The Avian Germline and Strategies for the Production of Transgenic Chickens
Considerable interest exists in the development of transgenic poultry as a means of improving commercial poultry stocks and for applications in the pharmaceutical industry. The development of transgenic poultry requires stable insertion of genes into the germline. Because of the unique aspects of re...
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2002-10-01
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| Series: | The Journal of Poultry Science |
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| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/39/4/39_4_205/_pdf/-char/en |
| _version_ | 1797816963329687552 |
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| author | James N. Petitte |
| author_facet | James N. Petitte |
| author_sort | James N. Petitte |
| collection | DOAJ |
| description | Considerable interest exists in the development of transgenic poultry as a means of improving commercial poultry stocks and for applications in the pharmaceutical industry. The development of transgenic poultry requires stable insertion of genes into the germline. Because of the unique aspects of reproduction in birds, new methods of embryo manipulation had to be developed that include the ex ovo culture of embryos and the formation of germline chimeras using blastodermal cells and primordial germ cells. Currently, transgenic poultry can be produced using retroviral vectors and through the microinjection of DNA into the germinal disk of the newly fertilized egg. However, other approaches such as the development of embryonic stem cells, the culture of primordial germ cells, and sperm-mediated transfection are being explored for their potential to produce transgenic poultry. |
| first_indexed | 2024-03-13T08:45:25Z |
| format | Article |
| id | doaj.art-b95d150048d14046943bd899bab5d697 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:45:25Z |
| publishDate | 2002-10-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-b95d150048d14046943bd899bab5d6972023-05-30T05:41:29ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862002-10-0139420522810.2141/jpsa.39.205jpsaThe Avian Germline and Strategies for the Production of Transgenic ChickensJames N. Petitte0Department of Poultry Science College of Agriculture and Life Sciences, NC State University, RaleighConsiderable interest exists in the development of transgenic poultry as a means of improving commercial poultry stocks and for applications in the pharmaceutical industry. The development of transgenic poultry requires stable insertion of genes into the germline. Because of the unique aspects of reproduction in birds, new methods of embryo manipulation had to be developed that include the ex ovo culture of embryos and the formation of germline chimeras using blastodermal cells and primordial germ cells. Currently, transgenic poultry can be produced using retroviral vectors and through the microinjection of DNA into the germinal disk of the newly fertilized egg. However, other approaches such as the development of embryonic stem cells, the culture of primordial germ cells, and sperm-mediated transfection are being explored for their potential to produce transgenic poultry.https://www.jstage.jst.go.jp/article/jpsa/39/4/39_4_205/_pdf/-char/enprimordial germ cellstransgenicchickenschimerasblastoderm |
| spellingShingle | James N. Petitte The Avian Germline and Strategies for the Production of Transgenic Chickens The Journal of Poultry Science primordial germ cells transgenic chickens chimeras blastoderm |
| title | The Avian Germline and Strategies for the Production of Transgenic Chickens |
| title_full | The Avian Germline and Strategies for the Production of Transgenic Chickens |
| title_fullStr | The Avian Germline and Strategies for the Production of Transgenic Chickens |
| title_full_unstemmed | The Avian Germline and Strategies for the Production of Transgenic Chickens |
| title_short | The Avian Germline and Strategies for the Production of Transgenic Chickens |
| title_sort | avian germline and strategies for the production of transgenic chickens |
| topic | primordial germ cells transgenic chickens chimeras blastoderm |
| url | https://www.jstage.jst.go.jp/article/jpsa/39/4/39_4_205/_pdf/-char/en |
| work_keys_str_mv | AT jamesnpetitte theaviangermlineandstrategiesfortheproductionoftransgenicchickens AT jamesnpetitte aviangermlineandstrategiesfortheproductionoftransgenicchickens |