A MYB-related transcription factor from peanut, AhMYB30, improves freezing and salt stress tolerance in transgenic Arabidopsis through both DREB/CBF and ABA-signaling pathways
Abiotic stresses such as salinity and low temperature have serious impact on peanut growth and yield. The present work investigated the function of a MYB-related transcription factor gene AhMYB30 obtained from peanut under salt and low temperature stresses by transgenic methods. The results indicate...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1136626/full |
| _version_ | 1811163388328280064 |
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| author | Na Chen Lijuan Pan Zhen Yang Maowen Su Jing Xu Xiao Jiang Xiangzhen Yin Tong Wang Feifei Wan Xiaoyuan Chi |
| author_facet | Na Chen Lijuan Pan Zhen Yang Maowen Su Jing Xu Xiao Jiang Xiangzhen Yin Tong Wang Feifei Wan Xiaoyuan Chi |
| author_sort | Na Chen |
| collection | DOAJ |
| description | Abiotic stresses such as salinity and low temperature have serious impact on peanut growth and yield. The present work investigated the function of a MYB-related transcription factor gene AhMYB30 obtained from peanut under salt and low temperature stresses by transgenic methods. The results indicated that the overexpression of AhMYB30 in Arabidopsis could enhance the resistance of transgenic plants to freezing and salt stresses. The expression of stress-response genes RD29A (Response-to-Dehydration 29A), COR15A (Cold-Regulated 15A), KIN1 (Kinesin 1) and ABI2 (Abscisic acid Insensitive 2) increased in transgenic plants compared with in wild-type. Subcellular localization and transcriptional autoactivation validation demonstrated that AhMYB30 has essential features of transcription factors. Therefore, AhMYB30 may increase salt and freezing stress tolerance as the transcription factor (TF) in Arabidopsis through both DREB/CBF and ABA-signaling pathways. Our results lay the theoretical foundation for exploring stress resistance mechanisms of peanut and offering novel genetic resources for molecular breeding. |
| first_indexed | 2024-04-10T06:44:21Z |
| format | Article |
| id | doaj.art-b95d8305dbc84fd1ac4afd724f9c6f81 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T06:44:21Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-b95d8305dbc84fd1ac4afd724f9c6f812023-02-28T15:00:43ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-02-011410.3389/fpls.2023.11366261136626A MYB-related transcription factor from peanut, AhMYB30, improves freezing and salt stress tolerance in transgenic Arabidopsis through both DREB/CBF and ABA-signaling pathwaysNa Chen0Lijuan Pan1Zhen Yang2Maowen Su3Jing Xu4Xiao Jiang5Xiangzhen Yin6Tong Wang7Feifei Wan8Xiaoyuan Chi9Key Laboratory of Peanut Biology, Genetic & Breeding, Ministry of Agriculture and Rural Affairs, Shandong Peanut Research Institute, Qingdao, ChinaKey Laboratory of Peanut Biology, Genetic & Breeding, Ministry of Agriculture and Rural Affairs, Shandong Peanut Research Institute, Qingdao, ChinaKey Laboratory of Peanut Biology, Genetic & Breeding, Ministry of Agriculture and Rural Affairs, Shandong Peanut Research Institute, Qingdao, ChinaDepartment of Animal and Plant Quarantine, Qingdao Customs, Qingdao, ChinaKey Laboratory of Peanut Biology, Genetic & Breeding, Ministry of Agriculture and Rural Affairs, Shandong Peanut Research Institute, Qingdao, ChinaKey Laboratory of Peanut Biology, Genetic & Breeding, Ministry of Agriculture and Rural Affairs, Shandong Peanut Research Institute, Qingdao, ChinaKey Laboratory of Peanut Biology, Genetic & Breeding, Ministry of Agriculture and Rural Affairs, Shandong Peanut Research Institute, Qingdao, ChinaKey Laboratory of Peanut Biology, Genetic & Breeding, Ministry of Agriculture and Rural Affairs, Shandong Peanut Research Institute, Qingdao, ChinaDivision for Guidance of Cooperative Economy, Binzhou Agricultural Technology Extension Center, Binzhou, ChinaKey Laboratory of Peanut Biology, Genetic & Breeding, Ministry of Agriculture and Rural Affairs, Shandong Peanut Research Institute, Qingdao, ChinaAbiotic stresses such as salinity and low temperature have serious impact on peanut growth and yield. The present work investigated the function of a MYB-related transcription factor gene AhMYB30 obtained from peanut under salt and low temperature stresses by transgenic methods. The results indicated that the overexpression of AhMYB30 in Arabidopsis could enhance the resistance of transgenic plants to freezing and salt stresses. The expression of stress-response genes RD29A (Response-to-Dehydration 29A), COR15A (Cold-Regulated 15A), KIN1 (Kinesin 1) and ABI2 (Abscisic acid Insensitive 2) increased in transgenic plants compared with in wild-type. Subcellular localization and transcriptional autoactivation validation demonstrated that AhMYB30 has essential features of transcription factors. Therefore, AhMYB30 may increase salt and freezing stress tolerance as the transcription factor (TF) in Arabidopsis through both DREB/CBF and ABA-signaling pathways. Our results lay the theoretical foundation for exploring stress resistance mechanisms of peanut and offering novel genetic resources for molecular breeding.https://www.frontiersin.org/articles/10.3389/fpls.2023.1136626/fullpeanut (Arachis hypogaea L.)MYB-related transcription factorcold and salt stressDREB/CBF signaling pathwayABA-signaling pathway |
| spellingShingle | Na Chen Lijuan Pan Zhen Yang Maowen Su Jing Xu Xiao Jiang Xiangzhen Yin Tong Wang Feifei Wan Xiaoyuan Chi A MYB-related transcription factor from peanut, AhMYB30, improves freezing and salt stress tolerance in transgenic Arabidopsis through both DREB/CBF and ABA-signaling pathways Frontiers in Plant Science peanut (Arachis hypogaea L.) MYB-related transcription factor cold and salt stress DREB/CBF signaling pathway ABA-signaling pathway |
| title | A MYB-related transcription factor from peanut, AhMYB30, improves freezing and salt stress tolerance in transgenic Arabidopsis through both DREB/CBF and ABA-signaling pathways |
| title_full | A MYB-related transcription factor from peanut, AhMYB30, improves freezing and salt stress tolerance in transgenic Arabidopsis through both DREB/CBF and ABA-signaling pathways |
| title_fullStr | A MYB-related transcription factor from peanut, AhMYB30, improves freezing and salt stress tolerance in transgenic Arabidopsis through both DREB/CBF and ABA-signaling pathways |
| title_full_unstemmed | A MYB-related transcription factor from peanut, AhMYB30, improves freezing and salt stress tolerance in transgenic Arabidopsis through both DREB/CBF and ABA-signaling pathways |
| title_short | A MYB-related transcription factor from peanut, AhMYB30, improves freezing and salt stress tolerance in transgenic Arabidopsis through both DREB/CBF and ABA-signaling pathways |
| title_sort | myb related transcription factor from peanut ahmyb30 improves freezing and salt stress tolerance in transgenic arabidopsis through both dreb cbf and aba signaling pathways |
| topic | peanut (Arachis hypogaea L.) MYB-related transcription factor cold and salt stress DREB/CBF signaling pathway ABA-signaling pathway |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1136626/full |
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