Extracts in Hapten-Induced Atopic Dermatitis-Like Disease in Mice |
| title_fullStr | Anti-Allergic Effects of Vernonia amygdalina Leaf Extracts in Hapten-Induced Atopic Dermatitis-Like Disease in Mice |
| title_full_unstemmed | Anti-Allergic Effects of Vernonia amygdalina Leaf Extracts in Hapten-Induced Atopic Dermatitis-Like Disease in Mice |
| title_short | Anti-Allergic Effects of Vernonia amygdalina Leaf Extracts in Hapten-Induced Atopic Dermatitis-Like Disease in Mice |
| title_sort | anti allergic effects of vernonia amygdalina leaf extracts in hapten induced atopic dermatitis like disease in mice |
| topic | atopic dermatitis eosinophilia scratch Vernonia amygdalina |
| url | http://www.sciencedirect.com/science/article/pii/S1323893015302616 |
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