Effects of Diabetes on Microcirculation and Leukostasis in Retinal and Non-Ocular Tissues: Implications for Diabetic Retinopathy
Changes in retinal microcirculation are associated with the development of diabetic retinopathy (DR). However, it is unclear whether such changes also develop in capillary beds of other non-retinal tissues. Here, we investigated microcirculatory changes involving velocity of rolling neutrophils, adh...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/10/11/1583 |
| _version_ | 1827701779686490112 |
|---|---|
| author | Ana Silva Herdade Iara Mota Silva Ângelo Calado Carlota Saldanha Ngan-Ha Nguyen Isabella Hou Miguel Castanho Sayon Roy |
| author_facet | Ana Silva Herdade Iara Mota Silva Ângelo Calado Carlota Saldanha Ngan-Ha Nguyen Isabella Hou Miguel Castanho Sayon Roy |
| author_sort | Ana Silva Herdade |
| collection | DOAJ |
| description | Changes in retinal microcirculation are associated with the development of diabetic retinopathy (DR). However, it is unclear whether such changes also develop in capillary beds of other non-retinal tissues. Here, we investigated microcirculatory changes involving velocity of rolling neutrophils, adherence of neutrophils, and leukostasis during development of retinal vascular lesions in diabetes in other non-retinal tissues. Intravital microscopy was performed on post-capillary venules of cremaster muscle and ear lobe of mice with severe or moderate diabetes and compared to those of non-diabetic mice. Additionally, number and velocity of rolling leukocytes, number of adherent leukocytes, and areas of leukostasis were quantified, and retinal capillary networks were examined for acellular capillaries (AC) and pericyte loss (PL), two prominent vascular lesions characteristic of DR. The number of adherent neutrophils and areas of leukostasis in the cremaster and ear lobe post-capillary venules of diabetic mice was increased compared to those of non-diabetic mice. Similarly, a significant increase in the number of rolling neutrophils and decrease in their rolling velocities compared to those of non-diabetic control mice were observed and severity of diabetes exacerbated these changes. Understanding diabetes-induced microcirculatory changes in cremaster and ear lobe may provide insight into retinal vascular lesion development in DR. |
| first_indexed | 2024-03-10T14:41:15Z |
| format | Article |
| id | doaj.art-b95787445d634ee1b635e6b8b3c8c4ab |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T14:41:15Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-b95787445d634ee1b635e6b8b3c8c4ab2023-11-20T21:50:04ZengMDPI AGBiomolecules2218-273X2020-11-011011158310.3390/biom10111583Effects of Diabetes on Microcirculation and Leukostasis in Retinal and Non-Ocular Tissues: Implications for Diabetic RetinopathyAna Silva Herdade0Iara Mota Silva1Ângelo Calado2Carlota Saldanha3Ngan-Ha Nguyen4Isabella Hou5Miguel Castanho6Sayon Roy7Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa 1649-004, PortugalInstituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa 1649-004, PortugalInstituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa 1649-004, PortugalInstituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa 1649-004, PortugalDepartment of Medicine and Ophthalmology, Boston University School of Medicine, Boston, MA 02118, USADepartment of Medicine and Ophthalmology, Boston University School of Medicine, Boston, MA 02118, USAInstituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa 1649-004, PortugalDepartment of Medicine and Ophthalmology, Boston University School of Medicine, Boston, MA 02118, USAChanges in retinal microcirculation are associated with the development of diabetic retinopathy (DR). However, it is unclear whether such changes also develop in capillary beds of other non-retinal tissues. Here, we investigated microcirculatory changes involving velocity of rolling neutrophils, adherence of neutrophils, and leukostasis during development of retinal vascular lesions in diabetes in other non-retinal tissues. Intravital microscopy was performed on post-capillary venules of cremaster muscle and ear lobe of mice with severe or moderate diabetes and compared to those of non-diabetic mice. Additionally, number and velocity of rolling leukocytes, number of adherent leukocytes, and areas of leukostasis were quantified, and retinal capillary networks were examined for acellular capillaries (AC) and pericyte loss (PL), two prominent vascular lesions characteristic of DR. The number of adherent neutrophils and areas of leukostasis in the cremaster and ear lobe post-capillary venules of diabetic mice was increased compared to those of non-diabetic mice. Similarly, a significant increase in the number of rolling neutrophils and decrease in their rolling velocities compared to those of non-diabetic control mice were observed and severity of diabetes exacerbated these changes. Understanding diabetes-induced microcirculatory changes in cremaster and ear lobe may provide insight into retinal vascular lesion development in DR.https://www.mdpi.com/2218-273X/10/11/1583microcirculationleukostasisdiabetic retinopathy |
| spellingShingle | Ana Silva Herdade Iara Mota Silva Ângelo Calado Carlota Saldanha Ngan-Ha Nguyen Isabella Hou Miguel Castanho Sayon Roy Effects of Diabetes on Microcirculation and Leukostasis in Retinal and Non-Ocular Tissues: Implications for Diabetic Retinopathy Biomolecules microcirculation leukostasis diabetic retinopathy |
| title | Effects of Diabetes on Microcirculation and Leukostasis in Retinal and Non-Ocular Tissues: Implications for Diabetic Retinopathy |
| title_full | Effects of Diabetes on Microcirculation and Leukostasis in Retinal and Non-Ocular Tissues: Implications for Diabetic Retinopathy |
| title_fullStr | Effects of Diabetes on Microcirculation and Leukostasis in Retinal and Non-Ocular Tissues: Implications for Diabetic Retinopathy |
| title_full_unstemmed | Effects of Diabetes on Microcirculation and Leukostasis in Retinal and Non-Ocular Tissues: Implications for Diabetic Retinopathy |
| title_short | Effects of Diabetes on Microcirculation and Leukostasis in Retinal and Non-Ocular Tissues: Implications for Diabetic Retinopathy |
| title_sort | effects of diabetes on microcirculation and leukostasis in retinal and non ocular tissues implications for diabetic retinopathy |
| topic | microcirculation leukostasis diabetic retinopathy |
| url | https://www.mdpi.com/2218-273X/10/11/1583 |
| work_keys_str_mv | AT anasilvaherdade effectsofdiabetesonmicrocirculationandleukostasisinretinalandnonoculartissuesimplicationsfordiabeticretinopathy AT iaramotasilva effectsofdiabetesonmicrocirculationandleukostasisinretinalandnonoculartissuesimplicationsfordiabeticretinopathy AT angelocalado effectsofdiabetesonmicrocirculationandleukostasisinretinalandnonoculartissuesimplicationsfordiabeticretinopathy AT carlotasaldanha effectsofdiabetesonmicrocirculationandleukostasisinretinalandnonoculartissuesimplicationsfordiabeticretinopathy AT nganhanguyen effectsofdiabetesonmicrocirculationandleukostasisinretinalandnonoculartissuesimplicationsfordiabeticretinopathy AT isabellahou effectsofdiabetesonmicrocirculationandleukostasisinretinalandnonoculartissuesimplicationsfordiabeticretinopathy AT miguelcastanho effectsofdiabetesonmicrocirculationandleukostasisinretinalandnonoculartissuesimplicationsfordiabeticretinopathy AT sayonroy effectsofdiabetesonmicrocirculationandleukostasisinretinalandnonoculartissuesimplicationsfordiabeticretinopathy |